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Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
I. Personal
Office Address
Citizenship:
Date of Birth
Aab Cardiovascular Research Institute
University of Rochester School of Medicine & Dentistry
601 Elmwood Avenue, Box CVRI
Rochester, New York 14642
P.O. Box 76
Spencerport, NY 14559
Office: (585)-276-9789
Fax: (585) 276-9830
E-mail: [email protected]
United States
June 30, 1962 in Syracuse, NY
Family Status
Two children (Alexander, 15; Erika, 11)
Mailing Address
Contact Numbers
II. Education/Training
Years
Degree
Field of Study
Institution
Mentor(s)
1981-86
B.S.
1986-88
M.S.
1988-92
Ph.D.
Post-Doc
State University of New
York at Cortland
New York Medical
College
New York Medical
College
University of Texas M.D.
Anderson Cancer Center
David F. Berger, Ph.D.
Elliott B. Mason, Ph.D.
Alden V. Loud, Ph.D.
Piero Anversa, M.D.
Michael B. Stemerman, M.D.
1992-95
Biology/Physical
Education
Experimental
Pathology
Experimental
Pathology
Molecular/Dev
Biology
Eric N. Olson, Ph.D.
III. Academic Appointments
2003-Present
1999-2003
1995-1999
Associate Professor of Medicine and Associate Director of the Aab Cardiovascular
Research Institute of the University of Rochester School of Medicine and
Dentistry, Rochester, New York
Assistant Professor of Medicine and Pathology and Laboratory Medicine in the
Center for Cardiovascular Research, University of Rochester School of Medicine
and Dentistry, Rochester, New York
Assistant Professor in the Department of Physiology, Medical College of
Wisconsin, Milwaukee, Wisconsin
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 2
IV. Professional Consultantships
1998-2001
2001-Present
Introgen Therapeutics, Inc., Houston, Texas
Socratech Inc., Rochester, New York
V. Honors and Awards
1992
1993
1997
2001
2003
2007
Helen S. Page Memorial Ph.D. Award
Post-Doctoral National Research Service Award, National Institutes of Health,
MD Anderson Cancer Center, Smooth Muscle Cell Differentiation
Manitowoc Heart-A-Rama Research for Life Award-AHA, Wisconsin Affiliate
Elected as Fellow of the American Heart Association
Who’s Who in Medicine and Healthcare
Inducted into the Gates-Chili High School Hall of Fame
VI. Professional Organizations
1988-present
1994-present
2000-2008
2001-present
2004-2007
American Association for the Advancement of Science
North American Vascular Biology Organization
Society for Developmental Biology
Fellow of the American Heart Association
American Society for Biochemistry and Molecular Biology
VII. University Committee Assignments
1996-1999
1998-1999
1999-2003
2000
2000-2003
2001-Present
2001-2003
2001
2001
2001
2003-Present
2004-Present
2004
2005
2005
2006
2006
2007
2007-Present
2007-Present
2007-2008
2008
2008-Present
Research Affairs Committee , Medical College of Wisconsin
Chair, Research Affairs Committee, Medical College of Wisconsin
Human Use of Radioisotopes Committee , University of Rochester
Transgenic Facility Advisory Committee, University of Rochester
University Committee on Animal Resources, University of Rochester
Reviewer, Strong Children’s Research Center Research Dev. Award
Member, Radiation Safety Advisory Board, University of Rochester
Member, UCAR Forms Subcommittee
Member, UCAR Breeding Subcommittee
Member, Steering Committee for GEBS Cluster Realignment
Member, Applications Committee for PHD GEBS Cluster
Member, Steering Committee for Pathways of Human Disease Cluster
Chair, CVRI Faculty Search Committee
Member, Intellectual Property Advisory Committee
Member, Vivarium Planning Committee for new CVRI
Ad Hoc Committee Member for Promotions
Member, Equipment Planning Committee for new CVRI
Member, External Review of Center for Aging & Developmental Biology
Functional Genomics Center, Advisory Group
Member, Transgenic Mouse Core Advisory Committee
Member, Search Committee, Cardiology Chief
Member, Search Committee, Senior Assoc Dean for Graduate Education
Member, URMC Core Oversight Committee
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 3
VIII. Local/National/International Assignments
1998-99
1999-2002
1999-2001
1999
2001-2004
2001-2004
2002-Present
2002-2007
2003-Present
2004-2005
2004
2005
2005
2006-Present
2007
2008
2008
2007-2009
2009
2009
2009
Study Section Member, American Heart Association, Affiliate, Great
America 2B
Study Section Member, American Heart Association, Affiliate, NE2
Study Section Member, American Heart Association, National, Vascular
Wall Biol I
Co-Chair, Signaling and Vasc Biol, NAVBO, Washington, D.C.
Chair, Meritorious Awards Committee, NAVBO
Member, New York State AHA Affiliate Research Committee
Reviewer, Science Foundation Ireland
Reviewer, Philip Morris External Research Program
Reviewer, John Sealy Memorial Endowment Fund for Biomedical Res.
Member, Program Committee for 2005 NAVBO Meeting, Chicago, IL
Chair, SMC Proliferation and Migration Session, AHA, New Orleans
Chair, Vasculomics Session, NAVBO Annual Meeting, Chicago
Ad hoc reviewer for NIH Special Emphasis Panel and R43 grants
Member, External Advisory Committee for COBRE @ UN, Reno
Co-Chair, NEA 5 (Gene Exp: Cardiovascular Development) AHA Study
Group
Co-Chair, ATVB Plenary Session on “Stem Cells”, Atlanta, GE
Member, NIH SEP (ZRG1 CVS-E) CV Dev and Stem Cells
Member, ATVB Program Committee
Co-Chair, ATVB Session on Vascular Cell Dev and Differentiation
AHA Science Classification Task Force
Chair, Session on SMC-Specific Knockouts, FASEB Summer Research
Conference on SMC, Lucca, Italy (August 2-7)
IX. Community Service
1996-1998
1999-2001
2000-Present
2008-Present
Center for Science Excellence “Mini Medical School” Med College Wisconsin
Workshop Mentor for Victor Central School District
Visiting Science Teacher, Our Mother of Sorrows Catholic School
Community Outreach for the Aab CVRI
X. Ad Hoc Reviewer
American Journal of Pathology
American Journal of Physiology, Cell Physiology
Anatomical Record
Biotechniques
Cardiovascular Research
Circulation
Developmental Biology
Genome Biology
Human Molecular Genetics
Hypertension
Journal of Cell Biology
Journal of Cellular Physiology
Journal of Clinical Investigation
Journal of Molecular Biology
Molecular Cellular Biology
Molecular Genetics & Genomics
Physiological Reviews
PNAS
Stem Cells
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 4
XI. Editorial Boards
1998-2001
2000-2007
2003-2007
2000-2007
1997-present
2004-present
2004-present
2007-present
Journal of Vascular Research
American Journal of Physiology, Heart & Circulatory Physiology
Current Drug Targets - Cardiovascular and Haematological Disorders
Journal of Molecular & Cellular Cardiology
Circulation Research
Journal of Biological Chemistry
