Download Zebrafish Monoclonal Antibodies

Zebrafish Monoclonal Antibodies
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(Source: K. Larison and B. Trevarrow from Zebrafish Book 5th Edition)
Monoclonal antibodies that recognize a variety of structures in zebrafish embryos are available from the library at the Zebrafish International
Resource Center. Antibodies can be ordered online at: http://zebrafish.org/. Please feel free to communicate with ZIRC if you have any questions:
[email protected]
The following is a partial list of monoclonal antibodies raised against zebrafish immunogens at the University of Oregon. These are available from
the Zebrafish International Resource Center (http://zebrafish.org/).
Antibody
Name
Isotype
Cellular Binding
Site
anti-DLX3b IgG1,k
Anatomical Structure Labeled
border of neural plate, otic placode, otic vesicle, olfactory epithelium, median finfold,
anti-VegT
IgG2a,k
zl-1
IgG1,k
cytoplasm or
extracellular
matrix
adaxial cells, lateral mesoderm, prechordal plate, tail bud
lens fiber cells
zm-1
IgG1,k
granular
cytoplasm
actinotrichia, pectoral fins, ventral fin fold, heart, musculature system, branchial muscles, enteric musculature, extraocular musculature,
abdominal musculature
zm-2
IgG1,k
granular
cytoplasm
actinotrichia, meninx primitiva, dorsal fin musculature
zm-3
IgG1,k
granular
cytoplasm
musculature system, branchial muscles, enteric musculature, extraocular musculature, myotomes, somites,
zm-4
IgG1,k
zn-1
IgG1,k
cytoplasm
axons, blastomeres, dorsal root ganglia, enteric nervous system, sensory hair cells, neurons, primary motoneurons, peripheral nervous
system, Rohon-Beard neurons, YSL
zn-2
cell surface
ventral root, forerunner cell group, sensory hair cells, epibranchial placodes, lens, brain (neuropil), Rohon-Beard neurons, dorsal roots,
trigeminal ganglion, trigeminal nerve (cranial nerve V), organs
zn-3
IgG2b,k cell surface
cranial ganglia, meninx primitiva, enteric nervous system, pectoral fins, rhombomeres, retinal inner plexiform layer, neurons, brain
(neuropil), retinal outer plexiform layer, peripheral nervous system, neurites
zn-4
IgG1,k
cytoplasm or
cell surface
retinal neural layer, fins, retinal ganglion cell layer, sensory hair cells, rhombomeres, retinal inner plexiform layer, neural crest cells,
neurons, brain (neuropil), epiphysis, neurites, neuromeres, gut epithelium
zn-5
IgG1,k
cell surface
subset of CNS tracts and commissures, retinal ganglion cell layer, epibranchial placodes, muscle pioneers, neural crest cells, brain
(neuropil), ganglia, Rohon-Beard neurons, ventral spinal cord neurons
zn-6
IgG1,k
cell surface
dorsal spinal cord neurons, enteric nervous system, retinal ganglion cell layer, retinal inner plexiform layer, musculature system, brain
(neuropil), retinal neural layer, retinal outer plexiform layer, peripheral nervous system, brain stem/spinal tracts and commissures
zn-7
IgG1
cytoplasm
brain, gut, sensory hair cells, statoacoustic (VIII) ganglion, neural crest cells, retinal neural layer, epiphysis, posterior lateral line ganglion,
posterior lateral line nerve, lateral spinal cord neurons, spinal cord
zn-8
IgG1,k
cell surface
subset of brain (cellular), epibranchial placodes, horizontal myoseptum, Rohon-Beard neurons, somites, trunk mesenchyme, ventral spinal
cord neurons
zn-9
IgG1,k
zn-10
IgG1
zn-11
IgG2b
zn-12
IgG1,k
cell surface
cranial ganglia, dorsal spinal cord neurons, enteric nervous system, dorsal fin, ventral fin, epibranchial placodes, musculature system,
myotome, retinal neural layer, Rohon-Beard neurons
zn-13
IgM
cell surface
dorsal spinal cord neurons, enteric nervous system, sensory hair cells, musculature system, myotomes, neuropil, Rohon-Beard neurons,
trigeminal ganglion, trigeminal nerve (cranial nerve V)
zn-14
IgM
cell surface
sensory hair cells, rhombomeres, retinal inner plexiform layer, neural crest cells, brain (neuropil and cellular), retinal outer plexiform layer,
peripheral nervous system, unidentified cell types on spinal cord surface
zn-15
IgG1,k
cytoplasm or
