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In vitro differentiation of endothelial cells from AC133positive progenitor cells by Ursula M. Gehling, Süleyman Ergün, Udo Schumacher, Christoph Wagener, Klaus Pantel, Marcus Otte, Gunter Schuch, Philippe Schafhausen, Thorsten Mende, Nerbil Kilic, Katrin Kluge, Birgit Schäfer, Dieter K. Hossfeld, and Walter Fiedler Blood Volume 95(10):3106-3112 May 15, 2000 ©2000 by American Society of Hematology Growth curves of nonadherent cells generated from AC133+ progenitor cells in liquid cultures with stem cell growth factor and vascular endothelial growth factor.Proliferating nonadherent cells were obtained with the supernatant, and the number of cells per ... Ursula M. Gehling et al. Blood 2000;95:3106-3112 ©2000 by American Society of Hematology Morphology of adherent cells cultured for 14 days in liquid cultures in the presence of stem cell growth factor and vascular endothelial growth factor.All photographs are taken from the same experiment. Ursula M. Gehling et al. Blood 2000;95:3106-3112 ©2000 by American Society of Hematology Morphology of colonies generated from AC133-derived cells.In parallel experiments, cells cultured for 8 days (day 8 cells, A and B) and 14 days (day 14 cells, C and D) in liquid cultures with stem cell growth factor (SCGF) and vascular endothelial growth fa... Ursula M. Gehling et al. Blood 2000;95:3106-3112 ©2000 by American Society of Hematology Comparison of the clonogenic potential of freshly isolated AC133+ cells (day 0 cells) and AC133derived proliferating cells cultured for 8 days (day 8 cells) and 14 days (day 14 cells), respectively, in liquid culture with stem cell growth factor (SCGF) and... Ursula M. Gehling et al. Blood 2000;95:3106-3112 ©2000 by American Society of Hematology Immunocytochemical analysis of AC133-derived adherent cells using the nickel-glucose peroxidase anti-peroxidase technique.(A) Negative control (AC133-derived adherent cells cultured for 14 days in the presence of stem cell growth factor [SCGF] and vascular ... Ursula M. Gehling et al. Blood 2000;95:3106-3112 ©2000 by American Society of Hematology Immunocytochemical analysis of colonies grown from day 8 cells in semisolid media.Day 8 cells were cultured for 14 days in methycellulose supplemented with stem cell growth factor and vascular endothelial growth factor. Ursula M. Gehling et al. Blood 2000;95:3106-3112 ©2000 by American Society of Hematology Transmission electron microscopy photomicrographs of adherent cells cultured in liquid cultures with stem cell growth factor and vascular endothelial growth factor for 14 days.Singlemembrane bound structures, identified as Weibel-Palade bodies, are indicat... Ursula M. Gehling et al. Blood 2000;95:3106-3112 ©2000 by American Society of Hematology Histochemical staining of tumor grown in mice with severe combined immunodeficiency with the human-specific anti-CEACAM1 monoclonal antibody 4D1/C2.Panel (A) shows negative staining with 4D1/C2 in tumors grown after subcutaneous injection of A549 lung cance... Ursula M. Gehling et al. Blood 2000;95:3106-3112 ©2000 by American Society of Hematology
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