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Download 1. DNA SEQUENCER (Applied Biosystems, 3730xl DNA Analyzer)
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1. DNA SEQUENCER (Applied Biosystems, 3730xl DNA Analyzer) DNA Sequencer is a method of determining the order of nucleotide bases such as adenine, guanine, cytosine and thymine in a DNA oligonucleotide. It is useful in basic research studying fundamental biological processes in diagnostic or forensic research. We have the 96-capillary 3730xl DNA Analyzer as the Gold Standard for high throughput genetic analysis. Able to optimized polymers and increase your productivity without compromising your results and perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation. With the 3730xl DNA Analyzer, you get the highest-quality data from a wide array of applications. Perform on POP-7™ Polymer with Data Collection Software v3.0. REQUIREMENT Perform all the purified and cycle sequencing method or any method necessary. Adequate DNA quantity. Location : INFRA Advanced Laboratory at Level G, RMIC Building 2. RT-PCR (Applied Biosystems, QuantStudio™ 12K Flex) Real-time PCR is an advanced form of the Polymerase Chain Reaction that maximizes the potential of the technique. Combining flexible throughput capabilities with a streamlined workflow, the QuantStudio™ 12K Flex system takes you from targeted discovery through confirmation and screening, all on a single platform. Increase your throughput by as much as 32-fold by running 4 OpenArray® plates in a single run on the QuantStudio™ 12K Flex system. It's the equivalent of 32 traditional 384-well qPCR plates or 12,000 data points. REQUIREMENT Sample must be DNA or RNA. Specialized consumables to be used, appropriate for the sample blocks of the QuantStudio 12K Flex system: Micro Amp 8-Tube Strips (0.1 ml) Micro Amp Optical 96 – Well fast reaction plate with bar code Micro Amp Optical 384 – Well reaction plate with bar code Array card Location : INFRA Advanced Laboratory at Level G, RMIC Building 3. RHEOMETER (TA Instrument, DHR-2) Rheometer is an instrument that measures both viscosity and viscoelasticity of semi-solid sample. It is also provides Transient response (relaxation modulus, creep compliance, creep recovery) information of the material. TECHNICAL SPECIFICATION Geometry provided : 40mm flat, 40mm cone, 60mm cone Temperature range between 0-200 0C Peltier plate usage only REQUIREMENT No Acidic, strong alkaline and chlorinated sample. Semi-solid sample only. No cured samples. Stable state of samples. High amount of samples (depend on type of geometry). Location : INFRA Advanced Laboratory at Level G, RMIC Building 4. TENSIOMETER (Attension, Sigma 700) Tensiometer is a precise characterization of a number of material properties. Analysis of surface and interfacial tension, powder wettability, dynamic contact angles, critical micelle concentration, density and sedimentation. REQUIREMENT Parameter/characteristic must be provided. Liquid and soluble sample. Homogenous sample. Location : INFRA Advanced Laboratory at Level G, RMIC Building 5. ELLIPSOMETER (J.A. Woollam M-2000V) Ellipsometer is a non-destructive, light optical analysis technique. Ellipsometry measures the change in polarization state of light reflected from a surface of a material. Measuring tools for film thickness, optical constant & change in polarization. Ellipsometer is widely use in thin film identification. TECHNICAL SPECIFICATION White lamp source, Quartz Tungsten Halogen Lamp, 3000hrs Wave length 370nm-5000nm Complete EASE Software REQUIREMENT Sample must be in solid form and fit the sample holder. Sample must be homogenous and has a flat surface. Location : INFRA Advanced Laboratory at Level G, RMIC Building 6. PARTICLE IMAGE VELOCIMETRY (PIV) (Dantec Dynamics Nano L135-15PIV) Particle Image Velocimetry (PIV) is a whole-flow-field technique providing instantaneous velocity vector measurements in a cross-section of a flow. Two velocity components are measured, but use of a stereoscopic approach