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Human ESC-derived hemogenic endothelial cells undergo distinct waves of endothelial to hematopoietic transition by Shahin Rafii, Christopher C. Kloss, Jason M. Butler, Michael Ginsberg, Eric Gars, Raphael Lis, Qiansheng Zhan, Pavle Josipovic, Bi-Sen Ding, Jenny Xiang, Olivier Elemento, Nikica Zaninovic, Zev Rosenwaks, Michel Sadelain, Jeremie A. Rafii, and Daylon James Blood Volume 121(5):770-780 January 31, 2013 ©2013 by American Society of Hematology Hemato-endothelial differentiation of hESCs in serum-free conditions is augmented by coculture with vascular feeder cells. Shahin Rafii et al. Blood 2013;121:770-780 ©2013 by American Society of Hematology Hematopoietic derivatives of transgenic hESCs express the CD41a-GFP and originate from VPrmOrange+ hemogenic ECs. (A) hESCs that were transduced with the vascular specific reporter transgene (VPr-mOrange) as well as a hematopoietic specific transgene (CD41... Shahin Rafii et al. Blood 2013;121:770-780 ©2013 by American Society of Hematology The CD41a-GFP dimLineage− population is composed of multipotent hematopoietic progenitors. Shahin Rafii et al. Blood 2013;121:770-780 ©2013 by American Society of Hematology Expression of Dll4 ligand on vascular feeder cells specifically promotes myeloid differentiation of HPCs. (A-B) The CD43+ population was isolated from hESC differentiation cultures at day 12 and cultured for an additional 7 days in either the absence (plast... Shahin Rafii et al. Blood 2013;121:770-780 ©2013 by American Society of Hematology Lineage potential of hemogenic ECs varies with the stage of hESC differentiation. Shahin Rafii et al. Blood 2013;121:770-780 ©2013 by American Society of Hematology
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