Download Table S1: Correlation of Gene Expression Using Suberin Associated

Table S1: Correlation of Gene Expression Using Suberin Associated Genes as Bait: Some
Representative Results
Atg
Gene Name
Locus
or Function
(r2/n)b
Pearson Coefficient (r2 value)
GPAT5
CYP86A1
Bait gene
Bait gene
ASFT
CYP86B1
Bait set and selected BAHD genes
At3g11430a
GPAT5
1.000
0.631
0.583
0.679
0.631
At5g58860a
CYP86A1
0.679
1.000
0.766
0.714
0.720
At5g41040a
BAHD (ASFT)
0.583
0.766
1.000
0.664
0.671
At5g63560
BAHD
0.632
0.885
0.676
0.754
0.737
At3g48720
BAHD
0.115
0.057
0.059
0.081
At4g24510
BAHD (CER2)
0.186
0.215
0.111
0.244
At5g48930
BAHD (HCT)
0.062
0.082
0.094
0.083
0.080
Genes with strong correlation to suberin bait set
At5g09520
Hydroxyproline-
0.693
0.831
0.815
0.756
0.774
0.620
0.873
0.886
0.722
0.775
rich protein
At2g48140
Lipid transfer
protein
At1g68850
Peroxidase
0.715
0.867
0.783
0.880
0.811
At2g21100
Dirigent protein
0.609
0.782
0.801
0.740
0.733
At1g74460a
GDSL lipase
0.603
0.921
0.828
0.707
0.765
At3g44540
Acyl reductase
0.693
0.879
0.728
0.765
0.766
At5g23190
CYP86B1
0.679
0.714
0.664
1.000
0.686
At5g13580
ABC
0.780
0.759
0.750
0.657
0.737
Controls – cutin related genes
At4g00360
ATT1 CYP86A2
0.008
0.000
0.002
0.004
At1g17840
ABC WBC11
0.165
0.234
0.138
0.181
At1g01610
GPAT4
0.022
0.039
0.039
0.028
At4g00400
GPAT8
0.045
0.024
0.018
0.058
At1g49430*
LACS2
0.007
0.000
0.002
0.009
0.005
0.032
Other genes of interest
At5g08250
CYP86B2
0.412
0.206
0.236
0.308
0.291
At3g14680
CYP72A14
0.470
0.679
0.638
0.555
0.586
At5g05390
Laccase
0.123
0.066
0.091
0.117
0.099
At3g22400
Lipoxygenase
0.295
0.239
0.271
0.433
0.310
At2g40890
3’-Hydroxylase
0.173
0.301
0.331
0.269
0.269
At4g26220
3’-OH Methylation 0.357
0.523
0.667
0.395
0.486
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a
Annotated as “relevant candidate genes in suberin synthesis and cork regulation” by Soler et al. (2007).
b
Self-correlation not included
Co-response Analysis: The Cress-express tool (http://www.cressexpress.org/) was used to evaluate gene
coexpression between genes of the bait set or studied in this work and all genes from about 1800
Affymetrix Ath1 arrays in the database. All tissue types were included, but arrays for specific experiments
were not chosen. The square of Pearson's correlation is given as a score for the measure of
coexpression relationship between two genes. Negative transcriptional correlations are highlighted in
yellow. Where correlations are positive, the average value of r2 across the four genes with demonstrated
roles in suberin biosynthesis, namely GPAT5, CYP86A1, CYP86B1 and ASFT, are shown in the right
hand column. The list of candidate genes on the left hand column is not comprehensive, but genes with
average (r2/n) values above about 0.6 may have specific roles in suberization.