Physiological Genomics
Associate Editor, Arteriosclerosis, Thrombosis & Vascular Biology
动脉硬化 血栓与血管生物学
XII. Teaching Experience
1997-1999
1998
1998
2000-2006
2001-2003
2001-2003
2005
2000-Present
2002-Present
2007-Present
2008-Present
Course Director, Problems in Molecular Physiology, Medical College of
Wisconsin
Pathology for Basic Scientists , one lecture on Vascular Pathobiology,
Medical College of Wisconsin
M4-Elective, Medical Problem Solving: Understanding Disease
Cardiovascular Basic Science Discussant, Medical College of Wisconsin
Coordinator, Signaling And Gene Expression (SAGE) Seminars
PHP440/BME 484 Topics in Vascular Biology, Univ. Rochester Med Ctr
Adult Inpatient Clerkship—Bed and Bench, Univ. of Rochester Med Ctr
MD/PhD Program Scientific Reasoning in Medicine
PTH593 Smooth Muscle Pathobiology, University of Rochester Medical
Center (3 hrs)
PTH509/510 Pathways of Human Disease, Univ. of Rochester Medical
Center (3 hrs lecture, 1 hr lab, 1 hr paper discussion)
Course Co-Director (2007 only) and lecturer (4 hrs), CVS-401,
Cardiovascular Biology and Disease
GEN508 Genomics and Systems Biology (2.5 hrs)
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 5
XIII. Graduate Students (students obtaining PhD in Miano lab are in red)
Tammy Nuckolls
Heather Stefaniak
Shawn Jobe
James Bruce
Christina Stafford
Marcelo Nobrega
Jeffrey Streb
Xinchun Pi
Owen Felder
Owen Altland
Ruolan Han
Lan Tang (Linda)
Matthew Grunert
Hai-Ou Duan
Ann Rossi
Joseph Senn
Tiefei Dong
Lungkuo Tai
Fred Strathmann
David Lomb
Ying Yang
Lea Gunnell
Ting-Hein Lee
Ryan Hoefer
David Nagel
Alayna Loiselle
Vincent Fong
Jody Davis
Jing Ni
Matthew Schoell
Kai Sun
Vincent Fong
Pauline Ocaya
Julie Smith
Robert Bell
Vivek Nanda
Erik Larsson
Xun Tang
Medical College of Wisconsin, SPUR, 1997
Medical College of Wisconsin, SPUR, 1998
Medical College of Wisconsin, Dissertation Committee, 1995-97
Medical College of Wisconsin, Dissertation Committee, 1998-1999
Medical College of Wisconsin, Dissertation Committee, 1998-1999
Medical College of Wisconsin, Dissertation Committee, 1999-2000
URMC, Dissertation Thesis Advisor, 2000-2004, PostDoc @ UCLA
URMC, Dissertation Committee, 2000-2004
URMC, Rotation Student, 2000
URMC, Rotation Student, 2000
URMC, Rotation Student, 2001
URMC, Rotation Student, 2002
URMC, Independent Research on Zebrafish Calponins, 2002
URMC, Dissertation Committee, 2002-Present
URMC, Rotation Student 2002
URMC, Dissertation Committee, 2002-2003
URMC, Dissertation Committee, 2003-2006
URMC, Dissertation Committee, 2003-2006
URMC, Dissertation Committee, 2003-2008
URMC, Dissertation Committee, 2003-2008
URMC, Dissertation Committee, 2003-2009
URMC, Dissertation Committee, 2004-Present
URMC, Dissertation Thesis Advisor, 2005-2008
URMC, Chair Dissertation Defense Committee, 2005
URMC, Dissertation Committee, 2006-2008
URMC, Dissertation Committee, 2006-2009
URMC, Rotation Student 2006
URMC, Summer Student 2006
URMC, Chair Dissertation Defense Committee, 2006
URMC, Chair Dissertation Defense Committee, 2007
Medical College of Wisconsin, External Committee Member, 2007
URMC, Qualifying Exam Committee 2007
University of Orebro, Sweden 2006
URMC, Rotation Student 2008
URMC, Dissertation Thesis Advisor, 2009-Present
URMC, Dissertation Thesis Advisor, 2009-Present
University of Gothenburg, SWEDEN, Opponent on April 17, 2009
URMC, Summer Student, 2009
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 6
XIV. Post-doctoral Fellows
Jiyuan Chen, MD, PhD
10/16/99 – 3/15/03
Hesheng Ou, MD, PhD
Vineet Dua, MD
Qiang Sun, PhD
Yumei Yang, PhD
Xiaochun Long, PhD
Masaaki Imamura
11/1/99 – 6/15/01
Sable Awardee, 2000-2001
11/1/03-10/16/06
4/12/03-11/15/2004
1/17/05-Present
4/1/08 – Present
Presently, Associate Professor, Division of
Cardiology, Zhongda Hospital, China
Presently, Instructor at Baylor Coll of Med
Cardiologist, Strong Memorial Hospital
Presently, Post-Doc in Frankfurt, Germany
Post-Doc at Emory University
Research Assistant Professor in Miano Lab
Post-Doc in Miano Lab
XV. Major Research Interests
1.
2.
3.
4.
5.
Transcriptional control of smooth muscle cell differentiation
Smooth muscle cell developmental biology
Comparative genomics and CArG element discovery
Role of SRF and Myocardin in Alzheimer’s angiopathy
Role of Myocardin as master regulator in SMC contractile phenotype
XVI. Invited Seminars
1990-1996
1. “Early Induction of c-myc Following Balloon Injury in the Rat Aorta.” Federation Of
American Societies Of Experimental Biology (FASEB), Washington, D.C. April 20, 1990.
2. “Developmental Expression and Promoter Analysis of a SMC-Restricted Gene.” American
Heart Association Annual Meeting, Dallas, TX, November 12, 1994.
3. “Smooth Muscle Cell Marker Expression During Development” Federation Of American
Societies Of Experimental Biology (FASEB), Atlanta, GA. April 12, 1995.
4. “Smooth Muscle Cell Growth & Differentiation." Sponsored by Parke-Davis, Bruce
Markham, Ph.D, (Host). September 18, 1995.
5. "Molecular Control of Smooth Muscle Differentiation." Gordon Research Conference,
February 20, 1996.
1997
1. “Hierarchical Specificity of Smooth Muscle Marker Expression.” University of Wisconsin,
Gary Lyons (Host). May 13, 1997.
2. “Retinoids and Vascular Smooth Muscle Cell Biology.” Fourth Franz-Volhard Symposium,
Berlin, Germany, June 27, 1997
3. “Strategies for Studying Smooth Muscle Cell-Specific Gene Expression.” Medical College of Wisconsin,
Department of Anatomy and Cell Biology. Maya Sieber-Blum, Ph.D. ( Host). December 17, 1997.
4. “Retinoid Therapy: A New Pharmacological Approach to Limit Restenosis Following Angioplasty?”
University of Texas, M.D. Anderson Cancer Center, Houston, Texas. Scott Lippmann (Host). December 30,
1997.
1998
1. “Molecular Control of Smooth Muscle Calponin Expression.” International Workshop on
Airway and Vascular Smooth Muscle Function in Health and Disease. Sundance, Utah,
October 30, 1998.
2. “Coronary Restenosis: A Translational Approach.” Cardiology Grand Rounds, Medical
College of Wisconsin. David Gutterman, M.D. (Host). November 19, 1998.
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 7
XVI. Invited Seminars (continued)
3. “Novel Approaches for the Treatment of Vascular Occlusive Diseases.” Introgen
Therapeutics, Inc., Houston, TX . Sunil Chada, PhD, (Host). December 10, 1998.
1999
1. “Novel Strategies for Adenoviral-Mediated Gene Transfer to Vascular Smooth Muscle
Cells.” Dysphagia Research Group, Medical College Of Wisconsin. Reza Shaker,
M.D.,(Host). January 5, 1999.
2. “New Approaches to Studying Vascular Smooth Muscle Cell Growth and Differentiation Control.” Center for
Cardiovascular Research, University of Rochester, Rochester, NY.
