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Conditioned Emotional Response Lab Report Focus on Method Abstract Introduction Summarize lab introduction for Conditioned Emotional Response or Fear Conditioning Rationale: why do this lab exercise? Brief description of procedures as carried out in this exercise Hypothesis: What changes in behavior were expected as a result of presenting a CS paired with a US? *Method* Subject Rats are male, albino rats Provide number, age and weights of rats at time of observation; DOB 7/28/12 Provide information about light/dark cycle in vivarium, feeding, and watering Previous experience with conditioning Apparatuses Chamber A Used MED-Associates standard rat operant chamber with water dipper assembly with 0.01 cc cup Constructed of aluminum, stainless steel, polycarbonate Double lever, house light, 2 stimulus lights (provide locations) Push Rod in ceiling Chamber B Plastic opaque rat cage with wire lid Chambers were controlled by MED-Associates' software, MED-PC IV, on a Dell Pentium D PC running Windows XP Used MED-Associates Shocker Scrambler Module, ENV-414 Shock: 1.5mA, 1.0 s foot shock Used a MED-Associates tone generator Tone: 80dB, 5000Hz, 20 s. Procedure Describe important steps in sequence for replicating fear conditioning procedure May summarize lab instructions but do not plagiarize Provide criterion if used one Results Graph 1--mean percentage of time that animal froze during Acclimation, Immediate and Delayed Testing as a function of conditioning trials Describe verbally what data in the graphs show Discussion Summarize results in the context of hypothesis Relate results to rationale in introduction Describe limitations of study Future directions; next study; re-do of study Procedures adapted from: LaBar, K. S., & LeDoux, J. E. (1996). Partial disruption of fear conditioning in rats with unilateral amygdala damage: Correspondence with unilateral temporal lobectomy in humans. Behavioral Neuroscience, 110, 991-997. doi: 10.1037/0735-7044.110.5.991 Schafe, G. E., & Ledoux, J. E. (2000). Memory consolidation of auditory Pavlovian fear conditioning requires protein synthesis and protein kinase A in the amygdala. The Journal of Neuroscience, 20(18), RC96. doi: 10.1016/S01662236(00)01969-X Retrieved from http://www.jneurosci.org/content/20/18/RC96.full.pdf+html?sid=420c4788-42304418-b6b9-4c6aafdc5815 Schafe, G. E., Nadel, N. V., Sullivan, G. M., Harris, A., & Ledoux, J. E. (1999). Memory consolidation for contextual and auditory fear conditioning is dependent on protein synthesis, PKA, and MAP kinase. Learning and Memory, 6, 97110.