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Biology 263 Cell Biology Dr. Joel B. Piperberg December 17, 2009 Final Exam Name _________________________________ Page #1 I. Multiple Choice. Fill in the best answer on the answer sheet. 1. What is the name given to carbohydrates bound to serine or threonine residues on a secretory protein? a. N-linked carbohydrates b. O-linked carbohydrates c. proteoglycans d. glycoproteins e. a and b 2. What controls the sequence of carbohydrates on oligosaccharides attached to secretory proteins? a. whatever carbohydrates are close to the proteins b. the sequence of galactose oxidases in the RER and SER c. the arrangement and sequence of glycosyltransferases in the RER, SER and Golgi apparatus d. the sequence of nucleotides in the nucleotide sugars e. b and c 3. ________ attach cells to _________. a. Hemidesmosomes, a noncellular surface c. Maculae adherens, neighboring cells e. a, b and c b. Zonulae adherens, neighboring cells d. Zonulae adherens, a noncellular surface 4. What is the name of the cytoskeletal element associated with maculae adherens? a. keratin b. microfilaments c. tubulin d. a and e e. tonofilaments 5. You mix secretory and domestic protein mRNA, ribosomes, naked RER vesicles that have been stripped of their ribosomes and everything else you need to synthesize proteins in a test tube and let them incubate for 30 minutes. After the incubation, what do you find in the test tube? a. domestic proteins floating free in the buffer in the test tube b. secretory proteins inside RER vesicles with ribosomes bound to their inner surface c. secretory proteins inside RER vesicles with ribosomes bound to their outer surface d. domestic proteins in the RER vesicles with ribosomes bound to their inner surface e. a and c 6. Which cell junction below may attach epithelial cells to each other and are built to withstand a great deal of force in holding cells together? a. zonulae occludens b. maculae adherens c. zonulae adherens d. belt desmosomes e. b, c and d 7. What changes can be made in a secretory protein after it has already entered the RER? a. addition of new amino acids to the signal sequence d. b and c b. addition of a segment of the amino acids to the interior of the secretory protein e. a, b and c c. addition of sugars to the carbohydrates donated to the protein by dolichol phosphate 8. Which cell adhesion molecules that bind to specific carbohydrates on the cell surface are named after a group of plant molecules that are known for their ability to bind specific carbohydrates? a. selectins b. lectins c. IgSFs d. integrins e. cadherins 9. What cell junction(s) is (are) responsible for preventing materials from getting past an epithelium like the urinary bladder epithelium? It does so by preventing these materials from moving between epithelial cells. a. gap junctions b. gapulae adherens c. maculae adherens d. tight junctions e. c and d 10. Which enzyme would be most likely to be located in a peroxisome? a. RNase b. amino acid oxidase c. b and d d. catalase e. acid phosphatase 11. In which organelle would most secretory proteins receive their first glycosylation? a. RER b. secretory vesicles c. Golgi apparatus d. SER e. a and d Biology 263 Cell Biology Dr. Joel B. Piperberg December 17, 2009 Final Exam Name _________________________________ Page #2 12. A pulse-chase experiment is carried out by exposing rat parotid cells to radioactive glycerol for a brief period of time followed by increasing chase periods in the presence of unlabeled glycerol precursors? What is the most important revelation of this technique? a. the time it takes to synthesize a single membrane lipid b. the pathway membrane lipids follow through the cell on their way to the membrane c. the time it takes for a secretory protein to be secreted d. the pathway membrane proteins follow through the cell on their way to the membrane e. c and d 13. Which organelle is most prominent in the photosynthetic tissues of plants? a. peroxisomes b. lysosomes c. glyoxysomes d. contractile ring e. centrioles 14. You mix radiolabeled mouse liver cells with a number of different cell types, each grown to cover separate Petri dishes. The radiolabeled cells are most likely to adhere to which cell type below? a. mouse liver b. rat liver c. a and e d. a and b e. mouse retina 15. What may account for differences in the appearance of RER and SER? a. the presence of ribosomes on the RER d. the distribution of ribosome docking proteins (translocons) b. the relative amounts of certain enzymes e. a, b and d c. the relative amounts of certain nucleic acids 16. The mRNA for a well-known secretory protein is isolated and placed in a test tube in the presence of all the substances