Bradford Berk, MD, Ph.D., (Host). February 12, 1999.
3. “Transcriptional Control of Smooth Muscle Calponin.” University of Calgary, Calgary,
Canada. Michael P. Walsh, Ph.D., (Host). March 5, 1999.
4. “New Approaches for the Prevention of Vascular Restenosis: Retinoids and SMC-Specific
Gene Targeting.” The New York Hospital Cornell Medical Center. Krishna Kandarpa
M.D. Ph.D., (Host). May 12, 1999.
5. “Retinoic Acid: A New Pharmacological Intervention for Vascular Occlusive Diseases?”
University of Texas Health Science Center at San Antonio. Tony Firulli, Ph.D., (Host).
May17, 1999.
6. “BAC on Track with the Transcriptional Regulation of Smooth Muscle Calponin.” Medical
College of Wisconsin. William Chilian, PhD, (Host). June 24, 1999.
7. “Experimental Therapies for Treating Cardiovascular Diseases - Contemporary Issues”
University of Rochester Medical Center, Jay H. Stein, M.D., moderator. December 17,
1999.
2000
1. “Transcriptional Regulation of Smooth Muscle Calponin.” FASEB Summer Research
Conferences: Smooth Muscle. Snowmass, Colorado, July 22, 2000.
2. “Using BACs to Delineate Transcriptional Regulatory Elements of the Smooth Muscle
Calponin Gene.” Columbia University. Peter Cserjesi, Ph.D., (Host). October 13, 2000.
3. “Models for Studying the Transcriptional Control of Smooth Muscle Calponin” Boston
Biomedical Research Institute. Albert Wang, PhD, (Host). November 1, 2000.
2001
1. “Retinoid-Mediated Effects on Smooth Muscle Cell Growth and the Vessel Wall’s Response
to Injury” University of Rochester Medical Center, Dept. Pathology, Oncology, and
Molecular Medicine. Steve Spitalnik (Host). February 5, 2001.
2. “BACing for Regulatory Elements Controlling Smooth Muscle Cell Differentiation”
University of Rochester Medical Center, Center for Aging and Developmental Biology.
David Pierce (Host). September 18, 2001.
3. “Retinoids and Vascular Smooth Muscle Cell Biology” New York Medical College, Dept.
Pathology. Caroline Smith (Host). October 3, 2001.
2002
1. “Retinoid Response Genes in Vascular Smooth Muscle Cells” Heidelberg – Berlin First Symposium on
Retinoids in Vascular and Renal Disease – new therapeutic indications? German Cancer Research Center.
Heidelberg, Germany. Jeurgen Wagner and Christos Zouboulis (Hosts). March 16, 2002.
2. “Retinoid Response Genes in Vascular Smooth Muscle” NAVBO Workshop on Nuclear Receptors, Salt Lake
City, UT, April 5, 2002.
3. “Vascular Smooth Muscle Cell Differentiation and Growth Control: Use of Genomics and Pharmaceutics”
University of Rochester Medical Center, Division of Neonatology, Michael O’Reilly (Host). June 11, 2002.
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 8
XVI. Invited Seminars (continued)
4. “Sifting a BAC for Regulatory Elements Controlling Smooth Muscle Calponin Expression” New York
Medical College, Dept. Pharmacology. Nader Abraham (Host). August 9, 2002.
5. “Genomico-Bioinformatic Strategies for Studying Vascular Smooth Muscle Cell Differentiation” University
of Rochester Medical Center. Socratech symposium on SMC. Berislav Zlokovic (Host). October 1, 2002.
6. “Characterization of a Unique Pulmonary Artery SMC Line” University of Rochester Medical Center.
Pulmonary Division Seminar. Patricia Sime (Host). October 8, 2002.
7. “BACing for Regulatory Elements Controlling Smooth Muscle Cell Differentiation” Morehouse School of
Medicine, Cardiovascular Research Institute. Gary Gibbons (Host). October 15, 2002.
8. “Mining a BAC for Regulatory Elements Controlling Smooth Muscle Calponin Expression” Maine Medial
Center, Center for Molecular Medicine. Tom Maciag (Host). October 24, 2002.
9. “Retinoids” American Heart Association, November 17, 2002
10. “Genomico-Bioinformatic Approach to Studying Smooth Muscle Cell Differentiation” University of Chicago,
Department of Medicine. Julian Solway (Host). November 18, 2002.
2003
1. “Retinoid Biology in Smooth Muscle Cells” University of Indiana, Center for
Cardiovascular Research. Keith March (Host). February 4, 2003.
2. “Genomico-Bioinformatic Approach to Studying Smooth Muscle Cell Differentiation”
University of Indiana, Department of Physiology. Paul Herring (Host). February 5, 2003.
3. “Genomico-Bioinformatic Approach to Studying Smooth Muscle Cell Differentiation”
University of North Carolina, Chapel Hill, Carolina Cardiovascular Biology Center. Cam Patterson (Host).
April 10, 2003.
4. “Genomico- Bioinformatic Approach to Studying Smooth Muscle Cell Differentiation”
4th Annual ATVB Meeting, Washington, DC. May 9, 2003.
5. “Genomico-Bioinformatics and SMC Differentiation” FASEB Summer Conference on
Smooth Muscle Cells. Snowmass, CO. July 6, 2003.
6. “Genomico-Bioinformatics and SMC Differentiation” XIIIth International Symposium on Atherosclerosis.
Kyoto, Japan. October 1, 2003.
7. “Retinoid Effects in the Vessel Wall and the Smooth Muscle Cell Transcriptome” Role of Nuclear Receptors
in Cardiovascular Disease (Hot Topics in Endocrinology). Coronado, CA. October 10, 2003.
8. “Molecular Control of Smooth Muscle Differentiation” Vascular Biology Center in the Medical College of
Georgia. David Stepp/Brenda Lilly (Hosts). November 4, 2003.
9. “Transcriptional Regulation of Smooth Muscle Cell Differentiation” Department of Biology, University of
Rochester. Cheeptip Benyajati (Host). November 10, 2003.
10. “Genomico-Bioinformatics and SMC Differentiation” Department of Physiology, University of Tennessee.
Gadiparthi Rao (Host). November 20, 2003.
2004
1. “What’s New with the Control of Smooth Muscle Cell Differentiation?” Center for Cardiovascular
Sciences, Albany Medical College. John Schwarz, (Host). May 5, 2004.
2. “Genomics of Smooth Muscle Calponin Expression” Department of Pharmacology & Physiology, Drexel
University. Robert Moreland (Host). May 24, 2004.
3. “Transcriptional control of vascular smooth muscle differentiation” Department of Pathology, University
of Rochester School of Medicine. Robert Mooney (Host). June 14, 2004.
4. “Molecular Control of Smooth Muscle Cell Differentiation” Distinguished Scientist Seminar Series,
University of South Alabama College of Medicine. Thomas Lincoln (Host). October 21, 2004.
5. “Role of SRF-Myocardin in Muscle Cell Differentiation” Department of Biochemistry, Medical College of
Wisconsin. Ravi Misra (Host). October 27, 2004.
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 9
XVI. Invited Seminars (continued)
2005
1. “Investigating Vascular Smooth Muscle Cell Pathobiology in the 21st Century” Biology Department,
SUNY Cortland. Larry Klotz, (Host). April 6, 2005.
2. “Serum Response Factor and the Mammalian CArGome” Department of Pathology, Yale University, Wang
Min (Host). June 7, 2005.
3. “Serum Response Factor and the Mammalian CArGome” Department of Genetics, University of
Pennsylvania, Christian Stoeckert (Host). June 20, 2005.