brain vasculature, central nervous system, enteric nervous system, sensory hair cells, rhombomeres, retinal inner plexiform layer, neurons,
brain (neuropil), retinal outer plexiform layer, epiphysis, posterior lateral line nerve, peripheral nervous system, neurites
cell surface
enteric nervous system, fins, hindbrain (hindbrain neuropil), fourth ventricle, retinal outer plexiform layer, peripheral nervous system, lateral
spinal cord neurons, trigeminal ganglion, trigeminal nerve (cranial nerve V)
dorsal root ganglion, enteric nervous system, sensory hair cells, retinal inner plexiform layer, neurons, brain (neuropil), retinal neural layer,
peripheral nervous system, YSL
dorsal root ganglion, EVL, retinal ganglion cell layer, sensory hair cells, rhombomeres, neurons, brain (neuropil), epiphysis, ganglia
cell surface
zna-1
IgG1,k
rhombomeres, brain (neuropil), cell somata, growth cones
znp-1
IgG2a,k cell surface
rhombomeres, retinal inner plexiform layer, brain (neuropil), subset of spinal cord (cellular), ventral root, YSL
znp-4
IgG1
retinal inner plexiform layer, brain (neuropil), retinal outer plexiform layer, spinal cord
znp-5
IgG1
znp-6
IgG1,k
cell surface
cell surface
brain (neuropil), punctate label in medial longitudinal fasciculus, brain (neuropil), lateral spinal cord neurons, spinal cord ventral neurons
central nervous system, EVL, gills, lens, myotomes, brain (neuropil), retinal neural layer, lateral spinal cord neurons, somites, spinal cord
ventral neurons, visceral organs, YSL
znp-7
IgG1,k
cell surface
zns-1
IgG1,k
zns-2
IgG1,k
cell surface
brain (neuropil), enteric nervous system, sensory hair cells, hindbrain radial glial cell, retinal inner plexiform layer, olfactory placode, retinal
outer plexiform layer, actinotrichia
zns-5
IgG1,k
cell surface
cranial ganglia, central nervous system, enteric nervous system, fins, sensory hair cells, rhombomeres, retinal inner plexiform layer, retinal
outer plexiform layer, inner ear, peripheral nervous system
zns-6
IgG1,k
zns-7
IgG1,k
cytoplasm
central nervous system, DEL cells, oral epithelium, EVL, gut, horizontal myoseptum, lens, myotomes, brain (neuropil), notochord, somites,
YSL
znt-1
IgM
cytoplasm or
extracellular
matrix
actinotrichia of median finfold, neural crest cells, retina, apical ectodermal ridge of pectoral fin, sensory hair cells, retinal inner plexiform
layer, lens, myotome, brain (neuropil), notochord, subset of inner ear
znu-2
IgG1,k
zpr-1
IgG1,k
zpr-2
IgG1,k
zpr-3
IgG1,k
zrf-1
IgG1
zrf-2
IgG1,k
zrf-3
IgG1,k
cell surface
unidentified structure close to meninx primitiva, radial glial cells in rhombomere , retinal inner plexiform layer, retinal outer plexiform layer,
posterior lateral line nerve, radial glial cells, lateral spinal cord neurons
zrf-4
IgG1,k
cell surface
central nervous system, radial glial cells in rhombomeres, optic nerve (cranial nerve II), radial glial cells
zs-3
IgG1,k
cytoplasm
retinal neural layer
zs-4
IgG1,k
cytoplasm
central nervous system, DEL cells, EVL, lens, lens epithelium, musculature system, YSL
cytoplasm
cell surface
brain (neuropil), sensory hair cells, rhombomeres, retinal inner plexiform layer, subset of retinal neural layer and spinal cord (cellular), brain
(neuropil), retinal outer plexiform layer, lateral spinal cord neurons, spinal cord ventral neurons, YSL
,
central nervous system, enteric nervous system, ciliary marginal zone, rhombomere, retinal inner plexiform layer, retinal outer plexiform
layer, peripheral nervous system
central nervous system, ectoderm, rhombomeres, retinal inner plexiform layer, myotomes, brain (neuropil), retinal outer plexiform layer,
radial glial cells
subset of brain (cellular), DEL cells, EVL, lens, retinal neural layer, Rohon-Beard neurons
cytoplasm
cytoplasm
cytoplasm
cytoplasm
cytoplasm
retinal photoreceptor cell layer, epiphysis
retinal photoreceptor cell layer, epiphysis
retinal photoreceptor layer, photoreceptor outer segment layer
dorsoventral diencephalic tract, gut epithelium, Muller cells, notochord, radial glial cells in rhombomeres and spinal cord, ventral roots
radial glial cells