Download Green fluorescent protein (gfp) gene inserted into the mouse genome

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Transcript 
Finish chick early development:
Node regression, notochord formation, and
neurulation in the ectoderm
Organization of the body axis, cross-section
Development of 4 extra-embryonic tissues
Regression of Hensen’s Node:
1) progressively laying down notochord toward the
posterior tip
and
2) progressively shutting down endomesoderm
induction and waterfall ingression thru the streak.
N
N
N
Stage 4
18hr
Stage 5
Stage 6
N
Stage 7
N
N
Stage 7
Stage 8
Regression of Hensen’s Node
N
Stage 9
N
Stage 10 36hr
Neurulation in
the chick,
after the node
regresses
Typical vertebrate organization of the chick embryo
(and >200 selector gene domains)
“Somatic mesoderm”
“Visceral mesoderm”
Notochord
**Or wait until the
embryo lifts
off the yolk sac
Extra-embryonic membranes of the chick
Amnion and chorion: contain ectoderm and
somatic mesoderm
Yolk sac and allantois: contain enododerm and
visceral mesoderm
Chick development: Formation of the allantois
Start mouse development:
fertilization to the 128 cell
blastocyst
Human or mouse embryo: pre-implantation stages
2. 2Blastocyst cavity formation
3. Cell sorting (hypo vs. epi)
4. hatching
128 cell
Day 7-human
Day 5.5-mouse
1. compaction
gene expression starts
Fallopian
tube
128-cell blastocyst
compaction
128-cell blastocyst
Mouse pre-implantation stages:
compaction, apical-basal polarization,
cleavage, blastocyst cavity formation
Compaction at the late 8-cell stage,
With apical-basal polarization of cells
Human 128cell blastocyst before/during hatching
Oct4 gene expression in epiblast
“zona”
Holding pipet
Injection pipet
Injection of
ES cells into the
blastocyst cavity
Mouse embryonic stem cells (ES cells)
from the inner cell mass, growing in a culture dish
Dr. Mario
Capecchi-1984
Univ. of Utah
(random)
(e.g., GFP
with promoter)
Mouse transgenesis (insertion of a
foreign gene into the mouse genome)
non-homologous integration
FOSTER
MOTHER
(2x)
Green ES cells
Gene for red fluorescent
protein (RFP)
Gene for green fluorescent
protein (GFP) from jellyfish
Implantation of the human blastocyst in the uterine wall.
Mouse implantation is also invasive, like human
Syncytiotrophoblast
Maternal
tissue
Uterine
fluid
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