4. “SRF and Mammalian CArGome” Department of Physiology, Texas A&M University, Emily Wilson
(Host). September 8, 2005.
5. “SRF and the CArGome” Muscling Through the Ages Symposium at University of Texas Southwestern
Medical School, Eric Olson (Host). September 24, 2005.
6. “SRF and the CArGome” University of Rochester, CVRI. December 7, 2005.
2006
1. “SRF and the CArGome” Department of Pharmacology and Physiology, University of Nevada, Reno, Dean
Burkin, PhD (Host). March 9, 2006.
2. “SRF and the CArGome” Department of Molecular and Cellular Biology, Roswell Park Cancer Institute,
Kenneth Gross, PhD (Host). April 20, 2006.
3. “SRF and the CArGome” University of Virginia, Charlottesville, Gary Owens, PhD (Host). May 18,
2006.
4. “Role of Serum Response Factor and the CArGome in Smooth Muscle Cell Differentiation” FASEB
Summer Conference on Smooth Muscle Cells. Snowmass, CO. July 30, 2006.
5. “Biology of a Retinoid-Induced A-Kinase Anchoring Protein” University of South Alabama College of
Medicine. Thomas Lincoln (Host). November 15, 2006.
6. “Vascular Smooth Muscle Cell Differentiation in Danio rerio” University of Örebro (Sweden). Leif
Eriksson (Host). December 7, 2006.
7. “Retinoid Biology in Smooth Muscle Cells and the Vessel Wall” University of Örebro (Sweden). Allan
Sirsjö (Host). December 8, 2006.
2007
1. “SRF and Myocardin in Smooth Muscle Differentiation and Alzheimer’s Angiopathy” Wayne State
University. Li Li (Host). May 25, 2007.
2. “Bridging Bioinformatics with Experimental Biology” URMC, Dept Biostatistics and Computational
Biology. Jim Cavenaugh (Host). September 21, 2007.
3. “Diverse Roles for SRF-Myocardin in Muscle Biology” Dartmouth Medical School. Kathy Martin (Host).
October 3, 2007.
4. “SRF-Myocardin in Development and Disease” Dept. Pathology, University of Rochester School of
Medicine and Dentistry. Dan Ryan (Host). November 12, 2007.
2008
1. “Diverse Roles for SRF-Myocardin in Muscle Biology” Department of Biochemistry, Vermont University
Medical School. Robert Kelm (Host). March 28, 2008.
2. “Diverse Roles for SRF-Myocardin in Muscle Biology” Aab Cardiovascular Research Institute, University
of Rochester School of Medicine & Dentistry. Jane Sottile (Host). April 30, 2008.
3. “Diverse Roles for SRF-Myocardin in Muscle Biology” Committee on Cell Physiology, University of
Chicago. Nickolai Dulin (Host). May 2, 2008.
4. “Diverse Roles for SRF-Myocardin in Muscle Biology” Division of Cardiology, Emory University School
of Medicine. Kathy Griendling (Host). September 8, 2008.
5. “Diverse Roles for SRF-Myocardin in Muscle Biology” Herman B. Wells Center for Pediatric Research,
Indian University. Anthony Firulli (Host). October 9, 2008.
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 10
XVI. Invited Seminars (continued)
6. “Diverse Roles for SRF-Myocardin in Muscle Biology” Institut für umweltmedizinische Forschung of the
University of Düsseldorf, Germany. Judith Haendeler (Host). November 19, 2008.
2009
1. “Diverse Roles for SRF-Myocardin in Muscle Biology” Department of Pathology, University of Alabama
at Birmingham. Yabing Chen (Host). January 15, 2009.
2. “Diverse Roles for SRF-Myocardin in Muscle Biology” Department of Physiology & Pharmacology,
University of Georgia, Athens. Shiyou Chen (Host). March 30, 2009.
3. “SRF-Myocardin: From Genome to Disease” Department of Medicine, Research in Medicine Seminar.
University of Rochester School of Medicine & Dentistry. David Bushinsky (Host). April 8, 2009.
4. “Diverse Roles for SRF-Myocardin in Muscle Biology” The Sahlgrenska Academy of the University of
Gothenburg, Sweden. Per Lindahl (Host). April 16, 2009.
5. “Retinoid Biology in Vascular Smooth Muscle Cells” Department of Medicine, Columbia University.
William Blaner (Host). June 3, 2009.
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 11
XVII. News/TV Features
1. Rochester Democrat and Chronicle, “Teachers Learn How to Inspire Future Scientists.” June 1, 2000.
2. Rochester Democrat and Chronicle, “UR Researchers Cheer new Chromosome Map.” July 2, 2000.
3. WOKR, Channel 13 Health News, “Zebrafish Used to Study Cardiovascular Research.” February 19,
2004.
4. Rochester Democrat and Chronicle, “UR Sleuths on DNA Mission.” April 15, 2006.
5. Rochester Democrat and Chronicle, “UR Heart Researchers to Get Own Building.” August 22, 2007.
XVIII. Grant Support
Previous Research Support
Principal Investigator, “Identification of Smooth Muscle Cell-Specific Transcription Factors.” Medical College of
Wisconsin New Faculty Grant. $7,500, 11/15/95-11/14/96.
Principal Investigator, :“Retinoid-Mediated Smooth Muscle Cell Growth Control. American Heart Association
Grant-In-Aid, Wisconsin Affiliate. Total Direct Costs, $60,000; 7/96-6/98.
Principal Investigator,“A Cell-Specific Promoter-Based Approach to Targeting Transgene Expression in Normal
and Diseased Blood Vessels.” The Charles Culpeper Foundation. Total Direct Costs, $22,438; 1/1/98-12/31/98.
Principal Investigator, (10% effort). “Retinoid-Mediated Smooth Muscle Cell Growth and Differentiation
Control.” AHA Scientist Development Grant. Total Direct Costs, $260,000; 1/1/98-12/31/01.
Principal Investigator, (10% effort). “Role of Tissue Transglutaminase in Retinoid-Mediated
Smooth Muscle Cell Responses.” AHA Grant-In-Aid (Affiliate). Total Direct Costs, $180,000; 7/1/01-12/31/02
(Grant relinquished 12/02 to accept funding for AHA Established Investigator Award on 1/1/03.)
Applicant Sponsor, (0% effort). “Local Delivery of Retinoid-Related Compounds to the Injured Vessel Wall.”
AHA Postdoctoral Fellowship for Jiyuan Chen. Total Direct Costs, $103,605; 7/1/00-6/30/03
Applicant Sponsor, (0% effort). “Transcriptional Regulation of Myocardin Gene Expression.” AHA Postdoctoral
Fellowship for Xiaochun Long. Total Direct Costs, $76,000; 7/1/06 – 06/30/08.
Active Research Support
Principal Investigator, (20% effort). “Molecular Control of Smooth Muscle Calponin
Expression.” NIH RO-1 NHLBI 10-HL62572-10A1. Total Direct Costs, $900,000; 07/01/07 – 06/30/12.
Principal Investigator, (25%). “Bifunctionality of Myocardin in Myogenesis.” NIH RO-1 HL091168-01.
Total Direct Costs, $1,000,000; 07/01/08 – 06/30/12.
Core B Director, (15%). “Vascular Inflammation and Atherosclerosis.” NIH PO-1 NHLBI 01-077789. Total
Direct Costs in Year 1, $103,450; 8/1/05 – 07/31/10.
Principal Investigator, (15%). “SRF/MYOCD: New Targets in Alzheimer’s Neurovasculature.” Phase II STTR,
AG026950-02. Total Direct Costs, $172,664; 06/15/08 – 05/31/10.
