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The University of Michigan Transgenic Animal Model Core Sally A. Camper, Ph.D. Linda C. Samuelson, Ph.D. Thomas L. Saunders, Ph.D. 2570 MSRB II, SPC 5674 1150 W. Medical Center Drive Ann Arbor, MI 48109-0674 Voice: (734) 647-2910 FAX: (734) 936-2622 http://www.med.umich.edu/tamc/ Internal Use Only Date Received Log Number Logged By BAC recombineering services include DNA purification and verification by pulsed field gel restriction mapping. Name of BAC: Gene of Interest: Species: Genome Coordinates and Genome Build: Contact Michael Zeidler ([email protected] or 763-6209) to initiate a BAC recombineering project. If available, email the sequence of the wild type BAC and the desired sequence of the genetically modified BAC to Mr. Zeidler. The Core will review the design and submit a final sequence for approval. Available Services: 1. Knockins. Available cassettes include eGFP dsRedMTS Flagtag epitope human placental alkaline phosphatase cDNA for gene of interest point mutations iCre-ERT2 knockin and removal of loxP site in BAC cloning vector iCre knockin and removal of loxP site in BAC cloning vector Inquiries about other expression cassettes are welcome. 2.Remove of loxP site in BAC cloning vectors (pBACe3.6, pBeloBAC11) 3.Subclone DNA fragment from BAC by gap repair 4. Construction of conditional or null gene targeting vector. Contact Person Phone Number email address: ShortCode Number for Core Recharge P.I. Name Funding Source Grant Title Billing Department Authorized Signature