Download Multivariate analysis of metal concentration in parental lines of

Multivariate analysis of metal concentration in parental lines of wheat
mapping populations
Laura Del Coco,a Barbara Laddomada,b Danilo Migoni,a Giovanni Mita,b Rosanna
Simeone,c Francesco Paolo Fanizzia
a
University of Salento, DiSTeBA, Campus Ecotekne, via Prov.le Lecce-Monteroni, 73100, Lecce, Italy,
b
Istituto di Scienze delle Produzioni Alimentari, CNR, Via Prov.le Monteroni, 73100 Lecce, Italy ,
c
Dipartimento di Scienze del suolo, della pianta e degli alimenti, Sezione Plant Breeding, Università di Bari
Via Amendola, 165/a, 70124, Bari, Italy
e-mail: [email protected]
Wheat is a staple cereal and an important source of minerals in human diets. Mineral
content in the wheat grain has a genetic basis, but the trait is also influenced by
environmental factors [1]. Many efforts have been devoted to increase the content of some
elements such as Fe, Zn, Mn and Cu, which have a physiological function in the organism.
In particular, mapping populations and genetic linkage maps have been suggested as a
useful tool in breeding programs [2]. The present work was undertaken to evaluate by
ICPOES the variation in macronutrients (Ca, Mg, K), micronutrients (Zn, Fe, Cu, Mn) and
toxic trace elements (Cd, Pb) in the whole grains of 29 wheat accessions and cultivars,
representative of old and modern wheat germplasm. The wheat genotypes were grown in
the experimental field of the University of
Bari at Valenzano (Bari, Italy) in 2013/14
durum wheat
wild wheat
(site A) and at Policoro (Matera) in 2014/15
common wheat
(site B), in a randomized complete block
design with three replications and plots [3].
Multivariate Analysis (MVA) of data applied
on the whole data (Figure1) showed
interesting differences in the mineral content
for the considered sites and harvesting
seasons. Lower content of Mn and Cd and
higher content of Zn, K and Cu were found
in sites A and B, respectively.
site A
site B
Figure1. OPLS-DA scores plot visualizing the
separation of the samples.
References
[1] Re Gomez-Becerra HF, Yazici A, Ozturk L, Budak H, Peleg Z, Morgounov A, Fahima T, Saranga Y,
Cakmak I. Genetic variation and environmental stability of grain mineral nutrient concentrations in
Triticum dicoccoides under five environments. Euphytica 2010, 171:39-52.
[2] Srinivasa J, Arun B, Mishra VK, Singh GP, Velu G, Babu R, Vasistha NK, Joshi AK. Zinc and iron
concentration QTL mapped in a Triticum spelta × T. aestivum cross. Theor Appl Genet 2014,
127:1643–1651
[3] Pasqualone A, Piarulli L, Mangini G, Gadaleta A, Blanco A, Simeone R. Quality characteristics of
parental lines of wheat mapping populations. Agric Food Sci 2015, 24: 118-127.