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 12
Pending Support
Principal Investigator, (35% effort). “Analysis of a Retinoid-Induced Tumor Suppressor in
SMC.” NIH RO-1 NHLBI 05-HL70077. Total Direct Costs, $1,407,938; 11/01/08 – 10/31/13.
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IX. Publications (* Corresponding Author)
1. Miano, J.M., Holland, J.A., Pritchard, K.A., Jr., Rogers, N.J. and Stemerman, M.B.* Low density
lipoprotein-mediated endothelial cell perturbation: Effects on endothelial cell eicosanoid metabolism. In
(Samuelsson,B. and P.K.Y. Wong, Eds.) Advances in Prostaglandin, Thromboxane and Leukotriene Research.
Raven Press, New York, Vol. 19, pp. 248-254, 1989.
2. Miano, J.M.*, Tota, R.R., Vlasic, N., Danishefsky, K. and Stemerman, M.B. Early proto-oncogene expression
in rat aortic smooth muscle cells following endothelial removal. Am.J.Pathol. 137:761-765, 1990.
3. Miano, J.M. Smooth muscle cell immediate early gene expression following vascular injury: Relationship to
growth factor gene induction and smooth muscle cell growth. Dissertation. University Microfilms Inc., Ann
Arbor, MI 1992.
4. Miano, J.M., Vlasic, N., Tota, R.R. and Stemerman, M.B.* Smooth muscle cell immediate-early gene and
growth factor activation follows vascular injury: A putative in vivo mechanism for autocrine growth.
Arterioscler.Thromb. 13:211-219, 1993.
5. Miano, J.M., Vlasic, N., Tota, R.R. and Stemerman, M.B.* Localization of Fos and Jun proteins in rat aortic
smooth muscle cells after vascular injury. Am.J.Pathol. 142:715-724, 1993.
6. Pritchard, K.A.*, O'Banion, M.K., Miano, J.M., Vlasic, N., Bhatia, U.G., Young, D.A. and Stemerman, M.B.
Acute expression of a novel cyclooxygenase gene in rat vascular smooth muscle cells in vitro and in vivo.
J.Biol.Chem. 269:8504-8509, 1994.
7. Martin, J.F., Miano, J.M., Hustad, C.M., Copeland, N.G., Jenkins, N.A. and Olson, E.N.* A Mef2 gene that
generates a muscle-specific isoform via alternative mRNA splicing. Mol. Cell.Biol. 14:1647-1656, 1994.
8. Miano, J.M., Cserjesi, P., Ligon, K., Periasamy, M. and Olson, E.N.* Smooth muscle myosin heavy chain
marks exclusively the smooth muscle lineage during mouse embryogenesis. Circ.Res. 75:803-812, 1994.
9. Miano, J.M., Firulli, A.B., Olson, E.N., Hara, P., Giachelli, C.M. and Schwartz, S.M.* Restricted expression
of homeobox genes distinguishes fetal from adult human smooth muscle cells. Proc.Natl.Acad.Sci., USA.
93:900-905, 1996.
10. Li, L., Miano, J.M., Cserjesi, P. and Olson, E.N.* SM22, a marker of adult smooth muscle, is expressed in
multiple myogenic lineages during embryogenesis. Circ.Res. 78:188-195, 1996.
11. Firulli, A.B., Miano, J.M., Bi, W., Johnson, D., Casscells, W., Olson, E.N., and Schwarz, J.* Myocyte
enhancer binding factor-2 expression and activity in vascular smooth muscle cells: association with the
activated phenotype. Circ.Res. 78:196-204, 1996.
12. Li, L., Miano, J.M., Mercer, B. and Olson, E.N.* Expression of the SM22 promoter in transgenic mice
provides evidence for distinct transcriptional regulatory programs in vascular and visceral smooth muscle cells.
J. Cell Biol. 132:849-859, 1996.
13. Miano, J.M.* and Olson, E.N. Expression of the smooth muscle cell calponin gene marks the early cardiac and
smooth muscle cell lineages during mouse embryogenesis. J.Biol.Chem. 271:7095-7103, 1996.
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Joseph M. Miano, Ph.D.
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14. Miano, J.M.*, Topouzis, S., Majesky, M.W. and Olson, E.N. Retinoid receptor expression and all-trans
retinoic acid-mediated growth inhibition in vascular smooth muscle cells. Circulation. 93:1886-1895, 1996.
15. Miano, J.M.*, Krahe, R., Garcia E., Elliott, J.M. and Olson, E.N. Expression, genomic structure and high
resolution mapping to 19p13.2 of the human smooth muscle cell calponin gene. Gene. 197:215-224, 1997.
16. Rao, R.S., Miano, J.M., Olson, E.N. and Seidel, C.L.* The A10 cell line: a model for neonatal, neointimal, or
differentiated vascular smooth muscle cells? Cardiovasc.Res. 36:118-126, 1997.
17. Koike, G.*, Miano, J.M., Vanvooren, P., Shiozawa, M., Szpirer, C. and Jacob, H.J. Mapping of the rat SM22
gene to chromosome 8q24. Mamm. Genome. 9:76-77, 1998.
18. Firulli, A.B., Han, D., Kelly-Roloff, L., Koteliansky, V.E., Schwartz, S.M., Olson, E.M., and Miano, J.M.* A
comparative molecular analysis of four rat smooth muscle cell lines. In Vitro Cell.Dev.Biol.Anim. 34:217-226,
1998.
19. Zilverman, A., Dave, V., Miano, J.M., Olson, E.N., and Periasamy, M.* An evolutionary conserved promoter
region containing CArG-like elements is crucial for smooth muscle myosin heavy chain gene expression.
Circ.Res. 82:566-575, 1998.
20. Miano, J.M.*, Kelly, L.A., Artacho, C.A., Nuckolls, T.A., Piantedosi, R. and Blaner, W.S. All-trans retinoic
acid reduces neointimal formation and promotes favorable geometric remodeling of the rat carotid artery
following balloon withdrawal injury. Circulation. 98:1219-1227, 1998.
21. Clifford, J.L., Miano, J.M., and Lippman, S.M.* Retinoids and interferons as antiangiogenic cancer drugs. In:
Antiangiogenic Agents in Cancer. Teicher, B.A., Ed., Humana Press, 1999. 355-370.
22. Miano, J.M.*, Garcia, E. and Krahe, R. Radiation hybrid (RH) mapping of human smooth muscle-restricted
genes. In: Molecular Biology of Vascular Disease. Baker, A.H., Ed., Humana Press, 1999. 25-35.
23. Kowal, R.C., Richardson, J.A., Miano, J.M., and Olson, E.N.* EVEC: A novel epidermal growth factor-like
repeat-containing protein expressed by embryonic and diseased adult vasculature. Circ.Res. 84:1166-1176,
1999.
24. Miano, J.M. and Berk, B.C.* NAB2: A transcriptional brake for activated gene expression in the vessel wall?
Am.J.Pathol. 155:1009-1012, 1999.
25. Nobrega, M.A., Shiozawa, M., Koike, G., Jacob, H.J., and Miano, J.M.* Gene structure, promoter analysis,
and chromosomal mapping of the rat smooth muscle calponin gene. Mamm. Genome. 11:115-119, 2000.
26. Molkentin, J.D.*, Antos, C., Mercer, B., Taigen,T., Miano, J.M., and Olson, E.N. Direct activation of a
GATA6 cardiac enhancer by Nkx2.5: Evidence for a reinforcing regulatory network of Nkx2.5 and GATA
transcription factors in the developing heart. Dev.Biol. 217:301-309, 2000.
