Download DNA Damage ELISA Kit

DNA Damage ELISA Kit
Quantifies oxidative damage on DNA by measuring 8-OHdG levels
Catalog No.
AS15 2887
AS15 2887X5
DNA Damage ELISA Kit Specifications
Purpose: Quantifies oxidative damage
on DNA by measuring 8-OHdG
levels
Species Reactivity: Species Independent - Detects
8-OHdG
Size
96-well
5 x 96-well
Advantages of competitive assay
for the quantification of 8-OH-dG in
urine, cell culture and plasma:
Sample Type: Urine
Cell Culture
Plasma
Other Sample Matrices
Sensitivity: 0.59 ng/mL
· quick incubation time of 60 minutes
· simple to use protocol
· stable components
· wider assay range
Assay Range: 0.94—60 ng/mL
Incubation Time: 60 minutes
Item
Product Citations: 30
Storage Temp: All reagents are stable as supplied
at 4°C, except the standard which
should be stored at -20°C
Typical standard curve
3
Average OD (450nm)
2,5
8-Hydroxy-2-Deoxy Guanosine: BSA
Coated Plate
96 wells
Quantity/
Size
1 Plate
8-Hydroxy-2-Deoxy Guanosine
standard
1 vial/100µl
8-Hydroxy-2-Deoxy Guanosine HRP
Conjugated Monoclonal Antibody
1 vial/75µl
Sample and Standard Diluent (Red)
1 vial/50ml
8-Hydroxy-2-Deoxy Guanosine
Antibody Diluent (Blue)
1 vial/13ml
Wash Buffer Concentrate (10X)
1 vial/50ml
TMB Substrate
1 vial 13ml
Stop Solution
1 vial/13ml
2
1,5
1
Plate Cover
0,5
0
0,1
1
10
2
100
8-OHdG (ng/ml)
For more products and information, please visit www.agrisera.com
www.agrisera.com | [email protected] | Ph: +46 935 33000, Fax: +46 935 33044
DNA Damage ELISA Kit
Quantifies oxidative damage on DNA by measuring 8-OHdG levels
Easy to use!
Empty Well
Catalog No.
AS15 2887
AS15 2887X5
Add 50µl
Diluted Sample
Size
96-well
5 x 96-well
Add 50µl
Diluted Antibody
High Sample [8-OHdG]
Incubate
60 min
4X Wash
Incubate
30 min
Add 100µl
Stop Solution
Plate bound 8-OHdG
Low Sample [8OHdG]
!
Add 100µl TMB
Sample 8-OHdG
TMB
8-OHdG Monoclonal Antibody - HRP Conjugated
Not for human or animal disease diagnosis or
WARNING: therapeutic drug use.
www.agrisera.com | [email protected] | Ph: +46 935 33000, Fax: +46 935 33044