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Variant - Purple Gene Chromosome TRAIT FlowerColor Color Trait ––Flower Chromosome Gene Variant -white Genetics based on Law of Segregation & Law of Independent Assortment Dominant [R] Recessive [r] Genotype Phenotype Gene Allele Homozygous [RR or rr] Heterozygous [Rr] Variant Trait This Trait is recessive This gene codes so that the individual does not produce any melanin (protein which gives pigment to hair, eyes, skin) 1. What are the Variants for this trait? 2. What is the Genotype for an albino? (use A a) 3. What is the Phenotype for an albino? (use the physical characteristic…) Mark on white board! Pea Plants – Trait of Flower Color Variants: Purple (gene/allele) P White (gene/allele) p Cross a purple flower (homozygous) with a white flower and determine the Genotypic ratio & Phenotypic ratio of the offspring. 1. Identify the genotypes of the parents ◦ Homozygous Purple - ______ ◦ White - ______ 2. Determine the possible gamete combinations for each parent ◦ Purple parent ___ ◦ White parent ___ 3. 4. ___ ___ Use punnett square to place the parents’ possible gene/alleles for each gamete AND Cross the two gamete alleles to determine the genotype of all the offspring One parent’s genotype along the top, one parent ‘s genotype along the side. p p P ? ? P ? ? 5. Determine the Genotypic ratio All four boxes have a genotype of Pp So…. 1/1 or 100% or all are 100% Pp 6. Pp Determine the Phenotypic ratio What color will the offspring show? So…1/1 or 100% or ALL are Purple Flowers 100% Purple Flowers Round Pea Dominant R Wrinkled Pea Recessive r Problem: Cross a Wrinkled pea with a heterozygous round pea. Determine the genotypic & phenotypic Ratio of the offspring. Wrinkled pea crossed with Heterozygous round 1. pp crossed with a Pp 2. Possible gametes “p” and “P” & “p” p p 3. Place in Punnett square P p 4. Determine genotype of offspring 5. Record % of genotype of offspring 6. Record % of phenotype of offspring Using the information regarding Albinism, determine the Genotypic & Phenotypic Ratios for a couple who are both heterozygous for albinism. (Both parents have normal skin color) Just because the Dominant Trait only needs 1 allele/gene to express itself phenotypically, it DOES NOT mean that it is always the most popular (largest number of individuals with that phenotype) Examples: ◦ Poly-dactylyism ◦ Dwarfism Answer last questions in notebook for WS 1 Dr. Ain Achondroplasia Dwarfism Dominant Trait Begin work using your textbook (pages 8889), notes and/or the ORCA book to assist you with the genetic problems REMEMBER: 1. Determine the parents’ genotypes 2. Use punnett square to list the alleles / genes in the parents gametes 3. Cross the gamete alleles to determine the offspring’s genotypes 4. Record the ratio, fraction or % of the Genotypes 5. Record the ratio, fraction or % of the Phenotypes Note the following Modes of Inheritance 1. Complete dominance; ex: Tongue Rolling (T, t) 2. Incomplete dominance; ex: flower color (R, R’) 3. Sex-Linked Trait; ex: Red-Green Colorblindness (XC, XC, Y) 4. Multiple Alleles; ex: Blood Type (A, B, O) 5. Codominance; ex: Blood Type (ABO) 6. Dihybrid Cross; ex: Pea Pods color and shape (R, r, and Y, y) RR Red RR’ Pink R’R’ white White Board Problem: Cross a White with a Pink flower. Determine Genotypic & Phenotypic Ratio of offspring. White flower genotype R’R’ Pink Flower genotype R R’ Determine the gamete combination & place in Punnett square R R’ R’ RR’ R’R’ R’ RR’ R’R Genotypic and Phenotypic Ratios ½ RR’ ½ R’R’ ½ Pink ½ white White Board new problem: Cross a red flower with a white flower Genes located on Sex Chromosomes (X &/or Y) Which sex (in humans) will express a phenotype of a trait with only 1 gene/allele for the trait? How will a punnett square be arranged? Example: Red/Green Colorblindness White Board Problem: Cross a Heterozygous female for normal color vision with a normal male for color vision. What are the GR and PR for their offspring. Parents Genotype ◦ Mom - XC Xc x ◦ Dad - XC y Place in Punnett square XC Xc X C XCXC XCXc y XC y Xc y Genotypic Ratio ◦ ◦ ◦ ◦ ¼ ¼ ¼ ¼ XCXC XCXc XC y Xc y Phenotypic Ratio ½ Female normal color vision ¼ Male normal color vision ¼ male colorblind White board problem: Cross a colorblind dad with Homozygous mom for normal color vision. Determine the GR and PR of the offspring. 3 Alleles for ABO Blood Type Trait A B O - Alleles (genes) [Multiple Alleles] Variants (phenotypes) Possible are: Blood Type AB [Codominance] Blood Type A Blood Type B Blood Type 0 Phenotype AB A B O Genotype(s) AB AA or AO BB or BO OO White Board Problem: Cross AB blood type With 0 blood type. Give the GR & PR. AA AO BB BO AB OO Cross with 2 traits! Remember that both letters for the possible allele combinations within a gamete, need to be determined Example: A homozygous round and heterozygous yellow pea is crossed with a wrinkled and green pea. Determine the gamete combinations using Rr and Yy letters. Parent Genotype: ◦ RRYy ◦ rryy Parents gamete combinations are: RY and Ry crossed with ry ry RY Ry RrYy Rryy Cross a Heterozygous Round and Heterozygous Yellow pea with a wrinkled and green pea. Show your work completely White board – ABO problem… ◦ Cross a Heterozygous B with a O person ◦ Determine the genotypic and phenotypic ratio of their offspring White board – Sex-linked problem… ◦ Cross a Colorblind man with a Homozygous normal color vision woman. ◦ Determine the genotypic and phenotypic ratio of their offspring Note the 2 traits Below – R & Y green POD color yellow Constricted POD Shape smooth Cross a Heterozygous Tongue roller with no widow’s peak with a non-tongue roller that is Heterozygous for a widow’s peak. [You may use T & t for tongue rolling and W & w for widow’s peak; Tongue rolling and widow’s peak are both dominant alleles.] Determine the Genotypic and Phenotypic ratio for the above cross. 1. Determine the genotype of the parents 2. Determine the gamete combinations with 2 traits 3. Place the gamete combinations into a punnett square (2 different letters per gamete) 4. Using the punnett square, figure each offspring combination (4 letters) 5. Determine the genotypic ratio (%, fraction or ratio 6. Determine the phenotypic ratio (%, fraction or ratio Ttww X ttWw Tw; tw X tW; tw Tw tw tW TtWw ttWw tw Ttww ttww Final Genotypic ratio: Final Phenotypic ratio: Cross a Heterozygous Tongue roller; Heterozyous Widow’s Peak with a person who has the same Genotype for these 2 traits. Determine the Genotypic and Phenotypic ratio of this cross. A heterozygous Tongue roller with no widow’s peak is crossed with a heterozygous Tongue roller & heterozygous for widow’s peak. Determine the genotypic and phenotypic ratio Answer key available AFTER you have completed the worksheet… More practice problems available… If completely finished… extra practice problems - challenge ws is available.