27. Miano, J.M.*, Carlson, M.A., Spencer, J.J., and Misra, R.P. Serum response factor-dependent regulation of
the smooth muscle calponin gene. J.Biol.Chem., 275:9814-9822, 2000.
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Joseph M. Miano, Ph.D.
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28. Camoretti-Mercado, B., Liu, H.W., Halayko, A.J., Forsythe, S.M., Kyle, J.W., Li, B., Fu, Y., McConville,J.,
Kogut, P., Vieira, J.E., Patel, N.M., Hershenson, M.B., Fuchs, E., Sinha,, S., Miano, J.M., Parmacek, M.S.,
Burkhardt, J.K., and Solway, J.* Physiologic control of smooth muscle-specific gene expression through
regulated nuclear translocation of serum response factor. J.Biol.Chem., 275:30387-30393, 2000.
29. Miano, J.M.* and Berk, B.C. Retinoids: Versatile biological response modifiers of vascular smooth muscle
phenotype. Circ.Res., 87:355-362, 2000.
30. Ou, H., Haendeler, J., Aebly, M.R., Kelly, L.A., Cholewa, B.C., Koike, G., Kwitek-Black, A., Jacob, H.J.,
Berk, B.C., and Miano, J.M.* Retinoic acid-induced tissue transglutaminase and apoptosis in vascular smooth
muscle cells. Circ.Res., 87:881-887, 2000.
31. Chen, J., Maltby, K.M., and Miano, J.M.* A novel retinoid-response gene set in vascular smooth muscle cells.
Biochem.Biophys.Res.Comm. 281:475-482, 2001.
32. Miano, J.M.*, Thomas, S., and Disteche, C.M. Reversible expression and chromosomal mapping of the mouse
smooth muscle calponin gene. Mamm. Genome. 12:187-191, 2001.
33. Miano, J.M.*and Berk, B.C. Retinoids: New Insight into Smooth Muscle Cell Growth Inhibition. Editorial.
Arterioscler.Thromb.Vasc.Biol., 21:724-726, 2001.
34. Dulin, N.O., Orlov, S.N., Kitchen, C.M., Voyno-Yasenetskaya, T.A., and Miano, J.M.* G protein-coupled
receptor-activation of the smooth muscle calponin gene. Biochem.J., 357:587-592, 2001.
35. Chen, J., Streb, J.W., Maltby, K.M., Kitchen, C.M., and Miano, J.M.* Cloning of a novel retinoid-inducible
serine carboxypeptidase from vascular smooth muscle cells. J.Biol.Chem. 276:34175-34181, 2001.
36. Frame, M.D., Miano, J.M., Yang, J., and Rivers, R.J.* Localized adenovirus-mediated gene transfer into
vascular smooth muscle in the hamster cheek pouch. Microcirculation, 8:403-413, 2001.
37. Miano, J.M.*, Kitchen, C.M., Chen, J., Maltby, K.M., Kelly, L.A., Weiler, H., Krahe, R., Ashworth, L.K., and
Garcia, E. Human smooth muscle calponin expression in transgenic mice revealed with a bacterial artificial
chromosome. Am.J.Physiol. Heart Circ.Physiol., 282:H1793-1803, 2002.
38. Miano, J.M. Mammalian smooth muscle differentiation: origins, markers and transcriptional control. Results
Probl. Cell Differ. 38:39-59, 2002.
39. Chen, J., Kitchen, C.M., Streb, J.W., and Miano, J.M.* Myocardin: a component of a molecular switch for
smooth muscle cell differentiation. J.Mol.Cell.Cardiol., 34:1345-1356, 2002.
Editorialized in AB Firulli “Another hat for myocardin.” J.Mol.Cell.Cardiol., 34:1293-1296, 2002.
40. Miano, J.M. Cardiac-specific gene expression: a HANDful of factors. J.Mol.Cell.Cardiol., 34:1287-1291,
2002.
41. Streb, J.W. and Miano, J.M.* Retinoids: pleiotropic agents of therapy for vascular diseases? Curr. Drug
Targets – Cardiovasc.Haematol.Disord., 3:31-57, 2003.
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Joseph M. Miano, Ph.D.
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42. Davis, A., Hogarth, K., Fernandes, D., Solway, J., Niu, J., Kolenko, V., Luttrell, L., Browning, D., Miano,
J.M., Orlov, S., Dulin, N.O.* Functional significance of protein kinase A activation by endothelin-1 and ATP:
negative regulation of SRF-dependent gene expression by PKA. Cell.Signal., 15:597-604, 2003.
43. Chen, J., Chada, S., Mhashilkar, A., Miano, J.M.* Tumor Suppressor MDA-7/IL-24 Selectively Inhibits
Vascular Smooth Muscle Cell Growth and Migration. Molecular Therapy, 8:220-229, 2003.
44. Miano, J.M. Serum response factor: toggling between disparate programs of gene expression.
J.Mol.Cell.Cardiol., 35:577-593, 2003.
45. Aizawa, T., Wei, H., Miano, J.M., Abe, J-i., Berk, B.C., Yan, C*. Anti-inflammatory effects of NO/cGMP are
mediated by PDE3 Inhibition in vascular smooth muscle cell. Circ.Res., 93:406-413, 2003.
46. Jiang, M., Mezentsev, A., Kemp, R., Byun, K., Falck, J.R., Miano, J.M., Nasjletti, A., Abraham, N.G.,
Laniado-Schwartzman, M.* Smooth Muscle-Specific Expression of CYP4A1 Induces Endothelial Sprouting in
Renal Arterial Microvessels. Circ.Res., 94:167-174, 2004.
47. Zhang, R., Al-Lamki, R., Bai, L., Streb, J.W., Miano, J.M., Bradley, J., Min, W*. Thioredoxin-2 inhibits
mitochondria-located ASK1-mediated apoptosis in a JNK-independent manner. Circ.Res, 94:1483-1491, 2004.
48. Hogarth, D.K., Sandbo, N., Taurin, S., Kolenko, V., Miano, J.M., Dulin, N.O*. Dual role of PKA in
phenotypic modulation of vascular smooth muscle cells by extracellular ATP. Am.J.Physiol. Cell Physiol.
287:C449-C456, 2004.
49. Miano, J.M. Channeling to myocardin. Circ.Res. 95:340-342, 2004.
50. Miano, J.M.*, Ramanan, N., Georger, M.A., de Mesy Bentley, K.L., Emerson, R.L., Balza, R.O., Jr.,
Xiao, Q., Weiler, H., Ginty, D.D., Misra, R.P.* Restricted inactivation of serum response factor to the
cardiovascular system. Proc.Natl.Acad.Sci., USA., 101:17132-17137, 2004.
51. Streb, J.W., Kitchen, C.M., Gelman, I.H. Miano, J.M.* Multiple promoters direct expression of three
AKAP12 isoforms with distinct tissue and subcellular distribution profiles. J.Biol.Chem., 279:56014-56023,
2004.
52. Streb, J.W. and Miano, J.M.* AKAP12: An atypical serum response factor-dependent target gene.
J.Biol.Chem., 280:4125-4134, 2005.
53. Zlokovic, B.V.*, Deane, R., Sallstrom, J., Chow, N., Miano, J.M. Neurovascular pathways and Alzheimer
amyloid -peptide. Brain Pathol., 15:78-83, 2005.
54. Blaxall, B.C., Miano, J.M., Berk, B.C.* Angiotensin II: a devious activator of mineralocorticoid receptordependent gene expression. Circ.Res., 96:610-611, 2005.
