Download Powerpoint - Blood Journal

Reduced stem cell mobilization in mice receiving antibiotic
modulation of the intestinal flora: involvement of
endotoxins as cofactors in mobilization
by Gerjo A. Velders, Ronald van Os, Henny Hagoort, Perry Verzaal, Henri F. L.
Guiot, Ivan J. D. Lindley, Roel Willemze, Ghislain Opdenakker, and Willem E. Fibbe
Blood
Volume 103(1):340-346
January 1, 2004
©2004 by American Society of Hematology
The cytokine-induced mobilization of progenitor cells is decreased by treatment with
ciprofloxacin and polymyxin B. Mice were treated with 100 mg/L ciprofloxacin, 70 mg/L
polymyxin B, and 20 mg/L saccharose added to their drinking water for 1 week.
Gerjo A. Velders et al. Blood 2004;103:340-346
©2004 by American Society of Hematology
Treatment with oral polymyxin B reduces cytokine-induced stem cell mobilization, while
treatment with ciprofloxacin has no effect on IL-8– or G-CSF–induced mobilization.
Gerjo A. Velders et al. Blood 2004;103:340-346
©2004 by American Society of Hematology
IL-8–induced mobilization is significantly reduced after treatment with metronidazol.
Gerjo A. Velders et al. Blood 2004;103:340-346
©2004 by American Society of Hematology
In germ-free mice the IL-8–induced mobilization is significantly reduced.
Gerjo A. Velders et al. Blood 2004;103:340-346
©2004 by American Society of Hematology
The IL-8–induced mobilization in decontaminated mice is restored after oral administration of
endotoxins.
Gerjo A. Velders et al. Blood 2004;103:340-346
©2004 by American Society of Hematology
The numbers of CAFCs in peripheral blood are decreased after pretreatment with ciprofloxacin
and polymyxin B. Balb/c mice were treated with 100 mg/L ciprofloxacin, 70 mg/L polymyxin B,
and 20 mg/L saccharose added to their drinking water.
Gerjo A. Velders et al. Blood 2004;103:340-346
©2004 by American Society of Hematology