Download Amino Acids LC-MS/MS Analysis Kit

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

page
1
page
2
Document related concepts
no text concepts found
Transcript 
Amino Acids
LC-MS/MS Analysis Kit
METABOLIC DISEASES
in Biological Fluids
Accurate, sensitive
rapid and quantitative
analysis of
42 free amino acids
in a single run
within 20 minutes
Distribuido en España por:
INGENIERIA ANALITICA S.L.
Tel: (+34) 902.45.6677
Fax: (+34) 902.46.6677
www.ingenieria-analitica.com
[email protected]
Suitable for any
LC-MS/MS System
Main methods and procedures that have been
selected are based on EN ISO 13485 and 98/79/EC.
Properties of the analysis kit
20 minutes analysis time
Available in Biological Fluids:
Plasma, Serum, Urine, Cerebrospinal Fluid
Sensitive separation with Zivak® AA HPLC column
Deuterated internal standards
Approximately 2000 analysis with one column
ZIVAK TECHNOLOGIES Eski Besiktas Cad. No 44, 34400 ISTANBUL - TURKEY
+90 212 210 62 15
+90 212 210 62 14
[email protected]
www.zivak.com
Amino Acids
LC-MS/MS Analysis Kit
METABOLIC DISEASES
in Biological Fluids
Validation Results
LOD
No
Analyte
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
3-methyl-histidine (3-MeHIS)
Alanine (ALA)
Alpha-aminoadipic acid (AAA)
Alpha-aminobutyric acid (ABA)
Alpha-aminopimelic acid (APA)
Anserine ( ANS)
Arginine (ARG)
Argininosuccinic acid (ARG-SUC)
Asparagine (ASN)
Aspartic Acid (ASP)
Beta alanine (BALA)
Beta-aminoisobutyric acid (BAIB)
Citrulline (CIT)
Cystathionine (CTH)
Cysteine (CYS)
Cystine (C-C)
Gamma-aminobutyric acid (GABA)
Glutamic Acid (GLU)
Glutamine (GLN)
Glycine (GLY)
Histamine (HISTA)
Histidine (HIS)
Hydroxylysine (HYL)
Hydroxyproline (HYP)
Homocystine (HC-HC)
Homocysteine (HCYS)
Isoleucine (ILEU)
Leucine (LEU)
Lysine (LYS)
Methionine (MET)
1-methyl-histidine (1-MeHIS)
Ornithine (ORN)
Phenylalanine (PHE)
Proline (PRO)
Sarcosine (SAR)
Serine (SER)
Serotonin (SRTN)
Thiaproline (THPR)
Threonine (THR)
Tryptophan (TRP)
Tyrosine (TYR)
Valine (VAL)
(µmol/L)
LOQ
Accuracy
Precision
Precision
Linearity
6.57
2.57
4.95
2.74
3.96
5.45
4.13
6.93
2.87
2.18
2.18
7.69
3.86
7.66
2.74
0.86
10.9
4.39
3.27
5.45
2.74
7.59
2.18
6.93
0.95
0.58
2.74
4.13
1.09
6.40
2.74
6.40
1.09
2.87
4.16
1.98
4.39
6.90
2.57
2.15
2.81
3.14
90.9
91.7
95.1
96.3
97.4
95.6
91.7
94.6
89.8
94.5
96.2
95.6
94.3
96.4
92.2
97.5
94.6
92.8
93.6
96.4
91.5
96.3
98.2
94.8
96.7
94.8
93.3
95.6
91.3
92.5
93.6
91.8
92.6
95.6
90.7
95.5
97.2
91.9
90.6
92.7
88.7
89.8
4.23
4.85
5.21
5.01
4.76
6.47
4.25
4.16
4.12
5.14
5.28
4.75
6.84
3.45
4.72
4.53
4.21
5.42
4.13
4.57
4.26
4.75
6.14
4.56
4.12
4.78
5.01
4.86
4.12
4.76
6.76
5.43
3.56
4.25
5.47
3.56
4.72
4.56
5.26
3.57
2.59
3.04
5.1
5.1
6.3
5.3
5.4
7.4
5.1
5.3
5.0
6.2
5.4
5.3
7.2
4.4
5.1
5.2
5.3
6.4
5.2
5.4
5.1
5.4
7.7
5.3
5.2
5.5
5.2
5.3
5.1
5.1
7.2
6.4
4.2
5.3
6.2
4.4
5.5
5.7
6.0
4.7
3.8
3.2
0.994
0.996
0.994
0.997
0.985
0.994
0.984
0.993
0.997
0.996
0.985
0.994
0.984
0.997
0.993
0.997
0.995
0.994
0.998
0.989
0.986
0.994
0.997
0.993
0.985
0.999
0.996
0.987
0.994
0.997
0.993
0.994
0.994
0.996
0.985
0.994
0.991
0.992
0.994
0.996
0.993
0.997
(µmol/L)
1.99
0.78
1.50
0.83
1.20
1.65
1.25
2.10
0.87
0.66
0.66
2.33
1.17
2.32
0.83
0.35
3.30
1.33
0.99
1.65
0.83
2.30
0.66
2.10
0.32
0.25
0.83
1.25
0.33
1.94
0.83
1.94
0.33
0.87
1.26
0.60
1.33
2.09
0.78
0.65
0.85
0.95
(%)
Intra-Assay
(%CV)
Inter-Assay
(%CV)
The test results have been validated with an AB Sciex API 4000 LC-MS/MS instrument in Nov. 2014.
ZIVAK TECHNOLOGIES Eski Besiktas Cad. No 44, 34400 ISTANBUL - TURKEY
+90 212 210 62 15
+90 212 210 62 14
[email protected]
www.zivak.com
(R2)
Related documents