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Introduction to Muscle Tissue
Introduction to Muscle Tissue
Skeletal Muscle
- red fibres
- white fibres
- intermediate fibres
-overall shape
-HAZI structure
-triads
-connective tissue elements
Cardiac Muscle
- cardiomyocytes
- Purkinje fibres
-overall shape
-intercalated disk
-diads
-communication and coordination
Smooth Muscle
-overall design and function
Myoepithelial Cells
Hypertrophy vs. Hyperplasia
Other cells (including myofibroblasts)
Skeletal Muscle
- red fibres
- white fibres
- intermediate fibres
It’s HAZI to me!
Skeletal Muscle
Skeletal Muscle
Skeletal Muscle
Skeletal Muscle
Skeletal Muscle Connective Tissue Layers
Muscle spindle apparatus
endomysium
perimysium
epimysium (not shown)
Skeletal Muscle
Cardiac Muscle – cross section
Cardiac Muscle – longitudinal section
Cardiac Muscle – tangential section
Cardiac Muscle – longitundinal, higher power section
Cardiac Muscle – longitundinal, higher power view
Cardiac Muscle – longitundinal, higher power electron microscopic view
Cardiac Muscle – Purkinje fibres
Cardiac Muscle – Purkinje fibres and A-V node
Smooth Muscle – LM and TEM
Smooth Muscle – various angles of presentation
Smooth Muscle – various angles of presentation
Smooth Muscle – nuclear wiggles
Myoepithelial Cells – rapid glandular release
Lab #2 – Muscle Tissue
Lab #2 – Muscle Tissue
Objectives
-Learn how slides are made in more detail
-Introduction to histological stains
-Skeletal muscle – and white muscle disease
-Cardiac muscle – and sarcocysts
-Smooth Muscle – and myometrial hyperplasia
Lab #2 – Muscle Tissue
how slides are made
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Tissue preservation
Clearing and dehydration
Embedding in paraffin or plastic
Sectioning – expanding on a warm water bath
Mounting on a glass slide
De-waxing
Staining
Coverslipping
How (and why) are histology slides made?
How are histology slides made?
How are histology slides made?
How are histology slides made?
fixation
processing
slicing and mounting
de-waxing, staining
hematoxylin & eosin
osmium
Masson’s Trichrome
cover slips
How are histology slides made?
fixation
processing
slicing and mounting
de-waxing, staining
hematoxylin & eosin
osmium
Masson’s Trichrome
cover slips
How are histology slides made?
fixation
processing
slicing and mounting
de-waxing, staining
hematoxylin & eosin
osmium
Masson’s Trichrome
cover slips
How are histology slides made?
fixation
processing
slicing and mounting
de-waxing, staining
hematoxylin & eosin
osmium
Masson’s Trichrome
cover slips
How are histology slides made?
fixation
processing
slicing and mounting
de-waxing, staining
hematoxylin & eosin
osmium
Masson’s Trichrome
cover slips
How are histology slides made?
fixation
processing
slicing and mounting
de-waxing, staining
hematoxylin & eosin
osmium
Masson’s Trichrome
cover slips
How are histology slides made?
fixation
processing
slicing and mounting
de-waxing, staining
hematoxylin & eosin
osmium
Masson’s Trichrome
cover slips
Lab #2 – Muscle Tissue
Fixatives for Histology
- formalin
- other aldehydes
- osmium tetroxide
- air drying
- alcohols
- freeze fixation
Histological Staining
- hematoxylin & eosin: general cellular detail
- Masson’s trichrome: collagen
- Wright’s blood stain: granulocytes
- osmium tetroxide: lipid
- silver nitrate: reticular fibres
Lab #2 – Muscle Tissue
Fixatives for Histology
- formalin
- other aldehydes
- osmium tetroxide
- air drying
- alcohols
- freeze fixation
Histological Staining
- hematoxylin & eosin: general cellular detail
- Masson’s trichrome: collagen
- Wright’s blood stain: granulocytes
- osmium tetroxide: lipid
- silver nitrate: reticular fibres
- periodic acid Schiff: glycogen and other carbohydrates
Lab #2 – Muscle Tissue
Fixatives for Histology
- formalin
- other aldehydes
- osmium tetroxide
- air drying
- alcohols
- freeze fixation
Histological Staining
- hematoxylin & eosin: general cellular detail
- Masson’s trichrome: collagen
- Wright’s blood stain: granulocytes
- osmium tetroxide: lipid
- silver nitrate: reticular fibres