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From: Retinoblastoma Protein Purification and Transduction of Retina and Retinoblastoma Cells Using Improved
Alphavirus Vectors
Invest. Ophthalmol. Vis. Sci.. 2004;45(9):3320-3329. doi:10.1167/iovs.04-0140
Figure Legend:
pSMART is an efficient replicon. (A) Assessment of different producer–target cell lines. Virus was collected 24 hours
after cotransfection of HEK-293 derivative (Phoenix-Eco), BHK-21, HEK-293, and NG108 cells with pSCAβ and
pSCAHelper. The highest titer was obtained on BHK-21 cells except for virus generated in NG108 cells, which had the
highest titer in HEK-293 cells. (B) The SFV capsid protein in pSMART functions as a translation enhancer. Twenty-four
hours after transfection into HEK-293 cells pSMART-LacZ, containing the capsid, produced approximately eight times
more β-galactosidase per cell than pSCAβ, which lacks the capsid. In replicon-transfected cells, the presence of
pSCAHelper
did8/10/2017
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produced approximately three times more protein than SCAβ virus per BHK-21 cell 18 hours after transduction. (C) The