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Supplementary Material
Supplementary Table 1. Antibodies used to investigate the co-localization of
TFFs and mucins. All controls consisted of human FFPE material. (MM = mouse
monoclonal; RP = rabbit polyclonal; C = HIER with 10mM sodium citrate pH 6.0;
E = HIER with 1mM EDTA pH 8.0).
Antibody
Details
Source
Dilution
TFF1
pS2 Clone GE2
MM IgG11
hSP, clone GE16C
MM, IgM purified or
culture supernatant2
H00007033-MO1
Clone 3D9 MM IgG1
Rb anti-human GKN23
RP
NCL-MUC-1 Clone
Ma695 MM IgG1
NCL-MUC-2 Clone
Ccp58 MM IgG1
NCL-MUC-5AC Clone
CLH2 MM IgG1
NCL-MUC-6
Clone CLH5 MM IgG1
Ab65454 RP
G Elia,
CRUK
G Elia,
CRUK/
Abcam
AbNova
1:400 C
TFF2
TFF3
GKN2
MUC1
MUC2
MUC5AC
MUC6
Insulin (IF study)
Glucagon (IF
study)
Insulin (Ki67
study)
Glucagon (Ki67
study)
Ki67
Insulin/glucagon
secondary (IF)
TFF3 secondary
(IF)
Rabbit antimouse IgG biotin
Swine anti-rabbit
IgG biotin
Streptavidin-HRP
3, 3’ Di-amino
benzidine
Positive
control
stomach
1:10 E/
1:50 C
stomach
1:300 C
colon
WR Otto,
CRUK
Novocastra
1:200 C
stomach
1:100 C
Novocastra
1:100 C
Novocastra
1:50
normal
pancreas
small intestine
colon
stomach
Novocastra
1:50
stomach
Abcam
1:10 C
Ab9379 RP
Abcam
1:50 C
Clone 2D11-H5
MM IgG1
Clone IMD7
MM IgG1
Clone Mib1,
MM IgG1
Goat anti-rabbit
AlexaFluor 488 or Cy3
Goat anti-mouse
AlexaFluor 488 or Cy3
E0354 RP
Abcam
1:80
Abcam
1:1300
Dako
1:100
normal
pancreas
normal
pancreas
normal
pancreas
normal
pancreas
normal gut
Molecular
Probes
Molecular
Probes
DAKO
1:200
n/a
1:100
n/a
E0353
DAKO
1:300
P0397
IMMPACT DAB
As manufacturer’s
instructions
DAKO
Vector
1:500
1:300
1
Supplementary References
1. Williams R, Elia G, Stamp GW, et al. Characterization of monoclonal
antibodies raised to C-terminal peptides of pS2: a major trefoil peptide and
motility factor expressed in adenocarcinomas and regions of mucosal injury. Hum
Pathol. 1996;27:1259-1266.
2. Elia G, Chinery R, Hanby AM, et al. The production and characterization of a
new monoclonal antibody to the trefoil peptide human spasmolytic polypeptide.
Histochem J. 1994;26:644-647.
3. Otto WR, Patel K, McKinnell I, et al. Identification of blottin: a novel gastric
trefoil factor family-2 binding protein. Proteomics. 2006;6:4235-4245.
2