Download Supplementary Table 1 (doc 61K)

Supplementary Table 1. Nucleotide sequences of primers and amplicon size used for PCR amplification.
Gene
Forward primers
(5´to 3´)
Reverse primers
(5´to 3´)
Amplicon
size (pb)
Brown markers
Cidea
TCAGACCCTAAGAGACAACACA
CATTGAGACAGCCGAGGA
164
Ebf3
ATGACCAAACAGGCCATCGT
GGGTCTGAGGGTGTTTCGTT
140
Eva1
GGTCGTGAGCAGTTTGTATTC
CAGGTGTATGTCCCGTTGTC
158
Fbxo31
AGATCACCACAGGATGTCCG
GTAGGTCAGGCAGTTGTCGT
160
Fgf21
ACAGATGACGACCAGGACAC
AGGCTTTGACACCCAGGATT
145
Fndc5
ATGAAGGAGATGGGGAGGAA
GCGGCAGAAGAGAGCTATAACA
102
Pgc1a
CATTTGATGCACTGACAGATGGA
CCGTCAGGCATGGAGGAA
70
Prdm16
ACGACCACCTCTGCTACCTC
GGGCTCCTATTTTTGACCTTCT
216
Tbx15
GGATGAGACAGGTGGTCAGTT
CACAGGCACAGGTTTGGT
164
Ucp1
GGGCTGATTCCTTTTGGTCT
GGTGGTGATGGTCCCTAAGA
229
Cd137
AAGCAACCATTTAAGAAGGCGG
GGATGGCACATTACAGCTCG
115
Hoxc9
CGGCAGCAAGCACAAAGA
AGAAACTCCTTCTCCAGTTCCA
138
Slc27a1
CAGGAGTGGAGGGGAAAG
CAGAAGACGCAGGAAGATGG
131
Tbx1
ACCCTCGAAAAGACAGCGAG
TGCGTGATCCGGTGATTCTG
109
Tmem26
GCTGTTCCTGTTGCATTCCC
GGAGAAAGCCATTTGTAGCCTC
159
Brite markers
Mitochondrial biogenesis
Nrf1
CGAAAGAGACAGCAGACACG
TTGAAGACAGGGTTGGGTTT
125
Nrf2
CAGAGCAAGTGACGAGATGG
CCGAAATGTTGAGTGTGGTG
177
Tfam
GCTTTCGTGGCTGTGTGTAG
CAGGCGAGGGTATGCGG
176
DNA determination
B2m
CGTCTCTCGGGAAAGGAGTG
GCATACTCGCAAACAGCCAC
90
Mt complete
CCTCACAACACACCCCCTAC
ATTGGAGCTTCTACGTGGGC
135
CCGCACAAGGCAAATACATC
GACTCTCTGAACCGTCATCAA
159
genome
Reference gene
Gdi1
For brown markers: Cidea: cell death-inducing DNA fragmentation factor-α-like effector A; Ebf3: early B cell
factor-3; Eva1: epithelial V-like antigen 1; Fbxo31: F-box protein 31; Fgf21: fibroblast growth factor 21;
Fndc5: fibronectin type III domain containing 5; Pgc1a: peroxisome proliferator-activated receptor gamma
coactivator 1-alpha; Prdm16: PR domain containing protein-16; Tbx15: transcription factor T-box 15 and
Ucp1: uncoupling protein 1. For brite markers: Cd137: also known as Tnfrsf9, tumor necrosis factor receptor
superfamily, member 9; Hoxc9: homeo box C9; Slc27a1: solute carrier family 27 (FATP1 fatty acid
transporter), member 1; Tbx1: transcription factor T-box 1 and Tmem26: transmembrane protein 26. For
mitochondrial biogenesis: Nrf1: nuclear respiratory factor 1; Nrf2: nuclear respiratory factor 2 and Tfam:
transcription factor A, mitochondrial. For DNA determination: B2m: beta-2 microglobulin, used as marker of
nuclear DNA; Mt (mitochondrion) complete genome (Rattus norvegicus), used as marker of mtDNA.
Reference gene: Gdi1: guanosine diphosphate dissociation inhibitor 1.