Download DNA Mutations WS Practice

Name ________________________________ Per. _____ Date _______________________
Biology
DNA Replication, Transcription, Translation, and Mutation
Cystic Fibrosis Gene: When both copies of this gene are mutant, individuals have lung
and kidney problems.
1. You will be able to write DNA sequence for the complementary strand.
2. You will use the complementary strand to write the mRNA.
3. You will be able to write the protein sequence.
A. Normal Gene (a small portion only)
5'- ATG ATT AAA GAA AAT ATC ATC TTT GGT GTT TCC TAT GAT - 3'
1.
__________________________________________________________________________
2.
__________________________________________________________________________
3.
_________________________________________________________________________
B. Known Mutant Gene (Deletion)
5' - ATG ATT AAA GAA AAT ATC ATC --- GGT GTT TCC TAT GAT - 3'
1.
_________________________________________________________________________
2.
__________________________________________________________________________
3.
_________________________________________________________________________
C. Hypothetical Mutant Gene 1 (Substitution)
5' - ATG ATT AAA GAA AAA ATC ATC TTT GGT GTT TCC TAT GAT- 3'
1.
_________________________________________________________________________
2.
__________________________________________________________________________
3.
_________________________________________________________________________
D. Hypothetical Mutant Gene 2 (Frameshift)
5' - ATG ATT AAA GAA CAA TAT CAT CTT TGG TGT TTC CTA TGA T - 3'
1.
_________________________________________________________________________
2.
__________________________________________________________________________
3.
_________________________________________________________________________
Name ________________________________ Per. _____ Date _______________________
Mutation - a change in the DNA
Point mutations - when a single nitrogen base is deleted, inserted or substituted.
Frameshift Mutation- when many amino acids coded for are different because of
an insertion or deletion.
The following example shows how these 3 kinds of mutations work. Fill out the mRNA
and protein for each and compare the mutations to the original. The mutation is
underlined.
Original DNA T A C G C G T G C A C G A T G C A G T A G T A C
mRNA _____________________________________________________________________
Protein _____________________________________________________________________
Mutation 1 DNA T A C G C G T G C A C G A T C C A G T A G T A C
mRNA _____________________________________________________________________
Protein _____________________________________________________________________
Type of Mutation: _____________________________________________________________
Mutation 2 DNA T A C G C G T G C T C G A T G C A G T A G T A C
mRNA _____________________________________________________________________
Protein _____________________________________________________________________
Type of Mutation: _____________________________________________________________
Mutation 3 DNA T A C G C G C T G C A C G A T G C A G T A G T A C
mRNA _____________________________________________________________________
Protein _____________________________________________________________________
Type of Mutation: ____________________________________________________________