Download Title: Supplementary Table 1 Caption: Cut

Title: Supplementary Table 1
Caption: Cut-points for protein markers used in different analyses. Cut-points selected for pair-wise comparisons were chosen to
reduce the total number of comparisons that would be done. Cut-points for the discovery/confirmation analyses (see
Supplementary Methods) were optimized based on enrichment of responders versus non-responders and reasonable population
size.
Protein marker (range)
Single-marker analyses
Pair-wise
Cross-validation
comparisons
model
PSMA5 % nuclear staining (0–100)
0–20, 30–50, 60–70, 80–90
≤20 vs. >20
≤20 vs. >20
PSMA5 % positive cytoplasmic signal (0–100)
0–20, 30–50, 60–70, 80–90
≤90 vs. >90
≤90 vs. >90
≤2+, 3+
≤2+ vs. >2
≤2+ vs. >2
CD68-positive (overall) (0–100)
0–25, 26–50, 51–75, >75
≤50 vs. >50
≤50 vs. >50
CD68-positive (follicular) (0–100)
0–25, 26–50, 51–75, >75
≤50 vs. >50
≤50 vs. >50
CD68-positive (peri-follicular) (0–100)
0–25, 26–50, 51–75, >75
≤50 vs. >50
≤50 vs. >50
p27 % nuclei positive (0–100)
0–20, 30–50, 60–70, 80–
≤70 vs. >70
≤70 vs. >70
≤1+ vs. >1
≤2+ vs. >2
PSMA5 cytoplasmic signal intensity (0–3+)
100
p27 signal intensity (0–3+)
≤1+, ≥2+
NF-κB p65 % nuclear staining (0–100)
0, ≤5, >5
0 vs. >0
0 vs. >0
NF-κB p65 % positive cytoplasmic signal (0–
≤90, >90
≤90 vs. >90
≤90 vs. >90
≤1+, ≥2+
≤1+ vs. >1
≤2+ vs. >2
100)
NF-κB p65 cytoplasmic signal intensity (0–3+)