Download manipulation of whole blood using traveling wave dielectrophoresis

MANIPULATION OF WHOLE BLOOD
USING TRAVELING WAVE
DIELECTROPHORESIS
Y.J. Lo, A.M. Wo, and U. Lei
Institute of Applied Mechanics, National Taiwan University,
Taipei, Taiwan
(MEMS2005 P.686~689)
Reporter: Chung-Kuang Chin(秦重光)
Date:10/24/2007
Outline





Motivation
Introduction
Working Theory of DEP/twDEP
Fabrication
Experiment Results
Motivation

Beautiful Life, Money－＞Health Care
－＞Bio-Medical Science + MEMS
－＞Bio-MEMS
( “Lab on a Chip”，”Micro-total analysis system” )
－＞cell manipulation (μm)
－＞DEP force (working on whole blood is rare)
－＞This paper !!!!
(MANIPULATION OF WHOLE BLOOD USING TRAVELING WAVE
DIELECTROPHORESIS )
－＞But after Reading，…@#&%*!
Ref: http://www.imm-mainz.de/upload/bilder/d137027ad17056118590d74d1c2b8b30.orig.jpg
Introduction

DEP－conventional DEP (cDEP) 、rotation DEP (rotDEP) 、traveling-wave
DEP (twDEP)
10MHz
Theory－DEP/twDEP

DEP force
－factor：non-uniform electric field、electrical properties of particles & medium
Ref: http://www.ibmm.informatics.bangor.ac.uk
Theory Formula
DEP force


F  t   2 r 3 m Re  K *   E 2rms  Im  K *   Ex 2 x  Ey 2 y  Ez 2 z
(
K *   
 p m
*
*
 p  2 m
*

*
ε：permittivity ，
，Clausius–Mossotti factor
p

 m*   m  j m
 p*   p  j ，


σ：conductivity ， ω：angular frequency ， r：particle radius
E rms：rms value of the electric field strength
E i ，  i (i=x,y,z)：magnitude ，phase
)
Real part－＞(Conventional) DEP
，
，
Imaginary part－＞twDEP
Clausius-Mossotti factor
K *   
 p*   m*
 p  2 m
*
*
( induced dipole moment factor)
Clausius-Mossotti factor
Amplitude & Phase plot of K *  
Particle Manipulation using DEP &
twDEP
Figure When a particle is suspended in an alternating electric field which contains either a
magnitude or phase gradient, a force is induced on the particle which acts
either in the direction
of the gradient or opposes it, according to whether or not the particle is more or less Polari able
than the medium in which it is suspended.
Here, a particle experiences force due to (a) a non-uniform electric field (magnitude gradient); (b)
a traveling electric field (phase gradient
Fabrication
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Electrode (Cr)
Micro-channel (PDMS)
Bonding (O2 plasma)
Ref: http://www.ndl.org.tw/old/ndlcomm/P12_1/3.pdf
Experiment Results

(1)
motion of whole blood cells being pumped by twDEP with
phasing between electrodes
Experiment Results

(2)
Effect of electrode gap and voltage on average cell velocity
Fdrag α Velocity
FDEP α (Voltage)*2
Experiment Results

(3)
Bi-directional manipulation
Fig. Computational result of twDEP force vectors
THE END
Thanks for Your Attention!