required for in vitro protein synthesis. The sequence of the protein produced in vitro is then compared to the sequence of the purified secretory protein from the cell. The amino acid sequences of the two proteins are not the same. What is the explanation? a. A short chain of mostly hydrophobic amino acids was removed from the C-terminal end of the polypeptide in the test tube before it was analyzed. b. A short chain of mostly hydrophilic amino acids was chopped off the N-terminal end of the protein purified from the secretory cell. c. The mRNA was cleaved after being translated in the test tube, d. A largely hydrophobic segment at the N-terminal end of the polypeptide is cut off of a protein synthesized in vitro (in the test tube) and degraded by the signal peptidase. e. none of the above 17. Dolichol phosphate ____________. a. is a phosphatidyl protein b. donates a block of carbohydrates to proteins destined for the cell membrane as they project through the RER membrane c. is a membrane phospholipid d. and its attached carbohydrates flip from the membrane leaflet facing the RER lumen to the cytoplasmic leaflet e. b and c 18. Which enzyme would be most likely to be found in a lysosome? a. b and d b. acid phosphatase c. catalase d. hexosaminidase 19. Which organelle is observable only in an animal cell? a. mitochondria b. vacuole c. glyoxysome d. peroxisome e. amino acid oxidase e. none of the above 20. Storage diseases like Hurler's syndrome and Tay-Sachs disease may result from ______ enzymes. Biology 263 Dr. Joel B. Piperberg Final Exam Page #3 a. nonfunctional peroxisomal b. missing mitochondrial Cell Biology December 17, 2009 Name _________________________________ c. nonfunctional lysosomal d. overabundant lysosomal e. c and d 21. Which name below would be the name of an enzyme in the RER, SER or Golgi apparatus that is responsible for adding sugars to growing oligosaccharide chains attached to the proteins passing through them? a. glucosyltransferase c. peptidylsynthase e. glycosylsynthetase b. a and d d. glycosyltransferase 22. Which of the following molecules has been reduced by binding to high-energy electrons? a. ATP b. FADre c. FADox d. NADre e. b and d 23. You are studying some photosynthetic bacteria found in the sulfur pools of Yellowstone Park. They contain only one photosystem and yet seem to be able to carry out both cyclic and non-cyclic photophosphorylation. The sulfur in the pools is found in various forms including hydrogen sulfide (H2S), an electron-rich compound. How could the bacteria carry on photosynthesis with just one photosystem? a. none of the other answers b. They acquire electrons from water to replace those stored in NADPox. c. They acquire electrons from water to replace those stored in NADre. d. They acquire electrons from hydrogen sulfide to replace those stored in NADPox. e. They make ATP whether they are carrying on respiration or non-cyclic photophosphorylation. 24. In what part of the cell can lysosomes form? a. b, c and d c. GERL b. rough endoplasmic reticulum d. in the Golgi apparatus e. c and d 25. Fibronectin is likely to have binding sites for which of the following molecules? a. cell surface proteins b. collagen c. tubulin d. GAGs e. a, b and d 26. Which word or words below is associated with a receptor tyrosine kinase? a. insulin b. serine c. cAMP d. transautophosphorylation e. a and d 27. What second messenger appears to be responsible for relieving the pain of angina? a. carbon monoxide b. nitrous oxide c. monoamine oxidase d. NO e. b and d 28. The cell coat is composed of ____________. a. carbohydrates attached by covalent bonds to membrane proteins b. carbohydrates attached noncovalently to transmembrane proteins c. the glycocalyx and the fuzzy layer d. a and b e. a, b and c 29. The function of the glycocalyx is __________. a. to generate friction between neighboring cells d. b, c and e b. to mediate interactions between cells e. to act as a molecular sieve c. to prevent large molecules from getting to the cell membrane 30. Radiolabeled monosaccharides are given to growing secretory cells. Over which of the following structures would silver grains first appear? a. rough ER b. Golgi apparatus c. secretory vesicles d. a and e e. smooth ER 31. Patients with I-cell disease __________ Biology 263 Cell Biology Dr. Joel B. Piperberg December 17, 2009 Final Exam Name _________________________________ Page #4 a. may make excess receptors for mannose-6-phosphate in carbohydrates attached to lysosomal enzymes b. may be unable to add phosphate groups to mannose residues in the carbohydrates of lysosomal enzymes c. synthesize peroxisomal enzymes without properly marking them as such d. are unable to place lysosomal enzymes effectively within lysosomes e. b and d 32. What characteristics of the signal peptide allow it to penetrate the RER membrane easily? a. It is 15 - 30 amino acids long. d. It is mostly made up of hydrophilic amino acids. b. Its sequence is recognized by the GERL. e. c and d c. Its central 9 - 12 amino acids are primarily hydrophobic. 