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μg aSyn expressed per 10 cm dish A C absorbance signal @ 405 nm 2.5 r2=0.99 2.0 1.5 1.0 0.5 75 r2=0.99 60 45 30 15 0.0 500 1000 1500 0.25 2000 B aSyn concentration (pg/μl) aSyn pg / 50 μl (well) 100 # 0.50 # # 80 60 40 20 0 1 in 2 k 0.25 1 in 4 k 1 in 200 1 in 800 1 in 2 k 0.5 DNA μg/dish Supplementary Figure 1 1.00 μg SCNA DNA transfected / 10 cm dish # 1 in 200 1 in 800 0.75 1 in 4 k 1 in 200 1 in 800 1 in 2 k 1 1 in 4 k