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KING ABDUL AZIZ UNIVERSITY
FACULTY OF SCIENCE
Department of Biology
Course Title & Code: GENERAL MICROBIOLOGY (BIO 231)
Student's Name: ……………………………………………………………
UID: …………………… Date …………………. Duration: ……………..
First Periodical Exam
1.1 Multiple Choice Questions( 10 marks)
1- Which of the following statements is the best definition of
biogenesis?
A) Nonliving matter gives rise to living organisms.
B) Living cells can only arise from preexisting cells.
C) A vital force is necessary for life.
D) Air is necessary for living organisms.
E) Microorganisms can be generated from nonliving matter.
2- The term used to describe a disease-causing microorganism is
A) microbe.
B) bacterium.
C) virus.
D) pathogen.
E) infection.
3-Which of the following is not a characteristic of bacteria?
A) are prokaryotic
B) have peptidoglycan cell walls
C) have the same shape
D) grow by binary fission
E) have the ability to move
4-Which of the following is NOT associated with viruses?
A) organelles
B) nucleic acid
C) envelope
D) spikes
E) capsid
5- Which of the following statements about E. coli is not true?
A) E. coli was the first disease-causing bacterium identified
by Koch.
B) E. coli is part of the normal microbiota of humans.
C) E. coli is beneficial in human intestines.
D) A disease-causing strain of E. coli causes bloody diarrhea.
E) none of the above
6- The infectious agent that causes AIDS is a
A) virus.
B) bacterium.
C) yeast.
D) protozoan.
E) mold.
7-Archaea differ from bacteria in that archaea
A) have diverse cell wall compositions.
B) lack nuclei.
C) use organic compounds for food.
D) reproduce by binary fission.
E) are prokaryotic.
8- Who is credited with first observing cells?
A) Robert Hooke
B) Anton van Leeuwenhoek
C) Robert Koch
D) Louis Pasteur
E) Carolus Linnaeus
9- Biogenesis refers to the
A) spontaneous generation of organisms from nonliving matter.
B) development of life forms from preexisting life forms.
C) development of aseptic technique.
D) germ theory of disease.
10- The microbial process of converting sugars to alcohol is known as
A) fermentation.
B) pasteurization.
C) tyndallization.
D) lyophilization.
E) alcoholism.
11-Which of the following statements is INCORRECT regarding prokaryotic cells?
A) Their DNA is not enclosed within a membrane.
B) They lack membrane-enclosed organelles.
C) They typically have a circular chromosome.
D) They reproduce by binary fission.
E) They lack a plasma membrane.
12- A gram-positive bacteria suddenly acquires resistance to the antibiotic methicillin.
This trait most likely occurred due to
A) conjugation.
B) binary fission.
C) meisosis.
D) transformation.
E) transduction.
13- Which of the following statements best describes what happens when a bacterial cell
is placed in a solution containing 5 percent NaCl?
A) Sucrose will move into the cell from a higher to a lower concentration.
B) The cell will undergo osmotic lysis.
C) Water will move out of the cell.
D) Water will move into the cell.
E) No change will result; the solution is isotonic
14-) The DNA found in most bacterial cells
A) is surrounded by a nuclear membrane.
B) utilizes histones for chromosomal packaging.
C) is circular in structure.
D) is linear in structure.
E) is found in multiple copies.
15-Which diagram represents a gram-positive bacterium? How can
you tell?
16-By which of the following mechanisms can a cell transport a substance from a lower
to a higher concentration?
A) simple diffusion
B) facilitated diffusion
C) active transport
D) extracellular enzymes
E) aquaporins
17- Which of the following statements is TRUE?
A) Endospores are for reproduction.
B) Endospores allow a cell to survive environmental changes.
C) Endospores are easily stained in a Gram stain.
D) A cell produces one endospore and keeps growing.
E) A cell can produce many endospores.
18-Which of the following pairs is mismatched?
A) endoplasmic reticulum internal transport
B) Golgi complex secretion
C) mitochondria ATP production
D) centrosome food storage
E) lysosome digestive enzymes
Figure 4.1
19- Which drawing in Figure 4.1 possesses an axial filament?
A) a
B) b
C) c
D) d
E) e
20-Functions of the glycocalyx include all of the following EXCEPT
A) biofilm formation.
B) increased virulence.
C) source of nutrition.
D) protection against dehydration.
E) binary fission.
2.2 True/False Questions( 5 marks)
1-The process of pasteurization to reduce food spoilage utilizes high heat to kill all
bacteria present.(
)
2- Anton van Leeuwenhoek was the first microbiologist to use a microscope to examine
environmental samples for the presence of microorganisms.(
)
3-Microbes are associated with life-sustaining benefits as well as life-threatening
diseases.(
)
4- All cells possess a cell wall.(
)
5- Some viruses can contain both DNA and RNA.(
)
6- Antibiotics that target the cell wall are an effective treatment against many pathogenic
bacteria.(
)
7- Endospores are a reproductive structure.(
)
8- Small, hydrophobic molecules pass through a cell membrane most easily by diffusion.
(
)
9- The internal structure of eukaryotic cilia and flagella are the same.(
)
10- The DNA found in most bacterial cells is found in multiple copies.(
Good Luck
)
KING ABDUL AZIZ UNIVERSITY
FACULTY OF SCIENCE
Department of Biology
Course Title & Code: GENERAL MICROBIOLOGY (BIO 231)
Student's Name: ……………………………………………………………
UID: …………………… Date …………………. Duration: ……………..