33. Which of the following is a function of the rough endoplasmic reticulum? a. It adds sugars to carbohydrate chains on presumptive membrane proteins. b. It can excise amino acid segments from proteins. c. It synthesizes domestic proteins. d. It synthesizes secretory proteins. e. a and d 34. According to chemiosmosis, the energy stored in ATP by mitochondrial electron transport is generated when __________. a. ATP synthetase produces NADPre in addition to ATP b. electron transport drives a transmembrane H+ ion pump that establishes a steep H+ ion gradient c. electrons are transported hyperkinetically d. energy is stored in an electrochemical gradient between the thylakoid disk lumen and the stroma e. b and d 35. You are conducting an experiment in photosynthesis. You have radioactively labeled the oxygen in a compound and have observed that this oxygen is soon seen in the carbohydrates of the plant. What compound was labeled? a. water b. carbon monoxide c. starch d. carbon dioxide e. glucose 36. Some of the molecules of the electron transport chain in chloroplasts accept electrons while others accept electrons and hydrogen ions. This is significant because __________. a. it explains how these molecules can transport protons for the purpose of setting up a proton gradient b. it explains how the energy that helps to make ATP in the chloroplasts is generated c. a and b d. it explains how electrons are added to NADPre e. it explains why Ralph Nader is such a jerk. (Come to think of it - nothing explains that) 37. The importance of the Krebs (citric acid) Cycle in cell metabolism is that it ___________. a. makes important biochemical intermediates c. makes NADox e. a, b and d b. makes FADre d. makes ATP 38. Fermentation permits continuous glycolysis in plants because ___________. a. it serves to recycle NADre, which is needed for glycolysis d. lactic acid is produced, recycling NADox b. it serves to recycle NADox, which is needed for glycolysis e. b and d c. there is a net increase in the production of NADPox 39. Which statement below about noncyclic photophosphorylation is true? Biology 263 Cell Biology Dr. Joel B. Piperberg December 17, 2009 Final Exam Name _________________________________ Page #5 a. ATP is produced from the electron derived from the Photosystem II reaction center. b. O2 is produced when H2O is split c. a and b d. The electron from the Photosystem I reaction center ends up back in Photosystem I. e. Electrons from Photosystem I drive the production of ATP. 40. A scientist isolates two specific mRNAs. One codes for the enzyme tigerase, a really good golfer enzyme that strays; the other codes for the enzyme beckase, a blowhard enzyme that fake cries on TV and knows a lot less than it thinks. Both mRNAs are placed in a test tube with RER vesicles stripped of their ribosomes, free ribosomes and precursors for protein synthesis. When the protein synthesis reaction is complete, beckase is found in the liquid portion of the test tube outside the vesicles; tigerase is found inside the vesicles. What can one conclude about the two proteins? a. Beckase is a secretory protein; tigerase is a domestic protein. b. Beckase is a domestic protein; tigerase is a secretory protein. c. Both proteins are secretory. d. Both proteins are meant for domestic use. e. Tigerase diffuses into the vesicle after synthesis; beckase is an intrinsic membrane protein. 41. Which of the following sequences is most likely to be part of a signal peptide of a secretory protein. Refer to the diagram of amino acid R groups on the last page of the multiple choice section for help. a. — gly - asp - met - glu - asp - gly - lys - arg - met — b. — cys - arg - glu - lys - lys - arg - gly - met - asp — c. — arg - leu - asp - lys - phe - glu - arg - pro - leu — d. — lys - ala - met - gly - phe - gly - leu - pro - met e. — leu - arg - lys - asp - glu - met - gly - asp - arg 42. What molecule binds to electrons that drop off of the end of the mitochondrial electron transport chain forming another common compound? a. NADPre b. O2 c. NADre d. H2O e. CO2 43. Which of the following occurs when ATP is produced from the joining of an ADP and a phosphate group? a. Energy is released. c. b and d e. an hydrolysis reaction b. Water is released. d. a condensation-dehydration reaction 44. Which event(s) occur(s) during the glucose mobilization (pump priming) stage of glycolysis? a. d and e b. Two molecules of acetyl CoA transfer electrons to a 4-carbon molecule c. Two molecules of ATP are used up. d. A 6-carbon sugar is produced with 2 phosphate groups attached to one end e. Glucose is changed into a compound that is readily cleaved into three 2-carbon phosphorylated units. 