permits all three velocity components to be recorded, resulting in instantaneous 3D velocity vectors for the whole area. PIV is widely used in many application such as fluid & solid mechanics, Hydraulics & hydrodynamics, material research and etc. TECHNICAL SPECIFICATION Dynamic Studio v3.31 software 2 camera (2D Experiment & 3d Experiment) Laser system (Dual power 135-15) REQUIREMENT Experiment set-up must be appropriate with the room size and doesn’t affect the environment cleanliness. User should have knowledge on the operation & data analysis. Training by Application Engineer from Alam Meridian (M) Sdn. Bhd. available at customer own cost & arrangement. Location : INFRA Analysis Laboratory at Level 3, RMIC Building OFFICER IN-CHARGE 1. DNA SEQUENCER (Applied Biosystems, 3730xl DNA Analyzer) 2. RT-PCR (Applied Biosystems, QuantStudio™ 12K Flex) Jauhar Aisyah Junaidi Science Officer Contact: 03-7967 7022 (Ext: 2320/2327) Email: [email protected] 3. RHEOMETER (TA Instrument, DHR-2) 4. TENSIOMETER (Attension, Sigma 700) Hasbullah Ibrahim Science Officer Contact : 03-7967 7022 (Ext: 2327) Email : [email protected] 5. ELLIPSOMETER (J.A. Woollam M-2000V) 6. PARTICLE IMAGE VELOCIMETRY (PIV) (Dantec Dynamics Nano L135-15PIV) Mardiana Said Senior Science Officer Contact : 03-7967 4619 Email : [email protected] RENTAL CHARGES UM CUSTOMER EQUIPMENT DNA SEQUENCER RT-PCR RHEOMETER TENSIOMETER ELLIPSOMETER PARTICLE IMAGE VELOCIMETRY Per day (RM) 800.00 250.00 120.00 60.00 200.00 180.00 Per month (RM) 16,000.00 5000.00 2400.00 1200.00 4000.00 3600.00 Per year (RM) 192,000.00 60,000.00 28,800.00 14,400.00 48,000.00 43,200.00 NON-UM CUSTOMER EQUIPMENT DNA SEQUENCER RT-PCR RHEOMETER TENSIOMETER ELLIPSOMETER PARTICLE IMAGE VELOCIMETRY Per day (RM) 1040.00 325.00 156.00 78.00 260.00 234.00 Per month (RM) 20,800.00 6500.00 3120.00 1560.00 5200.00 4680.00 Per year (RM) 249,600.00 78,000.00 37,440.00 18,720.00 62,400.00 56,160.00 * Price for Non-UM Customer is subjected to 6% GST TERMS & CONDITIONS: I. II. III. IV. V. VI. VII. VIII. IX. X. XI. XII. Submission of request form and proof of payment/journal transfer must be made before any booking of slot (rental). Validity of rental : i) Per day rental is valid for 1 month only, effective from the date of submission of ‘invoice commitment’/ receipt/ proof of payment. Staff in-charge will record in log book as reference. ii) Per month & per year rental valid for 1 year only, effective from the date of submission of ‘invoice commitment’/ receipt/ proof of payment. Staff in-charge will record in log book as reference. The rental of equipment is only valid for specified analysis, slot, location & purposes only. No exchange on the analysis or ownership allowed. Analysis is only allowed within working hours (8.30 am to 5.00 pm, Monday-Friday). Reservation/ booking for rental should be made on a weekly basis, subject to availability of slots and equipment. List of user & letter of liability (with signature of each user) must be submitted along with the request form and proof of payment/journal transfer. User should have knowledge on the operation & data analysis of the equipment before conducting any experiment. Brief training session (1 time-max: 1 hour) is available for new customer only by officer/staff in-charge before conducting the experiment. Additional training from third party is at customer’s own cost and arrangement upon approval from officer in-charge. Sample preparation & consumables are not included. Rental charges are not applicable with other promotion or discount. Slot will be based on availability. Staff in-charge should be informed at least 48 hours on any cancellation or change of slot before the booked date. Failure to do so, payment made will be forfeited. Experiment set-up must be reset to default after every use and user must ensure the cleanliness of working area in compliance with laboratory safety procedures & guidelines. The rental rate is effective from 17th April 2017. All terms and conditions are subject to change without prior notice.