55. Streb, J.W. and Miano, J.M.* Cross-species sequence analysis reveals multiple charged residue-rich domains
that regulate nuclear/cytoplasmic partitioning and membrane localization of A kinase anchoring protein 12
(SSeCKS/Gravin). J.Biol.Chem., 280:28007-28014, 2005.
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Joseph M. Miano, Ph.D.
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56. Ping, Q., Ritchie, R., Fu, Z., Cao, D., Cumming, J., Miano, J.M., Wang, D-Z., Li, H.J., Li, L.* Myocardin
enhances Smad3-mediated TGF-1 signaling in a CArG box-independent manner: Smad binding site (SBE)
is a critical cis-element for SM22 transcription in vivo. Circ.Res., 97:983-991, 2005.
57. Sun, Q., Chen, G., Streb, J.W., Long, X, Yang, Y., Stoeckert, C.J., Jr., Miano, J.M.* Defining the
mammalian CArGome. Genome Res., 16:197-207, 2006.
58. Rensen, S.S.M., Niessen, P.M.G., Long, X., Doevendans, P.A., Miano, J.M., van Eys, G.J.J.M. * Contribution
of serum response factor and myocardin to transcriptional regulation of smoothelins. Cardiovasc.Res., 70:136145, 2006.
59. Lee, T-H.D., Streb, J.W., Georger, M.A., Miano, J.M. * Tissue expression of the novel serine
carboxypeptidase Scpep1. J.Histochem.Cytochem., 54:701-711, 2006.
60. Nagel, D.J., Aizawa, T., Jeon, K-I., Liu, W., Mohan, A., Wei, H., Miano, J.M., Florio, V.A., Gao, P.,
Korshunov, V.A., Berk, B.C., Yan, C. Role of nuclear Ca2+/calmodulin-stimulated phosphodiesterase 1A in
vascular smooth muscle cell growth and survival. Circ.Res., 98:777-784, 2006.
61. Miano, J.M. Fishing for function in zebrafish. Circ.Res., 98:723-726, 2006.
62. Miano, J.M.*, Georger, M.A., Rich, A., de Mesy Bentley, K.L. Ultrastructure of zebrafish dorsal aortic cells.
Zebrafish, 3:455-463, 2006.
63. Miano, J.M.*, Berk, B.C. HDAC7 supports vascular integrity (Ed). Nat.Med. 12:997-998, 2006.
64. Miano, J.M.*, Long, X.C., Fujiwara, K. Serum response factor: master regulator of the actin cytoskeleton and
contractile apparatus. Am.J.Physiol. Cell Physiol., 292:C70-C81, 2007.
65. Chow, N., Bell, R.D., Deane R., Streb, J.W., Guo, H., Chen, J., Rubio, A., Brooks, A., Van Nostrand, W.,
Miano, J.M.*, and Zlokovic, B.V.* Serum response factor and myocardin mediate arterial hypercontractility
and cerebral blood flow dysregulation in Alzheimer’s phenotype. Proc.Natl.Acad.Sci., USA., 104:823-828,
2007. (*Co-senior authorship).
66. Long, X. and Miano J.M.* Remote control of gene expression. J.Biol.Chem., 282:15941-15945, 2007.
67. Long X., Creemers, E.E., Wang, D-Z., Olson, E.N. and Miano, J.M.* Myocardin is a bifunctional switch for
smooth versus skeletal muscle differentiation. Proc.Natl.Acad.Sci., USA., 104:16570-16575, 2007.
68. Davis, J.L., Long, X., Georger, M.A., Scott, I.C., Rich, A, and Miano, J.M.* Expression and comparative
genomics of two serum response factor genes in zebrafish. Int.J.Dev.Biol., 52:389-396, 2008.
69. Long, X., Bell, R.D., Gerthoffer, W.T., Zlokovic, B.V., and Miano, J.M.* Myocardin is sufficient for a
smooth muscle cell-like contractile phenotype. Arterioscler.Thromb.Vasc.Biol., 28:1505-1510, 2008.
70. Miano, J.M. SRF’ing the actin cytoskeleton with no destrin. Physiol. Genomics., 34:6-8, 2008.
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71. Miano, J.M. Deck of CArGs. Circ.Res., 103:13-15, 2008.
72. Bell, R.D., Deane, R, Chow, N., Long, X., Sagare, A., Streb, J.W., Guo, H., Rubio, A., Van Nostrand, W.,
Miano, J.M.,* and Zlokovic, B.V.* SRF and myocardin repress LRP-mediated amyloid-beta clearance in brain
vascular smooth muscle cells via SREBP2. Nat.Cell.Biol., 11(2):143-153, 2009.
73. Cordes, K.R., Sheehy, N.T., White, M., Berry, E., Morton, S.U., Muth, A.N., Lee, T-H., Miano, J.M., Ivey,
K.N., and Srivastava, D. miR-145 and miR-143 regulate smooth muscle cell fate and plasticity. Nature,
460:705-710, 2009.
74. Lee, T-H., Chen, J., Miano, J.M. Functional characterization of a putative serine carboxypeptidase in vascular
smooth muscle cells. Circ.Res, 105:271-278, 2009.
75. Sun, Q., Taurin, S., Wang D.Z., Zimmer W.E., Dulin N., Miano J.M. Myocardin-Dependent Activation of the
CArG Box-Rich Smooth Muscle Gamma Actin Gene: Preferential Utilization of a Single CArG Element
through Functional Association with the NKX3.1 Homeodomain Protein. J.Biol.Chem. In revision.
76. Long, X., Tharp, D.L., Georger, M.A., Slivano, O.J., Lee, M.Y., Wamhoff, B.R., Bowles, D.K., Miano, J.M.
The smooth muscle cell-restricted KCNMB1 ion channel subunit is a direct transcriptional target of serum
response factor and myocardin. J.Biol.Chem. Submitted.
77. Albinsson, S., Suarez,Y., Miano, J.M., Sessa, W.C. miRNAs are necessary for vascular smooth muscle
growth and differentiation during development. Proc.Natl.Acad.Sci., USA., Submitted.
XX. Selected Abstracts
1. Starzec, J.J., Berger, D.F., Corrao, R.M. and Miano, J.M. Serum cholesterol levels and daily duration of
swimming stress in rats. Eastern Psychological Association, Boston, MA, 1984 (poster).
2. Mettler, N.R., Miano, J.M., Wang, W.H. and Kikkawa, Y. Secretory factors from phagocytosing alveolar
macrophages depresses cytochrome P-450 in isolated hepatocytes. Federation Of American Societies Of
Experimental Biology (FASEB) J. 3:A1601, 1988 (poster).
3. Miano, J.M., Starzec, J.J. and Berger, D.F. The effects of voluntary exercise on diet-induced alterations in
blood lipids and aortic histopathology in the rate. Eastern Psychological Association, Boston, MA, 1989
(poster).
4. Miano, J.M., Tota, R.R., Vlasic, N. and Stemerman, M.B. Early induction of c-myc following balloon injury
to the rat aorta. Fed. of Am. Soc. Exp. Biol. (FASEB) J. 5:A480, 1990 (oral presentation).
5. Tota, R.R., Vlasic, N., Miano, J.M. and Stemerman, M.B. Vascular smooth muscle cell proto-oncogene
induction by physical manipulation. Arteriosclerosis 10:844a, 1990.
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6. Miano, J.M., Vlasic, N., Tota, R.R., O'Banion, M.K., Young, D.A. and Stemerman, M.B. Immediate early
gene (IEG) activation in aortic rat smooth muscle cells following vascular injury: Relationship to in vivo
growth factor expression and DNA synthesis. Circulation 84:II,342, 1991 (oral presentation).
