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Cell Respiration The cell needs something in order to eat, breathe, reproduce, move, and much more…….. How do the cells get the energy they need? • Chemical compounds we call FOOD! Convert chemical energy stored in food into compounds that are more convenient for the cell to use Has 2 membranes - Inner membrane - Lots of FOLDS (cristae)= INCREASE surface area= more ATP being produced - Outer membrane In Animal AND Plant cells Nearly all come from the ovum You get your mitochondria from your mom! Adenosine Triphosphate: nucleotide with two extra groups • Nitrogenous base- adenine • Sugar- ribose • 3 Phosphate groups- bound to ribosome, phosphate tail holds usable energy • Body’s currency for energy Partially charged that can be fully charged by the addition of a phosphate group. > Even if the equation looks simple, Cellular respiration is done in a series of steps. 1. When energy is needed somewhere in the cell 2. Chemical energy stored in glucose is released and used to produce adenosine triphosphate, ATP 3. ATP, is used for short term energy storage and transport in the cell 4. Energy is transferred from Glucose to ATP 5. Then ATP delivers the energy to the places in the cell that need it. 6. This is all done by a long and complicated process called Cell Respiration. • High energy electrons produced in these processes are highly reactive and require a special “carrier.” • Think of it like a hot potato straight from the oven. • If you had to take it out of the oven, you would get a mitt—a carrier—to transport it. NADP+: accepts and holds 2 high-energy electrons, a long with a hydrogen ion. This converts NADP+ to NADPH This is one way in which energy from the sun can be trapped in chemical form and transferred to other parts of the cell. FAD and FADH2 are also electron carriers process by which cells release the energy stored in the bonds of food molecules. From sugars, fats, amino acids, and nucleotides. However, GLUCOSE is the most important source of energy in cells. Aerobic process- requiring oxygen Glycolysis Glucose Electron Transport Chain Krebs Cycle 2 Pyruvic Acid CO2 +NADH + FADH2 H2O + 36 ATP • Occurs in CYTOPLASM • NO OXYGEN After Glycolysis occurs: • 2 Pyruvic acids enter the Krebs Cycle • 2 NADH goes to Electron transport chain • 4 ATP produced • (Net gain of 2 per glucose, since it took 2 ATP to start glycolysis) Glycolysis • Occurs in the MATRIX • Also known as citric acid cycle because Citric acid is the first compound formed As Krebs Cycle Occurs: • 3 CO2 is formed and released into the air • 2 ATP molecules formed • Electron carriers: NADH and FADH2 continue to Electron Transport Chain • • Reminder: Two pyruvic acids enter the Krebs Cycle, so all of the above are doubled Hint: The 4 carbon compound that is initially in the process comes from the citric acid being broken down from a 6 carbon to a 5 then 4 carbon compound. Krebs Cycle • Occurs in Cristae (inner membrane) • Uses the high-energy electrons from glycolysis and the Krebs Cycle to convert ADP into ATP. • For every 2 Electrons that travel down the chain, their energy helps transport hydrogen ions across into the intermembrane space • Charge difference forces Hydrogen ions through ATP synthase channels which makes the enzyme spin. • With each rotation, the enzyme grabs an ADP and attaches a phosphate group, making ATP. • For every 2 Electrons= 3 ATP Electron Transport Chain Site of Cell Respiration: requires oxygen (AEROBIC) “Cell Respiration Song” Three stages: 1. Glycolysis 1. Makes some ATP (4 to be exact) 2. Oxygen NOT needed 3. Location: CYTOPLASM 2. Kreb’s Cycle 1. Makes some ATP 2. Oxygen needed 3. MATRIX OF MITOCHONDRIA 3. Electron Transport Chain 1. Makes ALOT of ATP 2. Oxygen needed 3. Location: Inner membrane of Mitochondria(CRISTAE) Aerobic Anaerobic “in air” “without air” REQUIRES OXYGEN DOES NOT REQUIRE Krebs cycle and Electron Transport Chain OXYGEN Glycolysis Fermentation NO OXYGEN= FERMENTATION -If there is no oxygen available, cell switches to fermentation -Fermentation is Glycolysis over and over again - Happens in cytoplasm - Makes some ATP, but can do so quickly -Alcohol Fermentation -Bacteria and Fungi -Such as yeast -Produce ethyl alcohol and CO2 - Lactic Acid Fermentation -In humans and mammals -After exercising, you feel really sore -It’s the lactic acid build up in your muscles Yeast is a one-celled fungus that converts sugar and starch into carbon dioxide bubbles and alcohol. Cells get energy by: • • • • • • Herbivores Omnivores Carnivores Decomposers detritivores Scavengers Autotroph Heterotroph • Obtain food by consuming other living things • Consumers: • Make their own food • Producers •Plant and bacteria cells only (NEVER in animal cells) •Captures energy from the sunlight and convert it into chemical energy PHOTOSYNTHESIS • Two membranes: > inside has large stacks of other membranes that contain chlorophyll Chloroplast Cellular organelle where photosynthesis occurs Double membrane Outer membrane Inner membrane Stroma (fluid filled space) Granum (stack of thylakoids) Innermembrane space Thylakoids Contains CHLOROPHYLL Contain chemical compound called chlorophyll-gives plants their green color 1. Stomata (PLANT) 1. Small pores in the underside of leaves that release water and oxygen and take in carbon dioxide 2. Guard cells (PLANT) 1. Control the opening and closing of stomata depending on environment 3. Stroma (CHLOROPLAST) 1. Thick fluid enclosed by the inner membrane 4. Thylakoids (CHLOROPLAST) 1. Disc-like sacs suspended in the stroma 2. Has membrane that surrounds inner thylakoid space 5. Grana (sing. Granum) (CHLOROPLAST) 1. Stacks of thylakoids Does this equation look familiar? VS. EVERYTHING IS MADE POSSIBLE BY THE SUN AND THE PROCESS OF PHOTOSYNTHESIS! • Energy from the sun travels to the earth in the form of light. • Sunlight, “white light” is actually a mixture of different wavelengths. • Our eyes see the different wavelengths of the visible spectrum as different colors: red, orange, yellow, green, blue, indigo, and violet. • Plants gather the sun’s energy with light-absorbing molecules called PIGMENTS. • Plant’s principle pigment is CHLOROPHYLL • Chlorophyll a & Chlorophyll b absorb light in the blueviolet and red regions. • DOES NOT absorb light well in the green region, it reflects that color which is why we see plants as green. -Carotene -Secondary plant pigment - Red and orange pigments -Absorb light in other regions of the spectrum -So it reflects orange and red back to us Light-Dependent Reaction Light-Independent Reaction 1. use energy absorbed from chlorophyll and to produce ATP and NADPH 1. CALVIN CYCLE 2. Series of reactions that form glucose from CO2 and Water. Lyn Margulis, American Biologist said that chloroplasts descended from prokaryotes Organelle DNA • Mitochondria and Chloroplasts contain their own genetic information • In a form of small, circular DNA molecules Ancient Prokaryotes from way back in the day had a symbiotic relationship with the ancient eukaryotes What is symbiosis? • A relationship of mutual benefit or dependence. •The prokaryotes lived inside the eukaryotes •There were prokaryotes that used oxygen to make energy (ATP) •Mitochondria • There were prokaryotes that used photosynthesis to get energy •Chloroplasts AGAIN, DO YOU SEE THE SIMILARITIES?