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Single-Cell Analysis of the t(14;18)(q32;q21) Chromosomal Translocation in Hodgkin's Disease Demonstrates the Absence of This Translocation in Neoplastic Hodgkin and Reed-Sternberg Cells by Sylvia Gravel, Georges Delsol, and Talal Al Saati Blood Volume 91(8):2866-2874 April 15, 1998 ©1998 by American Society of Hematology Single-cell isolation of one HRS cell from a frozen section of a lymph node involved by HD and immunostained with CD30/HSR4 MoAb. Sylvia Gravel et al. Blood 1998;91:2866-2874 ©1998 by American Society of Hematology Single-cell isolation of one OCI LY8 cell from a frozen section of OCI LY8 tumor generated into nude mice and immunostained with CD20/L26 MoAb. Sylvia Gravel et al. Blood 1998;91:2866-2874 ©1998 by American Society of Hematology Bcl-2 onco-protein expression in HRS in a case of nodular sclerosis HD as detected by anti–bcl2 MoAb on paraffin section. Sylvia Gravel et al. Blood 1998;91:2866-2874 ©1998 by American Society of Hematology Multiple PCR analysis of bcl-2/JH junction region using primers specific for the major translocation breakpoint region (MBR). Sylvia Gravel et al. Blood 1998;91:2866-2874 ©1998 by American Society of Hematology Multiple PCR analysis of the t(14;18)-MBR translocation of total DNA samples extracted from 3 reactive lymph nodes (RLN; cases a, b, and c). Sylvia Gravel et al. Blood 1998;91:2866-2874 ©1998 by American Society of Hematology Single-cell PCR analysis of the housekeeping genec-raf-1 (A) and the bcl-2/JH rearrangement (B) by seminested PCR analysis of cells isolated from frozen sections of OCI LY8 (lanes O: 1 through 3) and HSB-2 (lanes H: 1 through 3) tumor cells and of small lym... Sylvia Gravel et al. Blood 1998;91:2866-2874 ©1998 by American Society of Hematology Single-cell PCR analysis of the housekeeping genec-raf-1 and the bcl-2/JH rearrangement by seminested PCR analysis of cells isolated from frozen sections of a case of t(14;18)/MBRpositive follicular lymphoma (FL). Sylvia Gravel et al. Blood 1998;91:2866-2874 ©1998 by American Society of Hematology Single-cell PCR analysis of the t(14;18)-MBR translocation by seminested PCR analysis of isolated OCI LY8 tumor cells (lane O: 1 through 3), HRS cells from a lymph node involved by HD (lanes HD: 1 through 10), small lymphocytes from the same lymph node (lan... Sylvia Gravel et al. Blood 1998;91:2866-2874 ©1998 by American Society of Hematology Single-cell PCR analysis of the housekeeping genec-raf-1 by seminested PCR analysis of HRS cells from lymph node involved by HD (lanes HD: 1 through 10). Sylvia Gravel et al. Blood 1998;91:2866-2874 ©1998 by American Society of Hematology
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