Download 8. Preparation of blood smear using chicken`s blood

Practical work.........21
8. Preparation of blood smear using chicken’s blood
Procedure
1.
Collect some blood of a freshly killed chicken
with a bottle containing some anticoagulant
(e.g. sodium citrate).
2.
Place a drop of blood onto one end of a slide.
3.
Make a blood smear as shown in the figures
below:
4.
5.
6.
7.
8.
Leave the blood smear for three to four minutes
Taking
human
until it is dry.
blood and cell samples in
Add one or two drops of Leishman’s stain and
school laboratories carries a
one to two drops of distilled water on the slide.
risk
Leave the slide for five minutes and wash off
blood-borne diseases such as
the surplus stain under a gently-running tap.
AIDS
Shake off the surplus water and leave the slide
Deficiency Syndrome) and
to dry.
Viral
Examine the slide under the microscope.
Experiments like mounting of
of
transmitting
(Acquired
Hepatitis
human
cheek
Immune
B.
cells,
Preparation of Reagent
preparation of human blood
1.
Anticoagulant
smear and investigation of the
0.106M of sodium citrate solution can be
osmotic tolerance of human
prepared by dissolving 3.13 g of the dihydrate
red blood cells are at such
salt in 100 cm3 of water. One volume of the
risk. Such practices should be
anticoagulant is recommended for 9 volumes of
discontinued
blood.
laboratories.
in
school