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SUPPORTING INFORMATION LEGENDS Table S1. Degenerated primers used to amplify regions Y8, Y5, Zg, C3’, and CM from bisulfite treated DNA (Y = G or A; R = C or T; N = I = G, C, T or A). Figure S1. Methylation at individual cytosine resolution of region Y5 of locus Yccat500 for individual clones from X21X21/Yccat500Yccat500/ZCZC and --/Yccat500Yccat500/ZC* plants. Results from CyMATE; filled symbols, cytosine methylation; open symbols, lack of methylation; red circles, CG; blue squares, CHG; green triangles, CHH. Figure S2. Methylation at individual cytosine resolution of region Zg of locus ZC for individual clones from X21X21/Yccat500Yccat500/ZCZC, --/Yccat500Yccat500/ZC* and X21X21/--/ZCZC plants. Results from CyMATE; filled symbols, cytosine methylation; open symbols, lack of methylation; red circles, CG; blue squares, CHG; green triangles, CHH. Figure S3. Methylation at individual cytosine resolution of region C3’ of CAT2 for individual clones from X21X21/Yccat500Yccat500/ZCZC, --/Yccat500Yccat500/ZC*, and X21X21/--/ZCZC plants. Results from CyMATE; filled symbols, cytosine methylation; open symbols, lack of methylation; red circles, CG; blue squares, CHG; green triangles, CHH. Figure S4. Methylation at individual cytosine resolution of region Y8 of locus Y11 for individual clones from X21X21/Y11Y11 and --/Y11Y11 plants. Results from CyMATE; filled symbols, cytosine methylation; open symbols, lack of methylation; red circles, CG; blue squares, CHG; green triangles, CHH. Figure S5. Methylation at individual cytosine resolution of region C3’ of CAT2 for individual clones from X21X21/Y11Y11, --/Y11Y11 and X21X21/-- plants. Results from CyMATE; filled symbols, cytosine methylation; open symbols, lack of methylation; red circles, CG; blue squares, CHG; green triangles, CHH. Figure S6. Methylation at individual cytosine resolution of region C3’ of CAT2 for individual clones from Xccat800hpXccat800hp and wild-type Col-0 plants. Results from CyMATE; filled symbols, cytosine methylation; open symbols, lack of methylation; red circles, CG; blue squares, CHG; green triangles, CHH. Figure S7. Methylation at individual cytosine resolution of region CM of CAT2 for individual clones from Xccat800hpXccat800hp and wild-type Col-0 plants. Results from CyMATE; filled symbols, cytosine methylation; open symbols, lack of methylation; red circles, CG; blue squares, CHG; green triangles, CHH.