45. Which statement about glycolysis is true? a. It uses molecular oxygen. d. b and e b. It occurs in plant cell cytoplasm e. Glycolysis results in the gross production of 4 ATPs c. Every living creature on Earth and viruses can carry out glycolysis. 46. What molecule carries 2 carbons at one point during aerobic respiration? a. b and d b. citric acid c. acetyl CoA d. succinyl CoA e. NADre 47. What would happen in a cell if the electron transport chain were blocked? a. NADre levels would decrease. c. There would be a net decrease in oxygen. e. a, b and d Biology 263 Dr. Joel B. Piperberg Final Exam Page #6 b. NADox levels would increase. Cell Biology December 17, 2009 Name _________________________________ d. FADre levels would increase. 48. Given that glycolysis, Krebs cycle and electron transport are regulated by allosteric enzymes that control the reactions, what do you think happens to phosphofructokinase, one of the first enzymes in the glycolytic pathway, when ATP levels are very low? a. ATP is not found at the enzyme’s allosteric site and it works more quickly. d. Pyruvate production rises. b. It is inhibited and begins to work more slowly. e. a and d c. It denatures. 49. The lollipop structures are ___________. a. b, c and d c. ATPase molecules b. found on thylakoid membranes d. found on the cristae membrane e. b and d 50. What is the net gain in ATPs when 32 molecules of glucose are processed aerobically in a cell with high energy requirements? a. 1156 b. 32 c. 1216 d. 36 e. 38 51. How many carbons from 45 original glucose molecules enter the Krebs cycle when a cell operates aerobically? a. 180 b. 270 c. 4 d. 6 e. none 52. How many ATPs are made from 72 original glucose molecules when a cell is involved in fermentation? a. 144 b. 1368 c. none d. 1296 e. 72 53. An animal cell is injected with a low molecular weight fluorescent molecule called fluorescein. After a short period of time, adjacent cells are seen to be fluorescing just like the originally injected cell. The explanation is that the cells ________. a. are connected by plasmodesmata d. were damaged by the injection procedure b. are connected by gap junctions e. are connected by hemidesmosomes c. are joined by gap junctions & plasmodesmata 54. Which metabolic pathway probably evolved first? a. Krebs cycle c. cyclic photophosphorylation b. noncyclic photophosphorylation d. glycolysis e. fermentation 55. How would one determine the time it takes for the proteins destined for secretion to be synthesized, processed and finally secreted and the pathway the protein follows at the same time? a. c and d c. pulse-chase experiment e. b, c and d b. labeling with radioactive sugars d. autoradiography 56. You have just found a dead deep-sea creature in your fishing nets. It appears to be a new species. During the post-mortem attempt to classify the organism, a jelly-like, extracellular mass is found in the abdomen. It is found to contain protein and carbohydrate and lots of water. What kind of molecule would you expect to find in the jelly-like mass? a. proteoglycan aggregates b. GAGs c. proteomucoids d. mucopolysaccharides e. a, b and d 57. In which cell junction do the membranes have the same distance separating them as in regions of the membrane where there are no cell junctions? a. spot desmosomes b. maculae adherens c. tight junctions d. gap junctions e. a and b 58. You isolate the gene for a secretory protein and remove the nucleotides that code for the first 30 amino acids on the protein's N-terminal end. You are then able to add this nucleotide sequence to the beginning of the gene for a domestic protein. You are able to replace the normal gene for this domestic Biology 263 Cell Biology Dr. Joel B. Piperberg December 17, 2009 Final Exam Name _________________________________ Page #7 protein in a rat cell with the altered form of the gene you have produced. When the rat cell and its descendents start to make this protein where can it be found? a. in the RER lumen c. in the Golgi complex e. a and c b. in the peroxisomes d. in the cytoplasm 59. __________ allow molecules with low molecular weights to pass between cells via a direct connection between their cytoplasms. The purpose of this connection is to allow communication between the cells so that they can _________. a. Plasmodesmata, act in concert c. Gap junctions, swell e. a and d b. Plasmodesmata, disaggregate d. Gap junctions, communicate with each