Download Ventilation in the Laboratory

Biological Safety
BIOSAFETY LEVEL 2 AWARENESS TRAINING
UW Stout Laboratory Safety
Introduction
 This training provides a basic understanding of
biosafety hazards and practices required to enter
UW-Stout’s designated biosafety level 2 (BSL-2)
laboratory spaces
 It does not provide all required training (e.g.,
bloodborne pathogens, use of biosafety cabinets,
standard microbiological practices) for work with
agents in BSL-2 containment
 It is a supplement to, not a replacement for, the
Laboratory Safety Training modules on D2L
What Does “Biosafety Level 2” Mean?
 The biosafety level (BSL) defines the laboratory
practices and techniques, safety equipment and
laboratory facilities recommended for work with
biohazardous materials. UW-Stout has facilities for
working with materials that require BSL-1 or BSL-2
containment:


BSL-1: For work with defined and characterized strains of
viable microorganisms not known to consistently cause
disease in healthy adult humans
BSL-2: For work with moderate-risk agents that are
associated with human disease of varying severity
What are “Biohazardous Materials”?
 Biohazardous materials are infectious agents or
hazardous biological materials that present a
potential risk to the health of humans, animals or the
environment. They include:



Recombinant DNA
Organisms that can affect health (e.g., parasites, bacteria,
fungi, viruses)
Biologically active agents (e.g., toxins, allergens, venoms)
How Do I Know Which are BSL-2 Labs?
 All doors to laboratory or
classroom spaces that
contain materials being
worked with using BSL-2
criteria will have the sign
shown on the right
 The sign advises of entry
and exit requirements that
must be followed by
everyone who enters the
space, not just people
working with the materials
 Current BSL-2 Labs in
Jarvis are 247, 249, 257
and 267
Can I Do Other Work in a BSL-2 Lab?
 Multi-use labs may contain areas that are not to be used
for BSL-2 work; these areas are designated with blue
washable tape placed along the perimeter of the work
surfaces
 These blue-labeled areas must not be used for BSL-2
work, and will not require decontamination specific to
BSL-2 areas
 Equipment from blue-labeled areas can be moved to and
from non-BSL-2 areas without decontamination
 Everyone entering the lab should familiarize themselves
with the location of the non-BSL-2 spaces
What are the Hazards?
 Biohazardous materials that require BSL-2 containment
have a moderate risk of causing human disease
 Exposure could occur from:



Accidental needle stick or other skin puncture
Splash into eyes, nose, mouth
Accidental ingestion
The risk of being exposed by being in the same room is extremely low.
 If handled carefully (using standard microbiological
practices) these materials can be used safely on open
benches

Procedures that may create infectious aerosols or splashes should be
performed in a biological safety cabinet
What are the Rules?
 Lab coats are mandatory
 No open-toed shoes
 Safety glasses must be worn by everyone if someone in the lab




is working with a liquid chemical or biohazardous material
Chemical splash goggles (indirectly vented or un-vented; NOT
vented impact goggles) must be worn by people working with
a biohazardous material if there is a possibility of creating a
splash
Lab doors must not be propped open
Eating, drinking, smoking, handling contact lenses, applying
cosmetics and storing food for human consumption are not
allowed at any time
Hands must be washed with soap and water before leaving the
room
What Do I Do With My Lab Coat?
 Lab coats should always be left in the lab
 Lab coats will be laundered for you at the end of each
semester, at a minimum
 Do not take personal lab coats home until after they
have been laundered (talk to your instructor or lab
manager for confirmation)
 If your lab coat is visibly contaminated with
biological materials, it should be autoclaved and
laundered immediately
Conclusion
 This concludes the BSL-2 Awareness Training
Module
 Please complete the BSL-2 Entry Quiz Requirement,
Quiz 12 – BSL-2 Entry