Download Supplementary Table S3. Phospho-protein array data Inhibition of

Inhibition of tumor growth and metastasis in non-small cell lung cancer by LY2801653, an inhibitor of several
oncokinases, including MET
Corresponding author: Wenjuan Wu
Supplementary Table S3. Phospho-protein array data
Inhibition of phospho-proteins in H441 lung orthotopic tumor tissues treated with LY2801653.
Tumor lysates from vehicle group (n = 5) or 12-mg/kg LY2801653-treated group (n=3) in H441
lung orthotopic tumors were assessed with reverse-phase protein microarray for phosphorylation
status of various signaling pathway proteins. 39 phosphoprotein analytes were evaluated. The
log2 of the geometric mean of the 4 measurements per tumor (duplicate lysate per tumor and
each lysate evaluated twice) was used for subsequent analysis. Treatment group was compared to
the vehicle control group as “Inhibition%”. The statistic analysis (FDR, false discovery rate) is
described in Materials and Methods. The 14 analytes with significant FDRq values are shown in
Table 1. The data for the other 25 analytes are shown here.
Target
Protein
β-Actin
Phosphorylation
site(s)
NA
Inhibition%
FDR q Value
0.91
0.88
AKT
T308
9.67
0.21
AKT
S473
-10.60
0.31
ABL1
T735
6.75
0.19
ABL1
Y412
11.52
0.30
EGFR
Y845
9.40
0.28
EGFR
Y1045
26.07
0.18
EGFR
Y1068
2.49
0.86
ERBB2
Y1248
1.23
0.90
ERBB3
Y1289
8.46
0.27
PTK2
Y397
-0.61
0.96
Inhibition of tumor growth and metastasis in non-small cell lung cancer by LY2801653, an inhibitor of several
oncokinases, including MET
Corresponding author: Wenjuan Wu
FLT3
Y842
17.35
0.13
GAB1
Y627
19.36
0.12
GSK3A/B
S21/9
16.48
0.21
Y1135/36,
12.18
0.21
Y1131/Y1146
-1.61
0.87
mTOR
S2448
-0.74
0.90
mTOR
S4281
12.61
0.19
PDGFRβ
Y751
9.91
0.55
PKCAB
T638/T641
-16.23
0.19
RAF1
S338
15.53
0.18
RasGRF1
S916
17.88
0.23
SRC family
Y416
16.38
0.49
KDR
Y951
5.07
0.66
KDR
Y996
-10.34
0.50
KDR
Y1175
1.91
0.87
IGF1R
Y1150/51
IGF1R β/INSR