Download MDH Flow Chart for Rule-out and Referral of Bacillus anthracis

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MDH Flow Chart for Rule-out and
Referral of Bacillus anthracis
Public Health Laboratory - Emergency Preparedness and Response Unit
601 Robert St. N. PO Box 64899, St. Paul, MN 55164
651-201-5200
http://www.health.state.mn.us/divs/phl/labep/index.html
Colony Morphology on SBA
•
•
•
Large rough “ground glass” colony
Irregular shaped edges, comma like projections
“Sticky”—adheres to the agar
•
•
Large gram positive bacilli
1 -1.5 x 3 – 5 µm
Gram Stain Morphology
Large gram positive rod
Bacteria not a large gram
positive rod
Hemolysis
Hemolytic
Not B. anthracis
Not B. anthracis
Non-hemolytic
Catalase
Positive
Negative
Not B. anthracis
Motility
Negative
Positive
Not B. anthracis
REFER ISOLATE:
Cannot Rule Out B. anthracis
Call MDH-PHL at 651-201-5073
after hours number:
877-676-5414