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Supplemental Information
Table 1. Left ventricular size and function in MCM;Myc+/+ and MCM;Mycfl/fl mice after 2
weeks of Sham or TAC
MCM;Myc+/+
MCM;Mycfl/fl
Sham
TAC
Sham
TAC
6
6
6
6
HR (bpm)
506+20
540+29
502+36
527+17
ESD (mm)
1.75+0.15
1.95+0.24
1.68+0.14
1.80+0.15
EDD (mm)
3.18+0.12
3.21+0.18
3.20+0.12
3.13+0.17
IVST (mm)
0.69+0.06
0.93+0.08*
0.72+0.03
0.79+0.04
PWT (mm)
0.65+0.06
0.81+0.1
0.68+0.06
0.69+0.08
AoEt (ms)
55.8+3.2
59.4+1.3
56.7+2.0
57.6+1.9
LV %FS
45.3+3.2
40.5+3.7
47.8+2.4
42.7+2.7
Vcf (mm/s)
8.29+0.84
6.81+0.61
8.49+0.60
7.49+0.61
LVM (mg)
60.2+5.8
88.1+8.5*
64.6+4.1
68.3+8.2
n
Echocardiographic analysis of the left ventricle: HR, heart rate; ESD, end-systolic diameter; EDD, end-diastolic
diameter; IVST, intraventricular septal thickness; PWT, posterior wall thickness; AoET, aortic ejection time; LV%FS, left
ventricular fractional shortening; Vcf, circumferential shortening velocity; LVM, calculated left ventricular mass.
Results presented as mean+S.E.M., ANOVA *p<0.05 versus Sham animals
Table 1 – 2-D echocardiograms to assess LV function were performed on 4-OHT-treated control
MCM:Myc+/+ or MCM:Mycfl/fl mice after two weeks of Sham or TAC surgery.
2-D Echo studies. Echocardiography on transgenic mice was performed as described (Jordan et
al, 2005). Estimation of left ventricular mass has been described (Gardin et al, 1995). At least
three independent M-mode measurements per animal were obtained by an examiner blinded to
the genotype of the animal.
References
Gardin JM, Siri FM, Kitsis RN, Edwards JG, Leinwand LA (1995) Echocardiographic
assessment of left ventricular mass and systolic function in mice. Circ Res 76: 907-914.
Jordan MC, Zheng Y, Ryazantsev S, Rozengurt N, Roos KP, Neufeld EF (2005) Cardiac
manifestations in the mouse model of mucopolysaccharidosis I. Mol Genet Metab 86: 233-243.