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From: Two Bioactive Molecular Weight Fractions of a Conditioned Medium Enhance RPE Cell Survival on Age-Related
Macular Degeneration and Aged Bruch's Membrane
Trans. Vis. Sci. Tech.. 2016;5(1):8. doi:10.1167/tvst.5.1.8
Figure Legend:
Comparison of RPE cell morphology after 21-day culture in 30- (A, B) and 50-kDa filtrates (C, D). (A) Predominantly
large and flat RPE cells resurfaced half of the explant only. Cell surfaces were smooth with no or few short apical
processes (inset). (B) RPE cells resurfacing the explant were mostly monolayered, large, and flat. The nuclear density
was 17.65 ± 0.68 nuclei/mm Bruch's membrane. (C) RPE cells resurfacing the explant were largely multilayered with
small defects in the RPE cell layer. Surface cells of the multilayer were large and flat. Small cells could be seen between
areas of multilayering. Arrowheads point to the edge of a multilayer surrounding small cells. High magnification inset
illustrates
the variability
in RPE cell size
the expression
of apical
processes
on cell surfaces.
(D) ©
The
explant
wasreserved.
Date
of download:
8/10/2017
The and
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2017.
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resurfaced by RPE cells that were highly variable in morphology. Although many cells were small, they were mainly flat