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Klug, Old Activity 8.3, Three Point Mapping Klug, NEW Activity 8.3 Three Point Mapping * In its revised format include Parts 8.3.1 through 8.3.12. These parts should be separated from the original tutorial then combined to form this new tutorial, Activity 8.3. It will have it's own Introduction, Summary, and Post-quiz. * Remove the screen that shows on selecting “module quiz” asking if the user wishes to take the quiz. New organization: Activity 8.3, Three Point Mapping Introduction (Part 8.3.1.1) -This will not be programmed as part of the Flash animation. -This will be converted into a single html page with scrolling text. It will use the image from old 8.3.1.1 and text from the first three text screens, but together with existing paragraph breaks. - Delete the last line of the last paragraph saying “Click the Play button…” - The text will scroll up until the last sentence gets to the middle of the text window, where it will pause for a beat, and fade out (with the image) to begin the next segment. -This is the end of the segment. Animation (Part 8.3.2.1) Mapping in Drosophila -Change the title (“Drosophila Example”) that sits over the image to say “Mapping in Drosophila” -Delete all stops between text changes and associated animations -Put the first two text scenes (old 8.2.1-8.2.2) together as one long unit of scrolling text, with existing paragraph breaks. - Delete text of old 8.2.3 saying “ Click the Play button ….” - - The text will scroll up until the last sentence gets to the middle of the text window, where it will pause for a beat, and fade out (with the image) to immediately begin the next segment. - -This is the end of this segment. Exercise (Part 8.3.3) Drosophila Example -This is self-contained. - The instructions in the Usage box should instead pop up from the lower left footer entitled Instructions. Delete the Usage Box button. - Delete the Textbook reference button and pop up interaction. - Use the same text, images, and interactivity as in the existing old 8.2.3.1 which is a table where you drag boxes from the right into the Female Gametes column - Delete the “Alert!” box that appears if the user misses the question more than three times. - The popup comments that appear when the user correctly places the drag boxes should appear in green Correct popup windows - Delete Textbook reference button from the later section of this activity as well - Cause the contents of the Instructions box to change after the user correctly completes the first part of this exercise. The user should get a green border CORRECT box with the “That concludes this part of the exercise…..” and a Continue button. This is where the animation subsequently fills in the Male Gametes when the continue button is pressed. The Instructions box should change now to hold the new Usage instructions that currently pop up (“Now we must enumerate….”) - Delete the Usage pop up box - Delete the “Alert!” box that appears if the user misses the question in the second part of the exercise more than three times. - This is the end of this segment Exercise (Part 8.3.4) Drosophila Example: Phenotypes -This is self-contained. - Delete usage instructions in the last paragraph of the text (“STEP 1: Identify…..”) and instead pop up these from the Instructions box with ALL the step instructions for the exercise from the lower left footer entitled Instructions. The instructions should read: STEP 1: Identify the noncrossover classes by selecting the corresponding numbers from the checkboxes at the bottom and clicking on Submit Answer 1. STEP 2: Next, identify the reciprocal classes of double crossovers. Recall that they generally have far fewer crossover events than the noncrossover or single crossover classes. Select the numbers in the checkboxes below the table that correspond to the double crossover classes and click the Submit Answer 2 button. STEP 3: Now locate one of the two sets of single crossover classes. First, identify the classes corresponding to a single crossover between y and w. As before, select the appropriate number buttons and click on Submit Answer 3. STEP 4: Finally, identify the classes corresponding to a single crossover event between w and ec. Select the appropriate numbers with the buttons below the table and click Submit Answer 4. - - The instructions text should be able to pop up in the old text space and encompass all of the instructions, Steps 1-4. The introductory text for this exercise (“The adjacent table …”) can remain in the text space but get covered up by pop-up boxes if necessary. -Replace the Submit Button with four buttons entitled: Submit Answer 1, Submit Answer 2, Submit Answer 3, Submit Answer 4. The last sentence of the first paragraph of introductory text accompanying the exercise (“We shall now use this…”) should be modifed to read” We shall now use this data to map the relative positions of the y, w, and ec loci on the chromosome”. Move any positive feedback text (“That is correct…..”) from the old text area and pop it up in a green header Correct boxes when the answers submitted are correct. - Move any negative feedback text to a red header Incorrect popup box. Exercise (Part 8.3.5) Drosophila Example: Events -This is self-contained. - Move instructions (the whole text section) to an Interaction Instructions popup from a left footer - Change the last line of the text , second paragraph,to read “when you are finished entering and checking these four numbers, click the Submit Answer button”. - Move Submit and Reset buttons one above the other, and change the Submit button to “Submit Answer”. - Delete the Textbook reference button and pop up interaction. - Use the same text, images, and interactivity as in the existing old 8.3.5, the first screen of Drosophila Example: Events. - Cause the contents of the Instructions box to change after the user correctly completes all the boxes with the right numbers. The new Instructions text should be the excised last paragraph that appears after the first part is completed:” Using the table on the left, fill in….”