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NEW TEST ANNOUNCEMENT 1-800-533-1710 NOTIFICATION DATE: April 11, 2014 EFFECTIVE DATE: May 7, 2014 Herpes Simplex Virus (HSV), Culture from Neonates VHSV USEFUL FOR: An aid in the diagnosis of congenital herpes simplex virus (HSV) infection through the recovery of HSV using viral culture (shell-vial) on neonates (< 35 days old). METHODOLOGY: Virus culture by shell-vial REFERENCE VALUES: No virus isolated SPECIMEN REQUIREMENTS: Specimen Type: Swab Container/Tube: Dacron-tipped swab with plastic handle (Supply T507) and M5 media Sources: ocular, rectal, anus, skin, dermal, mouth, nasopharynx, conjunctiva, eye Collection Instructions: send entire swab in M5 viral transport media Specimen Type: Urine Container/Tube: Sterile container Specimen Volume: 1 mL Specimen Minimum Volume: 0.5 mL Specimen Type: Stool Container/Tube: Sterile container Specimen Volume: 5 to 10 g Specimen Minimum Volume: 5 g SPECIMEN STABILITY INFORMATION: Specimen Type Varies Temperature Refrigerated Time 7 days CAUTIONS: This test should not be performed on patients older than 35 days of age. It is intended for the recovery of herpes simplex virus (HSV) in suspected cases of congenital herpes. For patients older than 35 days of age, detection of HSV by real-time PCR is recommended. Real-time PCR for HSV is the recommended test for all cases of central nervous system disease caused by this virus. Viral culture is an insensitive approach for detection of HSV in cerebrospinal fluid. CPT CODE: 87254 X 2 DAY(S) SET UP: Monday through Sunday ANALYTIC TIME: 14 days QUESTIONS: Contact your Mayo Medical Laboratories’ Regional Manager or Kim J. Baker, MML Laboratory Technologist Resource Coordinator Telephone: 800-533-1710