Download Fig. 1a standard 0 , 0.1 , 0.2 , 0.5 , 1.0 , 2.0 , 3.0 , 4.0 , 5.0 µmole/L

ordetaund
dies
perafter
ns).
Fast Mimicking of Phase 1 and 2 Metabolism of
Acetaminophen using the ROXY™ EC System
Determination of Iodide in Urine
HPLC and ECD conditions
Mobile phase
Dissolve 10 mM di-Natrium-hydrogenphosphat-Dodecahydrat (1.79 g) in 300 mL
Current
11.09 Iodide 1
water, set pH on 6.80 with H3PO4.
References
Add 1W.,
mM
Hexadecyltrimethylammonium
hydroxide (0.15 g).
1. Lohmann
Karst
U., “Simulation of the detoxification
Add 160 mL
Acetonitril.
of paracetamol
using
on-line electrochemistry/liquid
chromatography/mass
spectrometry”,
Dissolve everything
and fill with Anal.
waterBioanal.
up to 500 mL
Chem.,
386 (2006)
1701-1708
Column
ALF-315,
150x3.0
mm, 3.0 µm C18; 10 uL injection
2. Lohmann W., Hayen H., Karst U., “Covalent Protein
I Cell
around -20 to 50 nA (adjust the potential when current is too high or low)
Modification by Reactive Drug Metabolites Using
DC mode, Ecell: -0.07V, Range: 10 nA; Offsett: 0%; Filter: 0.5 Hz
Figure 10: ROXY™ EC System consisting of ROXY Potentiostat, dual s
Online Electrochemistry/Liquid Chromatography/Mass
and the
ReactorCell.
Sample PrepSpectrometry”,
Dilute the Anal.
urineChem.,
sample8010
or 5 9714-9719
times in water and thenpump
filtered
solution.
(2008)
3. Permentier H. P., Bruins A. P., Bischoff R., ElectrochemistryOrdering number
Mass Spectrometry in Drug Metabolism and Protein
210.0070A
ROXY™ EC system, incl. dual syringe pump, Reacto
Research, Mini-Rev. Med. Chem., 8 (2008) 46-56
[nA]
electrodes and LC connection kit for phase I and II
[nA]
4. Jurva U., Wikstrom H. V., Weidolf L., Bruins A.P., Comparison
tions. All parts included for described Electroche
(EC) application.
between electrochemistry/mass spectrometry and
0
cytochrome P450 catalyzed oxidation reactions,5 Rapid
Commun. Mass Spectrom., 17 (2003) 800-810
-10
5. W. Lohmann, B. Meermann, I. Moller, A. Scheffer,0 U. Karst,
Quantification of Electrochemically Generated Iodine-20
Containing Metabolites Using Inductively Coupled
Plasma
-5
Mass Spectrometry, Anal. Chem., 80 (2008) 9769-9775
6. Huber Ch., Bartha B., Harpaintner R., Schröder P.,
-30
-10
Metabolism of acetaminophen (paracetamol) in plants–
0
2
4
6
8
10
2
4
6
8
10
[min.]
two independent
pathways result in[min.]
the formation of a
Time
Time
glutathione
glucose
Pollut. Iodide. Fig. 1b. On the right side a triplo
Fig. 1a standard
0 , 0.1 ,and
0.2 a, 0.5
, 1.0conjugate,
, 2.0 , 3.0 ,Environ.
4.0 , 5.0Sci.
µmole/L
Res.,16
(2009)
206-213
injection of 10x
diluted
Urine
sample. The peak corresponds to about 2 umole/L iodide end concentration.
Current
(500 mL)
60.00
Height (nA)
XY™
prondly
rget
eutiative
ons),
bolic
ACHTERZIJDE
L
N VOUWEN
40.00
20.00
Distributor:
0.00
0
5
10
nr.
15
20
25
ERC GmbH
☎ +49 89 66055696
Fax +49 89 60824826
[email protected]
www.erc-hplc.de
Figure 2 reproducibility of a 5 µmole/L Iodide injection.
Ihr Spezialist in der LC
www.myAntec.com
®