. - Put the Wrong Answer feedback into a red header Incorrect Box. - There is sometimes an error in the image display (the table) in that there is no entry box for the user to type in a number for the 3rd row in the table. Unless the user can enter a number, the programming will never mark the other answers correct. Add an entry box in the 3rd row, last column headed “Single crossover between w and ec. Include the percentage calculation under the entry box, based on what the user enters. - Continue to offer the special Incorrect Box which gives the correct answers after the question is missed three times. - Keep the final “That is correct! With our table complete….” text that appears after filling in the right numbers into the table, but remove the instructions (“Using the table on the left”) to the Instructions box as already specified. Modify the last sentence of these instructions to say “ …you have entered the correct values, click the Submit Answer button”. - Change the Submit button in 8.3.5.3 to a Submit Answer button - After the second part of the exercise where the user correctly enters the map units into the two boxes (8.3.5.3), modify the second paragraph of the text that appears (“Click the Play button to jump to a summary….) to instead say (“We will now move to a summary of the three-point mapping problem that you have just solved”). - Dissolve from the completion of 8.3.5.3 (putting in map units) after a few beats (allow reading time) to the summary completed table on New 8.3.5.4. - This is the end of the segment. - Exercise (Part 8.3.6) Gene Order: Introduction - This segment begins (8.3.6.1) with text that scrolls slowly from the bottom to the top of the page (“In the mapping example that we…”) but modify it to read line of the paragraph (“Click the Play button…..”) is eliminated. - When the text reaches the top of the text column, freeze, wait a beat, and dissolve both the text and the animation into 8.3.6.2. Eliminate the stop. - The new 8.3.8.2 features the modified text “Let’s illustrate how to find the gene order for the preceding mapping example. In the illustrations at the left, we show double crossover diagrams for the three possible orders of the genes (w-y-ec, y-ec-w, and y-w-ec). Below them are the six doublecrossover gametes that could result.” - Allow the DRAG arrow to disappear as it now does when the first item is dragged. - In the Instructions box, make it read:” The illustrations show double crossover diagrams for the three possible orders of three genes (w-y-ec, yec-w, and y-w-ec). Below them are the six double crossover gametes that could result. Drag the sets of double crossover gametes that could result from these situations to the appropriate position.” - Move the That is Correct feedback to a green header Correct box - This is the end of this segment. Exercise (Part 8.3.7) Gene Order:Phenotypes - Use the interactivity from old 8.3.7 - Keep the text but change the last line to “When you are happy with your selections, click the Submit Answers button”. - Move positive and negative feedback popups into the appropriate Correct and Incorrect feedback popups. - Retain checkmarks for correct (green) and X marks (red) to show right from wrong upon submission of answers. - Keep the reset button. - Place the reference image from 8.3.1 under the text lists the letter abbreviations and what they equal – just copy the yellow box from 8.3.1 that says “y= yellow body (recessive), y+= dark body (wild type)……" - Move the current instructions under the image that say “Make a choice for each….” to a popup box from the Interaction Instructions footer - Change the Score button to a Submit Answers button Animation (Part 8.3.8) Three Point Mapping Example: Maize (Maize example:introduction) - Keep the same image and paste together the four screens of text (old 8.3.8.1-4) into a single scrolling text unit, with paragraph breaks. Delete the very last line about “Click the Play…” - When last line of the the combined text nears the top of the text window, freeze it, and after a beat, dissolve to old 8.3.8.5 text “What is the initial heterozygous allele….” with accompanying image. - Delete the last sentence of the text “Click the Play button…”. - This is the end of the segment. Exercise (Part 8.3.9) Double Crossovers in Maize (Old Maize Example, Double Crossovers - Change the title of this exercise to Double Crossovers in Maize - Set up an Interaction Instructions footer that pops up: “Drag the boxes showing allelic combinations (at the bottom) to the appropriate crossover diagram that would lead to a particular combination.” - Use the same interactivity as exists for old 8.3.9. - Retain a green Correct popup box with positive feedback and a Continue button. - When the user clicks on CONTINUE, dissolve to 8.3.9.2 featuring the text saying “The correct gene order is…” but delete the last sentence saying “Select the correct order by…..” - Use the same interactivity as exists in 8.3.9.2 - Set up an Interaction Instructions footer that pops up: “Select the correct order by clicking the appropriate checkbox to the left of the diagrams below and then clicking Submit Answer..” - Change the Submit button to Submit Answer - Move all negative feedback to a red header Incorrect popup. - Provide a Correct feedback popup, in addition to the highlighting that already occurs, when the correct answer is selected. The box should contain the deleted text that appears in the text area which says, “That is correct. The only gene order that is consistent with the double crossover phenotypes that are observed is with pr in the middle. From this point onward, we take the gene order to be v-pr-bm.” - Delete any reference to Click the Play button. - Include a CONTINUE button in the Correct feedback popup to dissolve to the next screen, 8.3.9.3 with the text “Since we have determined….” and the accompanying image. - Allow the 8.3.9.3 text with embedded pictures to sit for 5 seconds and dissolve text to 8.3.9.4 which says “Now we have all the information that we need to construct the chromosome map. The map distance between any two genes is the sum of the percentages of all detectable recominational events between them. This includes both single and double crossover events involving the two genes in question.” Delete the last sentence “Using the percentages….”