Download Table S1.

Gene
Product
SecY
Amino
acid
37
64
65
67
68
69
71
73
90
188
191
274
277
278
282
286
407
408
SecE
SecG
SecA
411
105
108
116
117
120
25
26
50
57
65
111
134
Mutant
alleles
prlA8911
prlA300
prlA8914
prlA3
prlA666
prlA726
prlA799
prlA8913
prlA9
prlA205
prlA302
prlA306
prlA304
prlA6
prlA200
prlA1
prlA7
prlA202
prlA208
prlA303
prlA401
prlA4
prlA7, 11
and 301
prlA4 and
6
prlA11
prlG2
prlG1
prlG8
prlG8
prlG3
prlH2
prlH1
prlH3
prlH4
prlH6
prlH5
prlD4
prlD22
Mutation
details
Ser → Phe
Phe → Cys
Asn → Tyr
Phe → Cys
Phe → Ser
Ser → Pro
Ser → Leu
Ser → Phe
Gly → Asp
Gly → Cys
Ala → Asp
ΔSer
Ile → Asn
Ser → Leua
Ile → Ser
Val → Gly
Ala → Glub
Ile → Ser
Ile → Asn
Ile → Thr
Ser → Arg
Phe → Tyrc
Leu → Argb,d
M. endobia
status
38Ser
65Phe
66Asn
68Phe
E. coli
codon
TCT
TTT
AAC
TTC
M. endobia
codon
TCG
TTT
AAT
TTC
69Ser
TCT
TCT
70Gly
GGT
GGT
72Ala
74Ser
91Ile
189Ser
192Ile
276Val
279Thr
280Ile
GCT
AGC
ATC
TCA
ATC
GTA
GCA
ATC
GCA
AGC
ATC
TCC
ATT
GTT
ACA
ATT
284Ser
288Phe
409Leu
AGT
TTC
CTT
AGC
TTT
TTG
Ile → Asna,c
410Ile
ATC
ATA
Val → Glyd
Ser → Pro
Leu → Arg
ΔVale
ΔArge
Ser → Phe
Gly → Asp
Lys → Glu
Asn → Tyr
Asn → Ile
Ala → Glu
Ile → Thr
Thr → Asn
Tyr → Cys
413Val
106Ser
109Leu
117Val
118Arg
121Ser
25Gly
26Lys
50Thr
GTC
TCA
CTG
GTT
CGC
TCC
GGT
AAA
AAC
GTG
TCA
CTG
GTT
CGT
TCA
GGT
AAA
ACT
57Ala
65Val
111Thr
134Tyr
GCG
ATC
ACC
TAC
GCA
GTG
ACT
TAC
1
148
373
484
488
507
645
1
2
3
azi-17
prlD21
and 23
prlD21
prlD5
prlD43
prlD2 and
3
prlD20,
24, 26-28
and 31
azi-4
Tyr → Asn
Tyr → Serf
Glu → Lysf
Ala → Val
His → Gln
Ala → Val
148Asp
373Ala
484His
488Ala
GAA
GCT
CAC
GCG
GAT
GCG
CAC
GCA
Ala → Val
507Ala
GGC
GGC
Leu → Gln
645Leu
CTG
CTA
a
Mutations in double mutant prlA6; bMutations in double mutant prlA7; cMutations in
double mutant prlA4; dMutations in double mutant prlA11; eMutations in prlG8;
f
Mutations in double mutant prlD21.
2