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1 Bacteria Define of bacteria: Bacteria are unicellular microscopic organism and have a cell wall. Some bacteria have flagella ,fimbria, capsule and spore. It contain nucleus no unclear membrane and nucleoli. They are multiply by binary fission. Classification of bacteria: 1.Cocci. A)Gram positive Cocci. i)Strepto coccus ii)Staphylo coccus iii)Pneumo coccus B)Gram negative Cocci. i)Neisseria gonorrhoae ii)Neisseria meningitides 2)Bacilli. A)Gram positive bacilli. i)Clostridium. (Tetani,Botulism) ii)Corynebacterium diphtheria. iii)Bacillus anthrax B)Gram negative bacilli. i)Escherechia coli ii)Salmonella Typhi iii)Vibrio cholera iv)Proteus Vulgaris C)Acid first bacillus i)Mycobacterium tuberculosis ii)Mycobacterium leprae D)Flexible Spirochetes. i)Leptospiral ii)Treponema E)Mycelial Bacteria. i)Streptomyces ii)Actinomyces A. Morphological classification 1. Cocci: they are rounded. On the basis of their arrangement they are further classified into i) Monococcus: They remain single. ii) Diplococcus: They remain double. iii) Streptococcus; They remain in chain form. iv) Staphylococcus: They remain in cluster. 2. Bacilli: They are elongated rod like bacteria, e.g.- E. coli. 3. Spirochaetes: They are spiral in shape, e.g.- Treponenta pelidutn. 4. Vibrio: They are comma-shaped, e.g. - Vibrio cholerae. 2 B. On the basis of gram stain: i. Gram positive bacteria. Example — Steptococcus, Clostridium (Tetani, weichi) ii. Gram negative bacteria. Example — Neisseria gonoriae, E. coli, Salmonella typhi. C. On the basis of acid fast staining (i) Acid fast. Example — Mycobacterium tuberculosis. (ii) Non acid fast. Example — Streptococcus. D. On the basis of oxygen requirement: Bacteria can be into following groups on O2 requirement: i. Obligate aerobe. Example Pseudomonas, Mycobacterium tuberculosis. ii. Facultative anaerobe: Grows with or without O2 Example Staphylococci, E.Coli. iii. Obligate- anaerobe: Grows only in absence of O2. They cannot live in the presence of O2. Example Clostridium species. iv. Micro-aerophilic: Grow in low oxygen concentration Example Helicobacter pylori, Campylobacter jejuni. On the basis of O2 requirement are divided in to 4 type1. Facultative anaerobe:- Most of the bacteria can grow both in aerobic and anaerobic condition. 2. Strict anaerobe:- They can not survive in presence of O2. Ex Clostridium tetani 3. Strict aerobe:- They can not survive without O2. Ex Mycobacterium tuberculosis 4. Microaerophils:- Growing at low amount of O2 E. On the basis of spore formation. (i) Spore forming bacteria. Example — Clostridium species, Bacillus species. (ii) Non-spore forming bacteria. Example — Staphylococcus aureus, streptococcus pyogenes F. On the basis of temperature: (i) Psychrophilic Bacteria: Grow best at low temperature (15-20°C) (ii) Mesophilic bacteria: Grow best at body temperature (30-37°C) (ii) Thermophilic bacteria: Grow best at high temperature (50-60°C) G. On the basis of Capsule: i. Capsulated. Ex. Staphylococcus ii. Non-capsulated bacteria. Ex. Neisseria H. On the basis of Motile: 3 i. Motile bacteria. Ex- Escherichia coli ii. Non-motile bacteria. Ex- Shigella I. On the basis of Toxicity: i. Exotoxin. Ex- Streptococcus, Staphylococcus ii. Endotoxin: Ex- Vibrio cholera, E.coli J. On the basis of flagella: i. flagellated bacteria ii. Non flagellated bacteria K. On the basis of Cell Wall: i. Lacking cell wall- Mycoplasma and Ureaplasma, ii. Having cell wall- All other bacteria of medical importance L. On the basis of Habitat: i. Obligate intracellular- Rickettsia, Coxiella and Chiamydia. ii. Free-living- All other bacteria of medical importance. M. On the basis of Shape: i. Cocci- Spherical (most), lanceolate or bean shaped, ii. Bacilli (rods)- Straight, curved, helical or spiral.