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(DMB 01) M.Sc. (Previous) DEGREE EXAMINATION, MAY 2008. First Year Microbiology Paper I — INTRODUCTION TO MICROORGANISMS Time : Three hours Maximum : 100 marks PART A — (5 8 = 40 marks) Answer any FIVE of the following. All questions carry equal marks. 1. Describe the controversy on the doctrine of spontaneous generation or Abiogenesis. 2. Describe the structure of prokaryotic cell with a neat–labeled diagram. 3. Explain the general character and classification of Actinomycetes. 4. Write about the Agrobacterium and its applications. 5. Classification of viruses. 6. Write on the subviral particles. 7. Discuss the economic importance of microalgae. 8. Reproduction in microalgae. PART B — (4 15 = 60 marks) Answer all FOUR questions. All questions carry equal marks. 9. (a) Discuss the ultra structure of a typical bacterial cell. Add note on the microbial diversity. Or (b) Describe germ theory of diseases. Write about the contributions of Louis Pasteur and Robert Koch in the development of vaccines. 10. (a) Describe the principles and major characteristics considered in the bacterial Taxonomy. Or 11. (b) Write the detailed general account of the genera Bacillus and Clostridium. (a) Explain the Ultra structure and replication of TMV. Or (b) Describe the symptoms and the methods of viral transmission. 12. (a) Write the classification, general account of structure, reproduction and significance of protozoa. Or (b) Write an essay on the classification, structure, reproduction and economic importance of Fungi. —————— (DMB 02) M.Sc. (Previous) DEGREE EXAMINATION, MAY 2008. First Year Microbiology Paper II — MICROBIOLOGICAL METHODS Time : Three hours Maximum : 100 marks PART A — (5 × 8 = 40 marks) Answer any FIVE of the following. All questions carry equal marks. 1. Scanning and transmission Electron Microscopy. 2. Describe the composition of general bacteriological media. 3. Write about the enrichment technique. 4. Describe any two methods of anaerobic bacterial culturing. 5. Write the general principle of centrifugation. Explain the density gradient centrifugation. 6. Discuss the principle and application of HPLC. 7. Liquid Scintillation counter, 8. Autoradiography. PART B — (4 × 15 = 60 marks) Answer all FOUR questions. All questions carry equal marks. 9. (a) detail. Describe the different staining methods of bacteria and their advantages in Or (b) Discuss the purpose and importance of sterilization in microbiology. Elaborate the methods of sterilization. 10. (a) Discuss the general methods of bacterial Isolation. Explain the advantages of each method. Or (b) Explain the need and necessity for the maintenance and preservation of microbial cultures. 11. (a) Write an essay on the various methods used in the cultivation of viruses. Or 12. (b) Write in detail about the Isolation and purification of viruses. (a) Describe the general principle of Electrophoresis and its applications. Or (b) Describe spectrophotometer. the principle, construction and application of UV – Vis —————— (DMB 03) M.Sc. (Previous) DEGREE EXAMINATION, MAY 2008. First Year Microbiology Paper III — MICROBIAL PHYSIOLOGY AND BIOCHEMISTRY Time : Three hours Maximum : 100 marks SECTION A — (5 8 = 40 marks) Answer any FIVE of the following. All questions carry equal marks. 1. Essential macronutrients. 2. Synchronous cultures. 3. Nitrate oxidizers. 4. Iron oxidizers. 5. HMP Pathway. 6. Oxidative phosphorylation. 7. Iso enzymes. 8. Types and functions of RNAS. SECTION B — (4 15 = 60 marks) Answer all the FOUR questions. All questions carry equal marks. 9. (a) Discuss the mechanisms of transport of nutrients in bacteria. Or 10. (b) How physical and chemical factors affect bacterial growth? (a) Discuss the characteristics of different groups of phototrophic bacteria. Or (b) Compare and contrast between anoxygenic and oxygenic phototrophic bacteria. 11. (a) Write on arrangement of electron carriers in bacteria. Or 12. (b) Discuss the diversity of respiratory pathways in bacteria. (a) How different factors influence the enzymatic activity in bacteria? Or (b) Write the structures and functions of different nucleotides. ——————— (DMB 04) M.Sc. (Previous) DEGREE EXAMINATION, MAY 2008. First Year Microbiology Paper IV — ENVIRONMENTAL AND AGRICULTURAL MICROBIOLOGY Time : Three hours Maximum : 100 marks SECTION A — (5 8 = 40 marks) Answer any FIVE of the following. All questions carry equal marks. 1. Aeroauergens. 2. Coliform test. 3. Tranformation of sulphur. 4. Rhizosphere. 5. Plant growth promoting bacteria. 6. VAM fungi. 7. Powdery mildew of cucurbits. 8. Biological control of plant diseases. SECTION B — (4 15 = 60 marks) Answer all the FOUR questions. All questions carry equal marks. 9. (a) What are the diseases caused by common water – borne pathogens? How do you control them? Or 10. (b) Discuss the relevance of aerobiology to plant pathology. (a) Explain the commonly used methods for the isolation of different types of soil microogranisms. Or 11. (b) Discuss the decomposition of soil organic matter. (a) Explain the nitrogen fixation by free living soil microogranisms. Or 12. (b) Describe the structure and functioning of leguma root nodules. (a) Explain the symptoms caused by plant pathogenic fungi with examples. Or (b) Discuss the cultural and chemical control practices generally followed for the management of plant diseases. ——————