7. Tota, R.R., Miano, J.M., Vlasic, N. and Stemerman, M.B. Role of immediate early genes (IEG) in autocrine
growth of rat aortic smooth muscle cells (SMC) in vivo following vascular injury. J. Cell. Biochem. 16A:13,
1992 (poster).
8. Giachelli, C.M., Miano, J.M., Olson, E.N., Firulli, A.B., Hara, P.S., Soderling, S. and Schwartz, S.M.
Homeobox gene expression distinguishes human fetal and adult aortic smooth muscle cells in culture.
Federation of American Societies Of Experimental Biology (FASEB) Journal. 8:A264, 1994 (poster).
9. Miano, J.M., Cserjesi, P., Li, L., Ligon, K.L. and Olson, E.N. Specificity of smooth muscle cell markers in
adult and developing mouse tissues. American Heart Association Meeting, November, 1994.(poster).
10. Miano, J.M., Topouzis, S., Majesky, M.W. and Olson, E.N. Smooth muscle cells express functional retinoid
receptors and are negatively growth regulated by all-trans retinoic acid. Keystone Symposium, February, 1996
(poster).
11. Weihrauch, D., Chilian, W.M., Tessmer, J., Miano, J.M., and Warltier, D.C. Intermittent myocardial ischemia
results in the expression of mitogens in the cardiac interstitium. American Heart Association Meeting,
November, 1996 (poster).
12. Broeckel, U., Jiang, J., Tonellato, P., Feroah, T., Jacob, H.J. and Miano, J.M. Assessing the spatial
relationship between functional units of genes as a tool for defining genes from genomic sequence with
bioinformatics and bioassays. Cold Spring Harbor Genome Mapping and Sequencing Meeting. May 14-18,
1997 (poster)
13. Miano, J.M., Roloff, L.A., Piantedose, R., Blaner, W.S., and Kipshidze, N.N. Systemic administration of
retinoic acid reduces intimal thickening following balloon injury in a rat model. Transcatheter Cardiovascular
Therapeutics IX, Washington, DC, September 24-26, 1997 (Poster).
14. Konorev, E.A., Miano, J.M., and Kalyanaraman, B. Bicarbonate enhances doxorubicin-induced myocardial
damage via a superoxide radical-dependent mechanism. AHA Meeting, November, 1998 (Poster).
15. Foschi, M., Barboni, E., Miano, J.M., Vandewalle, W., LaVilla, G., Gentilini, P., Franchi, F. Adenoviralmediated gene delivery targeted to hepatic stellate cells. Hepatology. 28:520A, 1998 (Poster).
16. Miano, J.M. Transcriptional control of smooth muscle calponin. Federation of American
societies of experimental biology (FASEB), April 18, 1999 (oral).
17. Miano, J.M., Foschi, M., Friesel, R., and Chada, S. Vascular smooth muscle cell-specific gene targeting with
the SM22 promoter. SCVIR, March 25, 2000.
18. Frame, M.D., Miano, J.M., Rivers, R.J. Technique for targeting adenoviral constructs to microcirculatory
vascular smooth muscle, in vivo. FASEB, March, 2001.
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19. Streb, J.W., Chen, J. Miano, J.M. Novel expression of a tumor suppressor gene in vascular smooth muscle
cells following retinoid administration. ATVB, May 11-13, 2001. (*NAVBO’s Servier New Investigator
Award to Jeffrey Streb*)
20. Chen, J., Streb, J.W. Miano, J.M. Cloning of a novel retinoid-response gene in vascular smooth muscle cells.
ATVB, May 11-13, 2001.
21. Miano, J.M., Kitchen, C.M., Chen, J., Wang, D-Z., Olson, E.N. Myocardin is expressed in adult aorta and
activates a smooth muscle cell differentiation program. ATVB/NAVBO, April 6, 2002 (oral).
22. Streb, J.W., Georger, M.A., Miano, J.M. Retinoids activate cyclic AMP response element binding protein in
vascular smooth muscle cells via protein kinase A. ATVB/NAVBO, April 6, 2002.
23. Georger, M.A., Kitchen, C.M., Miano, J.M. Human smooth muscle calponin protein is tightly restricted to
smooth muscle cell lineages in transgenic mice. ATVB/NAVBO, April 7, 2002.
24. Kitchen, C.M., Chen, J., Streb, J.W., Miano, J.M. VISTA and PIP analyses reveal unconventional regulatory
domains in a vascular smooth muscle cell-restricted, retinoid-inducible integrin subunit. ATVB/NAVBO,
April 7, 2002.
25. Miano, J.M. Mining BACs for regulatory elements controlling human smooth muscle calponin expression in
transgenic mice. Cold Spring Harbor LXVII Symposium on the Cardiovascular System, May 30, 2002.
26. Sun, Q., Chen, G., Streb, J.W., Yang, Y., Stoeckert, C., Miano, J.M. Defining the mammalian CArGome.
Cold Spring Harbor Laboratory, Systems Biology: Global Regulation of Gene Expression. March 18, 2005.
27. Chen, G.G., Miano, J.M., Jensen, S.T., Stoeckert, C. Identify functional regulons by integrating
heterogeneous data with Bayesian hierarchical modeling. Systems Biology: Global Regulation of Gene
Expression. March 24, 2006.
28. Wang, L., Miller, C., Rao, M.H., Miano, J.M., Mackman, N., Taubman, M.B. Vascular smooth musclederived tissue factor is critical for arterial thrombosis after Fecl3 injury. ATVB 27:E47-E48, 2007 (poster).
29. Lee, T.H. and Miano, J.M. Functional analysis of a novel serine carboxypeptidase. ATVB Annual
Conference. April 18, 2008 (poster).
30. Long, X., Hyu, C., Miano, J.M., Chen, Y. Mutual regulation of Runx2 and Myocardin during oxidative stressinduced smooth muscle cell phenotypic modulation. ATVB Annual Conference. April 17, 2008 (poster).
31. Long, X., Bell, R.D., Zlokovic, B.V., Miano, J.M. Initial characterization of the Myocardin promoter and its
regulation by hypoxia. ATVB Annual Conference. April 18, 2008 (poster).
32. Long, X., Bell, R.D., Zlokovic, B.V., Miano, J.M. Myocardin is sufficient for the smooth muscle cell
contractile phenotype. ATVB Annual Conference. April 18, 2008 (oral, Long).
Curriculum Vitae
July 28, 2009
Joseph M. Miano, Ph.D.
Page 21
XXI. Patents
1. Olson, E.N., Li, L., and Miano, J.M. “Smooth muscle 22 promoter, gene transfer vectors containing the
same, and method of use of the same to target gene expression in arterial smooth muscle cells.” Patents Issued
November 17, 1998 (US Patent No. 5,837,534) and January 18, 2000 (US Patent No. 6,015,711). Licensed to
AmGen.
2. Miano, J.M., Streb, J.W., and Chen, J. “Retinoid-inducible proteins of vascular smooth muscle cells and uses
thereof.” Patent filed February 22, 2002. Nixon Peabody Reference No. 176/61021.
3. Streb, J.W. and Miano, J.M. “Recombinase-Mediated Transcription” Provisional Patent filed November 7,
2002. Needle & Rosenberg. U.S. Application No. 10/533,976; International Application No.
PCT/US03/035645.
4. Zlokovic, B.V. and Miano, J.M. “Serum response factor and myocardin control Alzheimer cerebral amyloid
angiopathy. Provisional Patent Filed November 1, 2005. Nixon & Vanderhye. U.S. Serial No. 60/735,965.