Download Analysis of Human Colon Cancer Tissues Using Synchrotron

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project

Document related concepts
no text concepts found
Transcript 
Analysis of Human Colon Cancer Tissues Using Synchrotron Infrared Radiation
and Focal-Plane-Array Based Fourier-Transform Infrared Microspectroscopy
Chen-Chieh Chan (張振杰), Pei-Yu Huang (黃佩瑜),
Ching-Iue Chen (陳慶曰), and Yao-Chang Lee (李耀昌)
National Synchrotron Radiation Research Center, Hsinchu, Taiwan
Colorectal tissue samples and colon cancer cells were studied employing
synchrotron-radiation-based Fourier-transform infrared microspectroscopy (SR-FT-IMS) and
focal-plane-array Fourier-transform infrared (FPA-FTIR) spectroscopy. Infrared spectra of colon
tissue revealed that stronger absorption at 1240 cm-1 and 1080 cm-1 for PO2- of DNA as well as
1030 cm-1 for glycogen were found due to a large amount of goblet cells and enteroendocrine cells
within normal tissue than that of in the malignant tissue . Furthermore, we utilized membrane
invasion culture system (MICS) for investigating the metastasis ability of colorectal cancer cells,
SW-403, SW-480, SW-1116 and LoVo. The preliminary results showed that colon adenocarcinoma
cell lines SW-403, SW-480 and LoVo were with highly metastatic ability but SW-1116 due to its
nature of clustering growth. We also studied the effects of anticancer drug, -lapachone, for
inhibiting the ability of DNA replication in different dosage.
Keywords: Synchrotron Radiation Fourier-transform infrared microspectroscopy,
Focal-plane-array, human colon cancer tissue, membrane invasion culture system
References:
[1] Shmuel Argov et al., Journal of Biomedical Optics.2002, 7(2), 248–254.
[2] Claus HH et al., Encyclopedia of life science, 2001, 1, 1-2
[3] Chiang J. Li et al., CANCER RESEARCH, 1995; 55, 3712-3715.
Related documents