Download INVESTIGATING THE MICROBIAL COMMUNITY OF

REMEDIATE
INVESTIGATING THE MICROBIAL COMMUNITY OF PAHs AND
BTEX CONTAMINATED GROUNDWATER UNDERGOING NATURAL
ATTENUATION
IBRAHIM ISIAKA HUSSEIN
SUPERVISORS: PROF. MIKE LARKIN, DR CHRIS ALLEN AND
DR LEONID KULAKOV
MY PROFILE
NATIONALITY
 Nigerian - From Nasarawa State (North Central)
EDUCATIONAL BACKGROUND
 PND Food Science and Technology – Federal Polytechnic Bauchi, Nigeria – 2000
 Vocational Courses
 B.Tech (Hons) Applied Microbiology – Federal University of Technology Bauchi (FUTB/ATBU), Nigeria – 2008
 Isolation and Identification of Bacteria Associated with ‘Balangu’ (Roasted Meat Product) Sold within Bauchi Metropolis
 M.Sc Environmental Microbiology – University of Aberdeen (UoA), Scotland, UK – 2011
 Application of Bioluminescence-Based Microbial Biosensor for Diagnosis of Hydrocarbon in Groundwater Samples
 Bacterial Biosensor for Diagnostic Determination of Hydrocarbon in Refined Oil Product’s Contaminated Water Samples
 PhD Environmental Microbiology – Queen’s University Belfast (QUB), Northern Ireland, UK – 2015 – Till date
 Investigating the Microbial Community of PAHs and BTEX Contaminated Groundwater Undergoing Natural Attenuation
 PhD FOCUS
 Investigating the Microbial Community of PAH and BTEX Contaminated Groundwater Undergoing Natural
Attenuation - Supervision: Prof. Mike Larkin, Dr Chris Allen and Dr Leonid Kulakov
RESEARCH AIM AND OBJECTIVES
 Microbial community structure of PAH and BTEX contaminated groundwater
 Potential PAHs and BTEX degraders in the natural attenuation complex
 Microbial functional genes
 Scope of microbial biodiversity
 Microcosm approach for determining gene abundance and gene expression
 Microbial ecology of PAHs and BTEX contaminated environmental samples
RESEARCH APPROACH
 Sample collection
 Bacterial and Archaeal 16S rRNA Sequencing
o Characterization of microbial diversity
o In sight on microbial ecology
 Sequenced-Based Metagenomics - Captures massive information on the microbial community
o Genomics analysis of microbial population
o Isolated genomic library is sequenced with high-throughput sequence
 Functional Metagenomics - Detected specific functions of genes isolated
o By expressing the isolated genomic library
 Stable Isotope Probing – labelling with 13C
o Which organism is actively metabolizing carbon from the 13C
 Microcosm Experiment – Controlled Lab Experiment
o Abundant or dominant genes
o High or low Gene expression
RESEARCH APPROACH
Others include:
Phylogenic Analysis
o Also to infer the functionality of microbial community
DNA/RNA Extractions
Agarose gel electrophoresis
DNA/Gene Quantification DNA Amplification reaction – Especially for target DNA/RNA using specific Primers
o Quantitative Polymerase Chain Reaction (qPCR) – Quantitatively measure the amplification of DNA using Fluorescent probes
o Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) – Qualitatively detect gene expression via creation of cDNA from RNA
o PCR – DNA amplification
…… Scientific findings are expected based on these proposed approaches
REMEDIATE
Thanks For Listening
This project has received funding from the
European Union’s Horizon 2020 research and
innovation programme under the Marie
Skłodowska-Curie grant agreement No. 643087
Project coordinated by the QUESTOR Centre at
Queen’s University Belfast
www.qub.ac.uk/questor