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High frequency of Ikaros isoform 6 expression in acute myelomonocytic and monocytic leukemias: implications for up-regulation of the antiapoptotic protein Bcl-XL in leukemogenesis by Tomohito Yagi, Shigeyoshi Hibi, Mami Takanashi, Gen Kano, Yasuhiro Tabata, Toshihiko Imamura, Tohru Inaba, Akira Morimoto, Shinjiro Todo, and Shinsaku Imashuku Blood Volume 99(4):1350-1355 February 15, 2002 ©2002 by American Society of Hematology Pattern of Ikaros proteins expression in AML cases.(A) In the nested RT-PCR analysis, M indicates the 100-base pair (bp) ladder marker; lanes 1 to 7 represent FAB M4, M5a, and M5b cases; lanes 8 to 10; M2 cases; lanes 11 to 13; M7 cases. Tomohito Yagi et al. Blood 2002;99:1350-1355 ©2002 by American Society of Hematology Cell growth following induction of Ik6.Growth of cells from 32D/TREx/Ik6 clones (clone 7, 11, and 14) and 32D/TREx/empty. Tomohito Yagi et al. Blood 2002;99:1350-1355 ©2002 by American Society of Hematology Western blot analysis of Bcl-2, Bcl-XL, and STAT5 expression in 32D/TREx/Ik6 clone 11 growing in IL-3–deprived medium with or without tetracycline.Only Bcl-XL was up-regulated. Tomohito Yagi et al. Blood 2002;99:1350-1355 ©2002 by American Society of Hematology Western blot analysis of Ik2, Ik6, Bcl-2, Bcl-XL, and STAT5 expression in a Cos7/pRC/CMV/Ik2/TREx/Ik6 clone.Expression was tested before (lane 2) and at 12 hours (lane 3), 16 hours (lane 4), and 24 hours (lane 5) after Ik6 induction. Tomohito Yagi et al. Blood 2002;99:1350-1355 ©2002 by American Society of Hematology Semiquantification of Bcl-2, Bcl-XL, and STAT5 expression with a densitometric assay based on levels of β-actin expression.(A) Summary of data for three 32D/TREx/Ik6 clones. Tomohito Yagi et al. Blood 2002;99:1350-1355 ©2002 by American Society of Hematology Annexin-V/fluorescein isothiocyanate FACS analysis of apoptotic cell death.Representative data for 32D/TREx/Ik6 clone 11 are shown. Tomohito Yagi et al. Blood 2002;99:1350-1355 ©2002 by American Society of Hematology Subcellular localization of Ik2 and Ik6 proteins.Ik2 was localized in the chromatin structure of 32D cells expressing wild-type Ikaros proteins (A) and overexpressed IK2 (B), whereas both Ik2 and Ik6 were localized in the cytoplasm of 32D cells expressing I... Tomohito Yagi et al. Blood 2002;99:1350-1355 ©2002 by American Society of Hematology Growth curve of 32D cells with and without Bcl-XL overexpression under the IL-3–deprived medium condition.Growth of 32D cells with the overexpression of Bcl-XL was less inhibited, compared with that without the overexpression of Bcl-XL. Tomohito Yagi et al. Blood 2002;99:1350-1355 ©2002 by American Society of Hematology
