Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Genetic diversity of microbial eukaryotes in anoxic sediment around
Document related concepts
Transcript
Diversity of eukaryotic microbes detected by culture-independent molecular analysis in sediments around fumaroles in Kagoshima Bay, Japan Kiyotaka Takishita1, Hiroshi Miyake1,2, Masaru Kawato1 and Tadashi Maruyama1 1. Res. Prog. Mar. Biol. Ecol., Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan E-mail address: [email protected] 2. Enoshima Aquarium, 2-19-1 Katase-Kaigan, Fujisawa, Kangnagawa, 251-0035, Japan While prokaryotic diversity has been studied in various environments, relatively little is known about the diversity of eukaryotic microbes (protist). To study their diversity in anaerobic environments using culture-independent molecular methods, we directly extracted DNA from anoxic sediment collected near active fumaroles on a submarine caldera floor (in Kagoshima Bay, Japan) at a depth of 200 m, and constructed genetic libraries of PCR-amplified eukaryotic small-subunit (SSU) rDNA…(The rest is omitted.)
