Download THE COMPLETE GENOME SEQUENCE OF COLUMBID

THE COMPLETE GENOME SEQUENCE OF COLUMBID CIRCOVIRUS, A VIRAL
GENOME RECOVERED FROM SERA OF BRAZILIAN PIGEONS
Tochetto, C.1,2; Loiko, R. M.1, 2; Varela, A. P. M. 1; Scheffer, C.1, 2; Paim, W. P.1, 2; Cerva, C. 1,2;
Lima, D. A.1, 2; Herpich, J. I.1; Bueno, T. S.2; Morel, A. P.3; Trainini, L. G.3; Mayer, F. Q2;
Roehe, P. M1*.
1
Laboratório de Virologia, Departamento de Microbiologia Imunologia e Parasitologia,
Instituto de Ciências Básicas da Saúde (ICBS), Universidade Federal do Rio Grande do Sul
(UFRGS), Porto Alegre, Rio Grande do Sul, Brazil. 2FEPAGRO Saúde Animal – Instituto de
Pesquisas Veterinárias Desidério Finamor (IPVDF) Eldorado do Sul, Rio Grande do Sul,
Brazil; 3HAYABUSA Falcoaria e Consultoria Ambiental, Brazil.
*e-mail: [email protected]
Columbid circovirus (CoCV), also known as pigeon circovirus (PiCV), is an
immunosuppressive agent that causes Young Pigeon Disease Syndrome (YPDS) in pigeons.
The CoCV has been reported in domesticated and urban pigeon populations worldwide. It is
classified within the family Circoviridae, genus Circovirus and has a small circular single
stranded DNA genome. In this study, we describe the complete genome sequence of columbid
circovirus (CoCV) from pigeon (Columba livia) samples in Brazil. Ten serum samples were
collected from apparently health urban pigeons in the municipality of Cambará, Paraná,
Brazil. Sera were pooled, filtered (0.45 um) and ultracentrifuged 44,000 rpm for four hours to
concentrate the viral population. Viral DNA was extracted using phenol-chloroform reagent
and enriched with phi29 DNA polymerase. The amplicons were purified with Agencourt
AMPure XP – PCR Purification and submitted to next generation sequencing in MiSeq
Illumina platform. A total of 325,182 reads were obtained. The reads were assembled on
BaseSpace Cloud by SPAdes Genome Assembler (version 3.5) and analyzed on Geneious
software (version 8.1.7). One circular contig containing 1,719 reads assembled displayed a
high genomic similarity (89.7%) with CoCV. The genome comprises 2,041 nucleotides
arranged in two main genes that encode a replication-associated protein ( rep, ORF V1) and
capsid protein (cp, ORF C1). An intergenic region of 97 nucleotides that separated these
genes comprises the putative origin of replication. The stem-loop structure and nanomer
sequence conserved in circovirus was deduced. This is the first report on CoCV complete
genome detected in Brazilian pigeons. Further studies will be performed in order to better
understand the virus’ biology and its epidemiology in other regions of Brazil.
Palavras-chaves: Pigeon circovirus, Columba livia.
Suporte financeiro: CAPES, Finep
Área de conhecimento: Microbiologia animal.