. - The Interaction Instructions button, when clicked, should now say: “Using the percentages in the table, enter the v-pr and pr-bm distances in map units, and click the Submit Answer button”. - Change the Submit button to a Submit Answer button. - There is an error that allows the correct answers to appear in the “Wrong Answer” feedback box after only a single incorrect attempt at the questions. The red header Incorrect box should appear, saying for the first two attempts, “This is not correct. Remember that the distance between any two genes is the SUM of the percentages of both single and double crossover class percentages.” - On the third erroneous attempt, the Incorrect box should read as it currently does. - On getting the correct answer, the feedback text “That is correct. The final map….” should all appear only in the Correct feedback box. The image showing the distanced written along the chromosome can also be shown in the Correct box instead of changing on the main screen but can stay on the main screen if popup boxes can’t accommodate images. Exercise (8.3.10) Interference and the Coefficient of Coincidence - Use the same image as in old 8.3.10 and unite the three screens of text in old 8.3.10.1 to 8.3.10.3. Scroll the text with breaks between paragraphs. - Delete the “Click the Play button”reference at the end. This is all is New 8.3.10.1. - When the last line of text nears the top of the text column, freeze, pause, and dissolve to Old 8.3.10.4 - Use the interactivity screen from old 8.3.10.4 - Under Interaction Instructions, write “Determine the following quantities and enter them in the fields provided (Note: calcuate all quantities to three decimal places). When you are finished, click the Submit Answer button.” - Change the Submit button to a Submit Answer button. - For a correct answer, move the positive feedback text into the Correct Box; same with negative feedback text to the Incorrect popup box. - Delete any reference to “Click the Play button…” - This is the end of this segment. Summary (part 8.3.11) -This will not be programmed as part of the Flash animation. Post-quiz (Part 8.3.12) -This will not be programmed as part of the Flash animation. - Delete the screen asking is the quiz is wanted; cut to the chase! First Pass Comments from Eric Stavney 10/28/03 1. In 8.3.2, let’s move the title and caption (“Mapping in Drosophila” with “Mapping cross involving the mutant….”) upwards and center it more to separate them from the graphic. There is plenty of white space above the title and the graphic feels too crowded to me. [Andy 10/29: Eric is correct. The screen is too crowded. Move the yellow box down to the lower-right corner of the screen. Delete the text that is immediately below the title. Center the title over the graphic portion of the screen.] 2. We should add a final popup box when the user successfully completes the dragging of chromosomes in the last column of Exercise 8.3.3 to say something like: “That is correct! You have chosen the right phenotypes for each of the crossover classes.” Right now there’s no feeling of completion at the end of the exercise. [Andy 10/29: Please make this correction.] 3. In Exercise 8.3.3, when the user completes the table by selecting the right radio buttons and submitting answers 1-4, the text “That is correct. You have identified the classes corresponding to single crossover…” comes up. Let’s add the following sentence to the end of this paragraph: “Click Next to continue”. Please don’t add a Next button as this is supposed to refer the Next hyperlink already built into the bottom frame. [Andy 10/29: Just to be safe, the extra sentence should read “You have completed this exercise.” I’d rather not refer to a “Next” because it’s very possible that this exercise will be used by itself without a Next link.] 4. In 8.3.8 near the end of the segment, the scrolling text comes to a halt in a weird location and then is replaced by the summary text and diagram. Is there any way we can vertically lengthen the text scrolling field so that we can stop the scrolling of this text unit when the last line of the last paragraph clears the bottom and still see the first line of the paragraph (“This cross is diagrammed…”)? This would make a nice stopping place for the text unit….and we’d need about 4 seconds pause after finishing the scrolling before dissolving to the final graphic of 8.3.8. [Andy 10/29: Please make these corrections.] 5. In 8.3.9.3 when the text says “Since we have determined the gene order to be….” I originally asked for this to automatically dissolve after 5 seconds to the next screen, 8.3.9.4. This isn’t working. Can we put a CONTINUE button at the bottom right (or somewhere) on 8.3.9.3 to allow the user to control moving to the next screen? Five seconds is not enough to read it all, and we’ve provided usercontrolled sequencing in other screens in this segment, so why not here? [Andy 10/29: Yes, a button would be better.] 6. On the Conclusion page, can we add an extra blank line between the title “Conclusion” and the body of the text – this feels a bit crowded. [Andy 10/29: It’s okay as long as it follows the standard format.]