other 60. A red sponge is dissociated mechanically and a yellow sponge is dissociated chemically by placing it in Ca+2 - Mg+2 - free sea-water. Both cell suspensions are washed in natural sea-water a number of times and placed together in natural sea-water at 4°C. Washes were performed by centrifuging the dissociated sponge cells and removing the supernatant. If the supernatant from the first centrifugation (i. e., the solution surrounding the cells during dissociation) of the yellow sponge is added to the above mixture of red and yellow sponges, what happens? a. Neither sponge reassociates. d. Both sponges reassociate into one mass b. The red sponge reassociates; the yellow sponge does not. e. Both sponges reassociate into separate masses. c. The yellow sponge reassociates; the red sponge does not 61. What happens if the mixture of dissociated red and yellow sponges mentioned above is cultured at 4°C with the addition of supernatant from the initial centrifugation of the red sponge? a. Both sponges eventually reassociate into one mass. d. The red sponge reassociates; the yellow does not. b. Neither sponge reassociates. e. Both sponges eventually reassociate independently. c. The yellow sponge reassociates; the red does not. 62. Three tissues (A, B and C) from a vertebrate embryo are dissociated and then allowed to reassociate in pairs. If in these aggregates, Tissue B ends up internal to Tissue C and Tissue B ends up external to Tissue A, then: a. Tissue C will be external to Tissue A c. Tissue A will be external to Tissue C e. a and b b. Tissue A will be internal to Tissue C. d. Tissue A will be internal to Tissue D. 63. What kind of disease would be likely to be caused by a defect in hemidesmosomes? a. a disease in which cells die indiscriminately b. a disease in which blisters form in the skin c. a disease in which gashes form between skin cells d. a disease in which cancerous lesions form frequently e. a disease in which dark patches form in the skin 64. A disease in which an epithelial layer designed to contain liquid secretions leaks somewhat causing inflammation in its vicinity is most likely to indicate defects in which cellular structures? a. mitochondria b. hemidesmosomes c. zonula occludens d. spot junctions e. plasmodesmata 65. What organelle is responsible for converting lipids directly to carbohydrates? a. lysosome b. glyoxysome c. chloroplast d. peroxisome e. mesosome 66. Starting with the apical surface of cells that secrete proteins into a duct at that surface, in what order would you encounter cell junctions if you could travel between the cells from the apical surface toward the basal surface of the cells? a. belt desmosome, tight junction, spot desmosome d. tight junction, belt desmosome, spot desmosome Biology 263 Dr. Joel B. Piperberg Final Exam Page #8 Cell Biology December 17, 2009 Name _________________________________ b. tight junction, spot desmosome, belt desmosome e. spot desmosome, gap junction, belt desmosome c. spot desmosomes, belt desmosomes, tight junctions 67. Which cell junction would prevent proteins embedded in the cell membrane and located on the lateral or basal surface of the cell from moving to the apical surface of those same cells? a. zonula adherehns b. belt desmosomes c. b and d d. tight junctions e. a and d 68. A(n) _______ located in the _______ is a modified lysosome. a. acrosome, unfertilized egg c. acrasin, peroxisome e. acrosome, head of a sperm b. mesosome, head of the sperm d. acrasin, lysosome 69. The first sugars added to dolichol phosphate are added in what location? a. in the RER lumen b. in the SER lumen c. in the Golgi d. in the cytoplasm e. on the ribosome 70. Which of the following happens after the SRP leaves the ribosome bound to the RER? a. Protein synthesis begins again. d. The protein being synthesized enters the cytoplasm. b. Protein synthesis is temporarily suspended. e. a and c c. The protein being synthesized enters the RER lumen. 71. Which of the molecules below is an example of the type of molecule that glycosyltransferases require to complete their reaction of adding sugars to growing oligosaccharide chains? a. nucleotide sugars b. UDP-glucose c. ATP d. NADre e. a and b 72. Which of the following proteins would be likely to be synthesized on free cytoplasmic ribosomes? a. integrins b. glycolytic enzymes c. steroid receptors d. b and c e. insulin receptors 73. In order for a protein to end up in the cell membrane, which of the following things would it need to have as part of its initial primary structure? a. a signal peptide b. a signal peptidase c. a sequence that opens the translocon d. a 5'-end e. a and c 74. Which of the cell adhesion molecules below always requires the presence of