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Abstract
Salvia officinalis (SO) and Thymus vulgaris (TV) are medicinal plants well known for
their curative powers. However, the molecular mechanisms responsible for these
abilities of sage and thyme have not been fully understood yet. In this study we
investigated the composition and the quantitative estimation of plant extracts, the
protective effects of plant extracts against hydrogen peroxide- and 2,3-dimethoxy1,4-naphthoquinone-induced DNA damage, and levels of enzymatic and nonenzymatic antioxidants (superoxide dismutase, glutathione peroxidase, glutathione)
in human HepG2 cells. To measure antioxidative activity of plant extracts we used
three assays: 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant
power (FRAP) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The
results showed that the oxidant-induced DNA lesions were significantly reduced in
cells pre-treated with the plant extracts studied. The observed DNA-protective
activity could be explained by both elevation of GPx activity in cells pre-treated with
SO and TV and antioxidant activity of SO and TV.
Keywords
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Salvia officinalis extract;
Thymus vulgaris extract;
Antioxidant activity;
Human hepatoma HepG2 cells;
Comet assay;
Antioxidant enzymes
Corresponding author. Address: Laboratory of Mutagenesis and Carcinogenesis, Cancer
Research Institute, Slovak Academy of Sciences, Vlárska 7, 833 91 Bratislava, Slovak
Republic. Tel.: +421 2 59327 526; fax: +421 2 59327 506.
Copyright © 2013 Elsevier Ltd. All rights reserved.
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