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RaMP 2016
Melissa MacDonald
Mentor: Mariana Stevens
PI: Aaron Zorn
Developmental Biology
What?
•
•
•
•
Xenopus laevis
In situ = “in place”
Expression → Regulation
Pathways
– Injection
– Transgenic animals (heat shock)
– Small molecules
In situ protocol
mRNA
A
A
C
G
C
Probe
Anti-DIG
Staining
C
U
G
A
A
G
U
A
C
U
G
DIG
In situ staining shows gut organs
Liver
Intestine
Stomach
for1
darmin
sox2
Lungs
Pancreas
ptf1a
nkx2
So What?
• Frog- Conservation of genes across species
(BMP, Wnt)
• Understand how the gut tube is patterned
Now What?
• Women in Science and Engineering Research
Experience for Women Undergraduates
• Summer project: in situ and PCR for novel
genes in the Xenopus genomes
References
• www.Xenbase.org
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