First Periodical Exam ( Model Answer )
1.1 Multiple Choice Questions( 10 marks)
1- Which of the following statements is the best definition of
biogenesis?
A) Nonliving matter gives rise to living organisms.
B) Living cells can only arise from preexisting cells.
C) A vital force is necessary for life.
D) Air is necessary for living organisms.
E) Microorganisms can be generated from nonliving matter.
2- The term used to describe a disease-causing microorganism is
A) microbe.
B) bacterium.
C) virus.
D) pathogen.
E) infection.
3-Which of the following is not a characteristic of bacteria?
A) are prokaryotic
B) have peptidoglycan cell walls
C) have the same shape
D) grow by binary fission
E) have the ability to move
4-Which of the following is NOT associated with viruses?
A) organelles
B) nucleic acid
C) envelope
D) spikes
E) capsid
5- Which of the following statements about E. coli is not true?
A) E. coli was the first disease-causing bacterium identified
by Koch.
B) E. coli is part of the normal microbiota of humans.
C) E. coli is beneficial in human intestines.
D) A disease-causing strain of E. coli causes bloody diarrhea.
E) none of the above
6- The infectious agent that causes AIDS is a
A) virus.
B) bacterium.
C) yeast.
D) protozoan.
E) mold.
7-Archaea differ from bacteria in that archaea
A)have diverse cell wall compositions.
B) lack nuclei.
C) use organic compounds for food.
D) reproduce by binary fission.
E) are prokaryotic.
8- Who is credited with first observing cells?
A) Robert Hooke
B) Anton van Leeuwenhoek
C) Robert Koch
D) Louis Pasteur
E) Carolus Linnaeus
9- Biogenesis refers to the
A) spontaneous generation of organisms from nonliving matter.
B) development of life forms from preexisting life forms.
C) development of aseptic technique.
D) germ theory of disease.
10- The microbial process of converting sugars to alcohol is known as
A)fermentation.
B) pasteurization.
C) tyndallization.
D) lyophilization.
E) alcoholism.
11-Which of the following statements is INCORRECT regarding prokaryotic cells?
A) Their DNA is not enclosed within a membrane.
B) They lack membrane-enclosed organelles.
C) They typically have a circular chromosome.
D) They reproduce by binary fission.
E) They lack a plasma membrane.
12-A gram-positive bacteria suddenly acquires resistance to the antibiotic methicillin.
This trait most likely occurred due to
A) conjugation.
B) binary fission.
C) meisosis.
D) transformation.
E) transduction.
13- Which of the following statements best describes what happens when a bacterial cell
is placed in a solution containing 5 percent NaCl?
A) Sucrose will move into the cell from a higher to a lower concentration.
B) The cell will undergo osmotic lysis.
C) Water will move out of the cell.
D) Water will move into the cell.
E) No change will result; the solution is isotonic
14-) The DNA found in most bacterial cells
A) is surrounded by a nuclear membrane.
B) utilizes histones for chromosomal packaging.
C) is circular in structure.
D) is linear in structure.
E) is found in multiple copies.
15-Which diagram represents a gram-positive bacterium? How can
you tell?
Diagram (a ) refers to a gram-positive bacterium because the lipopolysaccharidephospholipids- lipoprotein layer is absent.
Or Diagram ( a) becauseit has a thick peptidoglycan layer and teichoic acid
16-By which of the following mechanisms can a cell transport a substance from a lower
to a higher concentration?
A) simple diffusion
B) facilitated diffusion
C)active transport
D) extracellular enzymes
E) aquaporins
17- Which of the following statements is TRUE?
A) Endospores are for reproduction.
B) Endospores allow a cell to survive environmental changes.
C) Endospores are easily stained in a Gram stain.
D) A cell produces one endospore and keeps growing.
E) A cell can produce many endospores.
18-Which of the following pairs is mismatched?
A) endoplasmic reticulum internal transport
B) Golgi complex secretion
C) mitochondria ATP production
D) centrosome food storage
E) lysosome digestive enzymes
Figure 4.1
19- Which drawing in Figure 4.1 possesses an axial filament?
A) a
B) b
C) c
D) d
E) e
20-Functions of the glycocalyx include all of the following EXCEPT
A) biofilm formation.
B) increased virulence.
C) source of nutrition.
D) protection against dehydration.
E) binary fission
2.2 True/False Questions( 5 marks)
1-The process of pasteurization to reduce food spoilage utilizes high heat to kill all
bacteria present.(X)
2- Anton van Leeuwenhoek was the first microbiologist to use a microscope to examine
environmental samples for the presence of microorganisms.(√)
3-Microbes are associated with life-sustaining benefits as well as life-threatening
diseases.(√)
4- All cells possess a cell wall.(X)
5- Some viruses can contain both DNA and RNA.(X)
6- Antibiotics that target the cell wall are an effective treatment against many pathogenic
bacteria.(√)
7- Endospores are a reproductive structure.
.
(X)
8- Small, hydrophobic molecules pass through a cell membrane most easily by diffusion.
(√)
9- The internal structure of eukaryotic cilia and flagella are the same.(√)
10- The DNA found in most bacterial cells is found in multiple copies.(X)
Good Luck