calcium? a. integrins b. sponge cell aggregation factors c. cadherins d. b and c e. IGSFs 75. Which of the following can be found attached to the outer leaflet of the RER? a. signal peptidase b. dolichol phosphate c. ribosomes d. an RNA enzyme e. b, c and d 76. What is the name of the process that is responsible for destroying the organelles of reticulocytes, the cells that mature into red blood cells? a. autophagy b. autolysis c. apoptosis d. extracellular digestion e. b and c 77. Which of the following activities is triggered by the release of calcium ions? a. secretion b. exocytosis c. muscle contraction d. activation of a fertilized egg e. all of the above 78. What process is responsible for the restructuring of bone during an organism’s life and the destruction of potential cancer cells when their abnormalities are detected? a. autophagy b. autolysis c. apoptosis d. extracellular digestion e. b and c 79. Strauss performed an experiment in 1964. He injected the enzyme horseradish peroxidase into his experimental organism. Some cells in the organism took up the horseradish peroxidase by endocytosis. Eventually, the endosomes containing horseradish peroxidase met up with lysosomes and merged with them. What color were the vesicles containing the horseradish peroxidase after they were presented with a special substrate for the enzyme and before they fused with a lysosome? Biology 263 Dr. Joel B. Piperberg Final Exam Page #9 a. yellow b. blue Cell Biology December 17, 2009 Name _________________________________ c. red d. green e. purple 80. Integrins bind to a specific three amino acid sequence (designated RGD) in a number of proteins. A dish is coated with a protein containing the RGD sequence. Some cultured cells with integrins embedded in their membranes were added to the culture dish along with tripeptides with the RGD sequence. The cells do not bind to the dish. This is an example of _________. a. allosteric inhibition c. a and b e. receptor degradation b. noncompetitive inhibition d. competitive inhibition H CH2 CH2 CH2 N arginine C NH2 CH NH CH 3 CH 2 CH leucine CH O CH 2 aspartate CH2 SH cysteine C O HO CH OH tyrosine CH 3 CH valine CH alanine CH CH S CH 2 methionine O CH2 CH2 C NH2 glutamine 2 2 CH CH OH 2 serine C 2 OH O CH 2 CH 2 CH 2 CH 3 CH3 OH threonine C 2 NH lysine H H C CH 2 glutamate 3 CH3 H glycine 2 phenylalanine 3 CH 2 CH CH3 isoleucine CH 2 NH2 asparagine CH 2 CH3 CH2 C 2 2 histidine NH HN + C H CH2 HC N proline H CH C CH 2 tryptophan C HC N H III. Lecture Short Answer 1. You have fractionated a plant cell into a cytoplasmic portion, a nuclear portion, a fraction composed of the inner mitochondrial membranes, a fraction composed of the outer mitochondrial membranes, a fraction composed of thylakoid membranes, a fraction containing the chloroplast stroma and a fraction containing the mitochondrial matrix. Where would you expect to find the a. the enzymes responsible for glycolysis? b. ATP synthase? c. respiratory electron transport chain? d. photosystem II? e. Krebs cycle? (5 points) 2. What lysosomally controlled cell process is exemplified by: a. digestion of the membranes around an egg by a sperm cell b. digestion of a bacterium phagocytosed by an amoeba c. the resorption of the tadpole’s tail Biology 263 Dr. Joel B. Piperberg Final Exam Page #10 Cell Biology December 17, 2009 Name _________________________________ d. the breakdown of cellular organelles to buy time in the face of starvation (4 points) 3. What does SRP stand for? What are the two major components of the SRP? (3 points) 4. What pathway do secretory proteins follow on their way to the cell surface, starting with their synthesis? (4 points) 5. Where are peroxisomes assembled? What fact about its evolutionary history does the peroxisome’s site of production reveal? (2 points) 6. What is the name of the process that generates most of the energy that is used to produce ATP in the mitochondrion? It creates an electrochemical gradient. (1 point) 7. What is an IRS? (1 point) BONUS QUESTIONS 1. Describe the flow of electrons during noncyclic photophosphorylation from the place where they originate to where they eventually end up? What drives their movement? (3 points) 2. The following statements apply to photosynthesis, cellular respiration, both processes or neither process. Indicate which process is referred to in each statement by writing either P, C, PC or N in the blank before each statement. (14 points) _______ a. Produces oxygen as a product _______ b. ATP is used up at some point in the process _______ c. Part of the process occurs in the chloroplast _______ d. Generates phosphorylated lipids _______ e. Involves the splitting of a water molecule Biology 263 Cell Biology