Download Lentiviral-Pooled-shRNA-library-Viral-Particles

Product Specifications
Human LVX-ZsGreen lentiviral pooled shRNA-mir screening library
Viral particles
The pooled shRNA screening library includes high titer viral particles and primers for the primary
PCR. The viral particles are ready to screen. The primers can be used to amplify the shRNA-mir
sequence from genomic DNA extracted from cells transduced with the constructs from the
screening library.
Note: The Pooled Screening NGS Illumina Platform Primer kit (TRP0001) may be purchased to adapt the
primary PCR product for analysis using the Illumina NGS platform.
Kit components:
The following viral particles are included:
Plasmid
Pooled shRNA screening library
Non-targeting control #1
The following oligonucleotides are included:
Primer
Primary PCR Forward primer
Primary PCR Reverse primer
Primer sequence
Primary PCR Forward
Primary PCR Reverse
Amount (TU/ml)
50 µl at > 108
25 µl at > 108
Concentration (µM)
Volume (µl)
10
2 x 400
10
2 x 400
5’ CAGAATCGTTGCCTGCACATCTTGGAAAC 3’
5’ CTGCTAAAGCGCATGCTCCAGACTGC 3’
Shipping and Storage
Primers are shipped dry ice. Viral particles should be stored at -80°C. Primers may be stored at
-20°C.
Contact Information
Technical support: 866.833.0712 or contact us at [email protected]
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For Research Use Only.