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Below are the Abstracts from students who completed outstanding research projects at the five schools of public heatlh inspection in Canada. They were recipients of the Len Hiebert-Environmental Health Review Award for 2010 from the Environmental Health Foundation of Canada. ___________________________________________________________________ Esther Tong British Columbia Institute of Technology “Will going “green” make you green? Is consumer health at risk due to reusable shopping bags?” A recent study suggests the usage of reusable shopping bags (RSBs) may have the potential to pose a public health risk. Since RSBs are used to handle food they can be considered food contact surfaces and should be sanitized to maintain the safety of the food, especially ready-to-eat (RTE) items. An electronic survey containing questions about RSB use was distributed. The survey was created using Google Docs. Data collected was analyzed using the chi-square test to determine if there were any significant differences in the practices of groups according to gender, education level and those in the field of environmental health versus the general public. It was determined that there were associations between gender and 1) the use of RSBs and 2) cleaning of RSBs. No association was found between the remaining groups and research questions. Seventy-three percent of respondents reported using RSBs and 49% did not clean them. The conclusion of this study was that the behaviour of consumers regarding RSBs must be improved. Health authorities and public health inspectors could play an active role in education efforts once internal policies and recommendations are established. ___________________________________________________________________________________ Brittainy Paige Cape Breton University “Isolation of bacteria found in ground turkey: The search for Salmonella The purpose of this experiment was to explore methods of isolating Salmonella from food. Ground turkey was the food source since 1/3 of North American poultry is contaminated with Salmonella. The steps necessary to isolate bacteria from a food sample and how to identify if it is indeed Salmonella or some other bacteria are outlined. Salmonella was not isolated from the turkey sample but Enterobacter cloacae and Proteus mirabilis were. Although these are not bacteria commonly associated with food borne disease they do possess the ability to cause illness. _________________________________________________________________________________ Kelli Sparks Concordia University “Environmental Fate and Characteristics of Heptachlor and Heptachlor Epoxide” Heptachlor is a broad-spectrum insecticide used to treat crop and soil insects as well as termites and ants. Many countries banned heptachlor in the late 1970s to early 1980s due to environmental stability, persistence and toxicity. Heptachlor is considered a persistent organic pollutant and is quickly converted to heptachlor epoxide, a metabolite that is more toxic and persistent than heptachlor, in the environment by biological sources. Both chemicals are relatively insoluble in water, but have high affinities for soil, lipid tissues and readily partition to the atmosphere. To determine the fate of heptachlor and heptachlor epoxide in a Northern environment where heptachlor was never applied, a level I fugacity model was used. Both heptachlor and heptachlor epoxide are subjected to long-range atmospheric transport and deposition, which can carry these chemicals to Northern latitudes such as Utikuma Lake, the study site. Using appropriate environmental and chemical parameters it was found that both heptachlor and heptachlor epoxide partition strongly into soil and sediments. However, significant concentrations of both chemicals were found in fish, air and water due to the large emission mass used. Heptachlor epoxide is an issue for environments due to its persistence, stability and lipophilic nature. As such, the equilibrium at Utikuma Lake is expected to shift more chemical into the fish from soil and vegetation sources. Ingestion of fish is the most likely way humans would be exposed to heptachlor or heptachlor epoxide from Utikuma Lake. However, exposure could occur through dermal absorption from soil or inhalation of air and aerosols. Outside of the study area, humans are most likely exposed to heptachlor and heptachlor epoxide through inhalation of volatilized chemical or ingestion of contaminated food. There is little data on the effects of heptachlor and heptachlor epoxide on humans, but effects of mammals are vast and range from excitability with acute exposure to tumour production in chronic exposure. ____________________________________________________________________________________ Shelby Wells First Nations University “Environmental risk factors associated with West Nile virus disease in Saskatchewan” West Nile virus (WNv) is a mosquito-transmitted disease that was first isolated in the West Nile district of Uganda in 1937. The first reports of WNv activity in Canada appeared in 2001 and since then WNv has become established in Canada, infecting more than 4,500 people and killing 28 as of 2009. With no vaccine or specific treatments available for treating WNv, it is important to be able to identify the contributing factors to WNv incidence so that precautionary measures may be put in place. Although approximately 80% of those who become infected with WNv are asymptomatic, infections can result in serious adverse health conditions. The Ministry of Health, Saskatchewan (MOH) has monitored WNv activity in humans, wild birds and mosquitoes since 2001. Since 2003, the MOH has conducted mosquito trapping, identification, and virus isolation at various locations, focusing on southern Saskatchewan from June through August. Mosquitoes are identified according to species and processed for WNv infection. Each mosquito pool is identified as positive or negative for WNv using virus isolation. A retrospective descriptive analysis was conducted to determine the location of mosquito traps at the time of virus circulation, rates of positive mosquito pools, the number of reported WNv cases and to collect information regarding selected environmental variables such as temperatures and rainfall. The goal was to identify possible high-risk factors for WNv cases. Environmental data was compared to abundance data for Culex tarsalis mosquitoes collected from mosquito traps in the various eco-regions of Saskatchewan. Temperature, accumulated degree-days and WNv positive mosquitoes were investigated. Analysis was done for clustering of locations of traps with WNv positive Culex tarsalis and human cases. We found an association between human infection risk and both accumulated degree-days and average temperature. There is some correlation between the percent of positive mosquito pools and the number of human cases. Warmer weather, particularly night-time temperatures had the most significant affect on mosquito emergence and developmental rates as well as human outdoor activity. Despite the widespread occurrence of WNv infection in humans in certain years, the relative predictive value of the different types of environmental indicators remains unclear. However, the importance of climate factors is in agreement with a growing number of reports suggesting that real-time climate data can be useful in WNV risk prediction. ____________________________________________________________________________________ Cameron Coulby Ryerson University Failure to properly maintain a vaccine “cold chain” from manufacture to administration can cause loss of vaccine potency (which can reduce herd and individual immunity) and contribute to vaccine wastage (a waste of taxpayer dollars). Many studies, including those conducted in the 1990s in Ontario, have demonstrated that physicians’ offices can be a significant source of breaks in the cold chain. However, no new information has been collected on the utilization of recently published information, from the Public Health Agency of Canada or otherwise. The purpose of this study was to determine whether or not randomly selected physicians’ offices in Toronto were properly storing and handling vaccines in their possession in order to prevent breaks at this step in the cold chain. This project utilized a self-administered questionnaire instrument distributed via mail to assess knowledge and practice. The standard to which the offices were compared was that set out by the Public Health Agency of Canada and the data was analyzed for mean knowledge and practice and compared to older studies from the province to demonstrate differences and similarities, as well as areas where the cold chain could be improved in physicians’ offices. While knowledge and practice showed significant improvement from previous studies, most offices still indicated using domestic frost-free or bar-style refrigerators for storage, contrary to modern guidelines. This project also reinforces the role of the health department and the Public Health Agency of Canada as leaders in cold chain protocol as indicated by physicians. ____________________________________________________________________________________ Photo 1. Shelby Wells(r) receiving the Len-Hiebert-EHR Award from Carmen Buschow, Acting Program Coordinator, ENHS Program, First Nations University. Photo 2. Cameron Colby receiving the Len-Hiebert EHR Award from Professor Marilyn Lee, School of Occupational and Public Health, Ryerson University.