Download lab #12: cells - Waconia High School

LAB #12: CELLS
THE BASIC UNIT OF LIFE
PURPOSE
1. OBSERVE LIVING & NON-LIVING
CELLS UNDER THE MICROSCOPE
2. STUDY & LOCATE, USING THE
MICROSCOPE, 5 CELL PARTS.
3. COMPARE THE CELL PARTS FOUND IN
PLANT & ANIMAL CELLS.
PART A: CELL MEMBRANE & NUCLEUS
1. Select a prepared “Nervous Tissue” and
a “Muscle Tissue” slide.
2. Observe under LOW, then HIGH Power.
3. Draw several CELLS of each tissue;
LABEL the cell membrane & nucleus.
PART B: CELL MEMBRANE & CYTOPLASM
1.
2.
3.
4.
5.
PLACE A DROP OF METHYLENE
BLUE STAIN ON A SLIDE.
SCRAPE THE INSIDE OF YOUR
CHEEK WITH A TOOTHPICK.
MIX CHEEK CELLS INTO THE
METHYLENE BLUE STAIN.
ADD COVERSLIP & EXAMINE
UNDER LOW, THEN HIGH POWER.
DRAW SEVERAL CELLS; LABEL:
CELL MEMBRANE & CYTOPLASM.
PART C: CELL WALL
1. USE A RAZOR BLADE TO
SLICE OFF A THIN SECTION
OF CORK FROM THE SIDE.
2. PREPARE A SLIDE MOUNT.
3. EXAMINE UNDER LOW
THEN HIGH POWER.
4. DRAW SEVERAL CELLS;
LABEL: THE CELL WALL.
PART D: NUCLEUS
1. SNAP AN ONION SCALE
IN HALF AND PEEL OFF
A THIN LAYER OF TISSUE.
2. PLACE ON SLIDE & ADD A
DROP OF IODINE (I-KI)
STAIN TO ONION.
3. ADD COVERSLIP & VIEW
UNDER LOW & HIGH POWER.
4. DRAW THE CELLS; LABEL:
THE NUCLEUS, CELL MEMBRANE,
AND CELL WALL.
PART E: CHLOROPLASTS
1.
PREPARE WET MOUNT OF
AN ELODEA LEAFLET.
2. VIEW UNDER LOW, THEN
HIGH POWER.
3. LOCATE GREEN, OBLONG
CELLS WITH SMALL GREEN
ORGANELLES INSIDE OF IT.
(THEY MAY BE MOVING!)
4. DRAW THE CELLS; LABEL:
CELL WALL & CHLOROPLASTS
http://video.google.com/videoplay?docid=1015732232858582837&q=ELODEA+CE
LLS&total=7&start=0&num=10&so=0&typ
e=search&plindex=5
PART F: UNKNOWNS
1. VIEW SLIDES OF “UNKNOWNS”
LABELLED: “A,” “B,” & “C”
UNDER LOW, THEN HIGH POWER.
2. DRAW SEVERAL CELLS OF EACH;
LABEL (ONLY IF PRESENT): CELL
MEMBRANE, CELL WALL,NUCLEUS,
CHLOROPLASTS, CYTOPLASM.
3. DESIGNATE IF EACH ONE IS A
ANIMAL OR PLANT CELL
POST-LAB ANALYSIS
1. WHAT ARE 3 STRUCTURAL DIFFERENCES
BETWEEN ANIMAL AND PLANT CELLS?
2. WHAT ARE THE FUNCTIONS OF EACH OF
THE 5 ORGANELLES/STRUCTURES YOU
OBSERVED?