Dr. Joel B. Piperberg December 17, 2009 Final Exam Name _________________________________ Page #11 _______ f. Reduced coenzymes and other electron-carrying molecules are oxidized during the process _______ g. Carbon dioxide is a reactant in this process _______ h. During this process, a six-carbon molecule is formed from a two-carbon fragment and a four-carbon fragment _______ i. Some of the reactions involved occur in a milieu called the stroma _______ j. Uses chemiosmosis as the source of energy to make ATP _______ k. UTP is produced during the process _______ l. Produces NADPre at some point in the process. _______ m. Water is produced during this process _______ n. FADox is produced during the process 3. What do plants produce when they operate anaerobically? (2 points) 4. When animal cells operate anaerobically, what do they generally produce? (1 point) 5. Cyclic photophosphorylation normally results in the production of ___________. (1 point) 6. Name a molecule that is a relative of ATP that participates in photosynthesis. (1 point) 7. What is another name for autolysis? (1 point) 8. You are working with a gene in yeast cells. After cloning and sequencing it, you discover that there is a very similar gene in humans. If you take a normal version of the human gene and introduce it into a yeast cell that possesses a mutant version of its corresponding gene, what is likely to happen? (1 point) 9. Who was the President at the start of the Great Depression? (1 point) 10. Name two actors who have portrayed Vito Corleone. (2 points) 11. What is crossing over and why is it advantageous? (2 points) 12. An organism has a diploid number of 36. How many total chromosomes are in 12 of its sperm cells? (1 point) Biology 263 Cell Biology Dr. Joel B. Piperberg December 17, 2009 Final Exam Name _________________________________ Page #12 13. An organism has a haploid number of 48. How many total chromosomes are there in 14 kidney cells from this organism? (1 point) 14. Which phase of mitosis or meiosis is defined by the following descriptions? Specify the phase and whether you are talking about mitosis, meiosis I, or meiosis II. (6 points) a. A haploid number of chromosomes is lined up in the center of the cell; each chromosome consists of 2 chromatids. b. A diploid number of chromosomes splits at the centromere and begins moving to the opposite poles of the cell. c. A haploid number of chromosomes decondenses and becomes sequestered in the newly formed nuclei of 4 daughter cells; each chromosome consists of 1 chromatid. d. A haploid number of chromosomes condenses and each can be seen to consist of two chromatids; they are unpaired and the nuclear membrane begins to disintegrate. e. A diploid number of unpaired chromosomes lines up in the center of the cell. f. A haploid number of chromosomes separates into two daughter nuclei; each chromosome has 2 chromatids. 15. Who was the President who immediately preceded Abraham Lincoln? (1 point) 16. To what does an SH2 domain bind? (1 point) 17. Name an ECM glycoprotein that helps to direct the migration of cells during embryonic development. (1 point) 18. Why is the number of chromosomes cut in half during meiosis? (2 points) 19. What kind of molecule is most prominent in the vitreous humor of the eye? (1 point) 20. What is wrong with the following list of molecules? (1 point) Biology 263 Cell Biology Dr. Joel B. Piperberg December 17, 2009 Final Exam Name _________________________________ Page #13 UDP-glucose, UDP-galactose, CMP-sialic acid, CMP-glucuronic acid, UDP-sialic acid 21. What type of molecule(s) is (are) best described as large, highly hydrophilic molecules that easily participate in the formation of gels at low concentration? The molecule has a core of hyaluronic acid. (1 point) 22. What type of signaling is typified by multiple receptors influencing a single effector? (1 point) 23. Name one molecule that activates a receptor tyrosine kinase? (1 point) 24. What is the name of the membrane proteins that interact with elements of the extracellular matrix (ECM)? Their binding of the ECM could lead to changes in the cytoskeleton and gene expression (1 point) Biology 263 Dr. Joel B. Piperberg Final Exam Page #14 Cell Biology December 17, 2009 Name _________________________________ Merry Christmas, Happy Chanukah, Wonderful Kwanzaa and Happy New Year after you finish the survey below but not before. Course Survey (2 points) 1. The thing I liked most about this course was 2. The thing I liked least about this course was 3. If I could change one thing about this course, it would be 4. The lab I liked most was 5. The lab I liked least was 6. What topic that was not discussed would you most like to hear about? 7. General comments