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Bedingungen:
2% TBE
90 V 1h 50min
9 µl PCR-Probe
2 µl Ladepuffer
RT-PCR: LMO7, T58 T59 (397 bp)
M 1 2 3 4 5
6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 M
M25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 M
031007_Gewebe_1_B6_T58T59_calb
M = Marker
1 = Oesophagus
2 = Stomach
3 = Stomach Tumor
4 = Corpus
5 = Antrum
6 = Small Intestine
7 = Duodenum
8 = Large Intestine
9 = Large Intestine
10 = Coecum
11 = Coecum
12 = Colon
13 = Colon Tumor
14 = Rectum
15 = Rectum
16 = Cervix
17 = Endometrium
18 = Uterus
19 = Uterus Tumor
20 = Ovary
21 = Ovary Tumor
22 = Ovary Tumor
23 = Placenta
24 = Testis
25 = Testis Tumor
26 = Prostate gland
27 = Prostate gland
28 = Adrenal gland
29 = Adrenal gland
30 = Kidney
31 = Kidney Fetal
32 = Kidney Tumor
33 = Kidney Tumor
34 = Bladder
35 = Spleen
36 = Spleen
37 = Spleen Fetal
38 = Liver
39 = Liver
40 = Liver Fetal
41 = Pancreas
42 = Pancreas
43 = Salivary gland
RT-PCR: LMO7, T58 T59 (397 bp)
M 1
2
3 4
5
6 7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 M
031007_Gewebe_2_B6_T58T59_calb
Bedingungen:
2% TBE
90 V 1h 45 min
9 µl PCR-Probe
2 µl Ladepuffer
M = Marker
1 = Thyroid gland
2 = Thymus
3 = Thymus Fetal
4 = Tonsil
5 = Tonsil
6 = Lymph nodes Tumor
7 = Marrow
8 = Spinal Cord
9 = Brain
10 = Brain Fetal
11 = H2O
12 = Heart
13 = Heart Fetal
14 = Lung
15 = Lung
16 = Lung Tumor
17 = Breast
18 = Breast
19 = Breast Tumor
20 = Muscle
21 = Muscle
22 = Retina
Bedingungen:
2% TBE
90 V 1h 50min
9 µl PCR-Probe
2 µl Ladepuffer
RT-PCR: LMO7, T58 T60 (507 bp)
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 M
M 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 M
031007_Gewebe_1_B7_T58T60_calb
M = Marker
1 = Oesophagus
2 = Stomach
3 = Stomach Tumor
4 = Corpus
5 = Antrum
6 = Small Intestine
7 = Duodenum
8 = Large Intestine
9 = Large Intestine
10 = Coecum
11 = Coecum
12 = Colon
13 = Colon Tumor
14 = Rectum
15 = Rectum
16 = Cervix
17 = Endometrium
18 = Uterus
19 = Uterus Tumor
20 = Ovary
21 = Ovary Tumor
22 = Ovary Tumor
23 = Placenta
24 = Testis
25 = Testis Tumor
26 = Prostate gland
27 = Prostate gland
28 = Adrenal gland
29 = Adrenal gland
30 = Kidney
31 = Kidney Fetal
32 = Kidney Tumor
33 = Kidney Tumor
34 = Bladder
35 = Spleen
36 = Spleen
37 = Spleen Fetal
38 = Liver
39 = Liver
40 = Liver Fetal
41 = Pancreas
42 = Pancreas
43 = Salivary gland
Bedingungen:
2% TBE
90 V 1h 45 min
9 µl PCR-Probe
2 µl Ladepuffer
RT-PCR: LMO7, T58 T60 (507 bp)
M 1
2
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 M
031007_Gewebe_2_B7_T58T60_calb
M = Marker
1 = Thyroid gland
2 = Thymus
3 = Thymus Fetal
4 = Tonsil
5 = Tonsil
6 = Lymph nodes Tumor
7 = Marrow
8 = Spinal Cord
9 = Brain
10 = Brain Fetal
11 = H2O
12 = Heart
13 = Heart Fetal
14 = Lung
15 = Lung
16 = Lung Tumor
17 = Breast
18 = Breast
19 = Breast Tumor
20 = Muscle
21 = Muscle
22 = Retina
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